Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"immunogold"”.
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A Morphological Study of Paracrine Interactions in the Anterior Pituitary of Lactating Rats Using Immunogold Techniques
… levels of lactational stimuli. Using specific immunogold staining and TEM, cellular associations between luteotropes and lactotropes were quantitated. The average number of cell associations for five study groups was determined to be 2.43 $\pm$ 0.03 for the control groups, 2.55 $\pm$ 0.11 for …
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Analyzing large-scale chromatin fiber via studying cytology of DNA replication and in vivo immunogold labeling
… predecondensation. The potentials of new in vivo immunogold labeling techniques suggested the possibility of studying chromosome structure with specificity by electron microscopy.
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Structure and Composition of Postsynaptic Densities
… from these regions was then compared through immunogold analysis and electron tomography. Tomographic reconstructions revealed that while the majority of PSDs shared a similar dense protein organization, there were cerebellar PSDs which displayed a latticelike protein organization. PSDs from …
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Subcellular labelling of myelin protein (PLP and DM20) in the central nervous system
… in sections that were not contrast stained. The immunogold technique was applied to sections from ventral columns in cervical spinal cord which has a dense population of medium to large myelinated axons. Assessments were made using anti-PLP antibodies in mature animals (with PLP at its peak of …
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The study of cell surface components on Porphyromonas gingivalis
… of the native PgAg1 molecule was demonstrated by immunogold labelling of intact P. gingivalis cells using antibodies raised to the recombinant protein (arPgAg1). The recombinant protein was purified to homogeneity, and the native PgAg1 protein partially purified from P. gingivalis. The …
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A light and electron microscope investigation of the host-parasite relationship in the brains of mice with congenital toxoplasmosis.
… antiToxoplasma IgG as the primary layer in immunogold staining, revealed that Toxoplasma antigenwas widely distributed within the matrix of the cyst, being most concentrated in the proximity of the inner surface of the parasitophorous vacuole membrane. Relatively little Toxoplasma antigen …
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Investigation and development of techniques for the characterisation of the synthetic/biological interface
… in promoting cell spreading on surfaces. The immunogold labelling technique indicated that there were small, but noticeable differences, between the distribution of proteins on a range of surfaces. This study has shown that surface analysis techniques have different sensitivities in terms of …
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The cross-linking mechanism of filamin A in the actin cytoskeleton
… to actin was compared to filamin in solution and immunogold molecules bound along the filamin rod were used to map the 3-D organization of filamin-actin junctions. There is evidence that filamin binds to actin at more sites than filamin's two established N-terminal actin-binding sites. These …
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Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana
… of analysis: promoter reporter experiments and immunogold localization by electron microscopy. XCP2 may be involved in catalyzing the degeneration of the protoplast during the final autolytic stages of tracheary element differentiation. To this date XCP2 function has not been directly …
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Korrelative licht- und elektronenmikroskopische Untersuchungen zu Tonoplast intrinsischen Proteinen als Merkmale verschiedener Vakuolentypen
… innerhalb der Wurzeln ermittelt, während die Immunogold-Markierung bei wesentlich höherer Auflösung der genauen Lokalisation innerhalb der Zellen diente. Diese korrelative Studie ermöglichte somit eine sehr differenzierte Auswertung bei optimaler Auflösung. Dadurch konnte gezeigt werden, dass …
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Influence of ApoE polymorphism on synaptic morphometry during aging in the dentate gyrus of ApoE knockout and human ApoE transgenic mice
… and display the highest syn/nrn and glutamate immunogold labelling in presynaptic boutons and dendrites of the MML, compared to hApoE3 and hApoE4 mice. In AD patients, such effects of the ?2 allele may act as a synaptic 'reservoir' and delay the onset of AD symptoms. Thus these lines of hApoE …
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Effect of metabolic insult on amino acids in the ciliary epithelium, lens, and retina
… approach, using quantitative silver-intensified immunogold detection of a range of immunoglobulins, can provide a metabolic profile of cells (metabolome). Despite the known alterations in the ciliary epithelium and lens function secondary to ocular ischaemia, little is know about the metabolic …
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Studies of intraorganelle dynamics: the lysosome, the pre-lysosomal compartment, and the golgi apparatus
… double immunofluorescent staining and double immunogold labeling of thin cryosections. Both lysosomal content and membrane protein intermixing were inhibited by nocodazole, a microtubule disruptor. In fused cells, lysosomes remained small, punctate and scattered throughout the cytoplasm. In …
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A biochemical and physiological characterization of coenzyme F420-reducing hydrogenase from Methanobacterium formicicum
… to be located at the cytoplasmic membrane using immunogold labeling of thin sectioned, Lowicryl-embedded cells. Neither enzyme was released from whole cells by osmotic shock treatment.
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Markov random field modeling of the spatial distribution of proteins on cell membranes
… away from receptors. Electron microscopy of immunogold-labeled membranes is a critical technique to generate topographical information at the 5-10 nm resolution needed to understand how signaling complexes assemble and function. However, due to experimental limitations, only two molecular …
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THE SPECIAL WALLS AROUND GAMETES IN CERATOPTERIS RICHARDII AND AULACOMNIUM PALUSTRE: USING IMMUNOCYTOCHEMISTRY TO EXPOSE STRUCTURE, FUNCTION, AND DEVELOPMENT
… through cell signaling via Ca+2 oscillations. Immunogold localizations revealed that AGPs are differentially expressed in the egg envelope in C. richardii. These glycoproteins are extremely abundant prior to fertilization but decrease substantially after fusion of the male gamete with the egg …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… in virus assembly and budding. Studies employing immunogold electron microscopy indicated that GP-C complex clusters into microdomains of 120 nm size independent of other viral proteins. Clustering of GP-C into membrane microdomains is neither influenced by SSP myristoylation nor by the …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
… in virus assembly and budding. Studies employing immunogold electron microscopy indicated that GP-C complex clusters into microdomains of 120 nm size independent of other viral proteins. Clustering of GP-C into membrane microdomains is neither influenced by SSP myristoylation nor by the …
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Immunocytochemical light microscopy and transmission electron microscopy study of growth hormone secreting cells in the porcine anterior pituitary
… partly empty vesicles than did control cells. Immunogold pGH antibody labeling showed that empty vesicles were devoid of electron dense particles. There was no significant difference in total number of vesicles between the controls and stimulated. Our current investigations reveal (1) a …
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