Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"immunofluorescent staining"”.
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Isolation and characterization of dengue virus membrane-associated replication complexes from Aedes aegypti
… from RNAi. Membrane isolation techniques and immunofluorescent staining techniques for dsRNA identification were developed to isolate and characterize membrane-associated replication complexes in DENV-infected mosquito cell culture and Ae. aegypti. Here we show that double-membrane vesicles …
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Investigation of the role of Glil+ cells in retinal angiogenesis and fibrosis.
… Eyes, and other organs, were collected for immunofluorescent staining. For the one laser choroidal neovascularisation (CNV) model, Gli1-Cre x Tomato mice received 3-4 laser burns per eye to induce choroidal neovascularisation and were monitored over 21 days to assess the expression of Gli1 …
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CD146 expression in early and late onset pre-eclampsia : is there a difference?
… in expression of CD146 immunohistochemical staining on intermediate trophoblast in early and late onset pre-eclampsia placentas as well as comparing this expression to gestational age matched control placentas. Study Design: Retrospective case series of 100 placentas: 25 early onset and 25 …
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Probing Mitotic Chromosome Structure and Arrangement Using Micromanipulation
… are microsurgically removed from cells. Immunofluorescent staining with tubulin antiserum reveals that the spindle is at the center of the extracted array and holds it together. When cells are treated with spindle poisons, the mitotic chromosomes are not radially arranged and chromosomes …
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The recombinant expression and localization of TvCP2 of trichomonas vaginalis
… TvCP2, in detail through Western blot analysis, immunofluorescent staining, and recombinant expression. The experiments 5 presented here suggest that aT l-CP2 over-expressing transfectant line processes CP2 and sequesters it in cellular compartments. Previous data gives strong evidence of the …
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MRGX1-TRPAI in inflammatory pain signalling
… well as an ex-vivo model of trigeminal nerves. Immunofluorescent staining revealed MRGX1 expression ex-vivo in dental afferents, which was more intense in inflamed versus healthy dental pulps. Endogenous MRGX1 protein expression was confirmed in both in-vitro models of human nociceptors. The …
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Modulation of calcium signalling in human sperm by nitric oxide
… in the regulation of human sperm functions. Immunofluorescent staining revealed constitutive NOS in human cumulus. DAF-FM diacetate staining demonstrated NO production by cumulus cells. Human sperm exposure to NO donors caused mobilization of stored Ca\(^{2+}\) by a mechanism not requiring …
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The effect of brain-specific VEGF-C deletion on vascular morphology in the brain and skull
… We studied VEGF-C expression by β-galactosidase staining in VegfcLacZ/+ mice. This revealed Vegfc expression in various parts of the brain. To investigate the role of VEGF-C in the brain, we generated Nestin-Cre; Vegfcfl/fl mice, in which we confirmed deletion of Vegfc. Nestin-Cre is expressed in …
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Antigenic Relationship of Different Porcine Rotavirus Isolates and the Identification of a New Porcine Strain
… rotavirus by positive fluorescence obtained by immunofluorescent staining of inoculated cell cultures. The antigens were used to produce high titer hyper immune sera in guinea pigs. These sera were used in appropriate serological studies in vitro to determine the amount of antigenic …
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Colony Stimulating Factor-1 Receptor (CSF-1R) Expression, Degradation, and Signaling in Lipid-Laden Macrophages
… for each experiment and used for comparison. Immunofluorescent staining was used to visualize and quantify CSF-1R, pAkt, pERK, pCSF-1R, and Ki67. To synchronize the signaling process, macrophages were deprived of CSF-1 supplemented media before experiments. The lipid-laden macrophages had a …
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Effect of phosphorylcholine-based small molecule analogues of the immunomodulatory nematode product ES-62 on mast cell function
… bound IgE or ionomycin/PMA. Finally, immunofluorescent staining showed that SMAs 63 and 64 and in addition 39, induced the global degradation of PKC-α from both the cytoplasm and the plasma membrane. It can be assumed from these date, that these SMAs particularly 63 and 64 could be …
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DNA repair deficiencies as a biomarker for treatment response in acute myeloid leukaemia
… genes which may be driving DDRD positivity. Immunofluorescent staining of DNA repair genes showed a clear repair deficiency in the DDRD positive cell lines, as the assay had predicted. Responses of the model cell lines to a range of chemotherapeutic agents in clonogenic assays can also be …
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Assessment of Immune System Involvement in Tourette Syndrome
… and 14 non-family control) were studied. Immunofluorescent studies were performed on basal ganglia tissue using serum from TS children and their family members. Brain antigens were prepared as two fractions (supernatant and pellet) and used as targets for ELISA. The pellet from putamen was …
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Direct Reprogramming of Fibroblasts into Smooth Muscle Cells
… SM22 and Calponin at mRNA and protein levels. Immunofluorescent staining of PiPS-SMCs showed positive staining for the above markers, demonstrating a typical SMC morphology. These cells displayed a greater potential to differentiate into SMCs than iPS cells. In order to elucidate the mechanism …
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Phenotypic Analysis and Localization of Leukocytes at the Site of Intradermal Injection of Phytohemagglutinin in Chickens
… At each time-point, one GF was used for immunofluorescent staining of pulp cell suspensions and leukocyte population analysis by flow cytometry, and another GF for histological analysis (Study I). Both studies revealed predominant infiltration of CD4+ T cells and αβ T cells in …
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Studies of intraorganelle dynamics: the lysosome, the pre-lysosomal compartment, and the golgi apparatus
… Co-localization was assessed by both double immunofluorescent staining and double immunogold labeling of thin cryosections. Both lysosomal content and membrane protein intermixing were inhibited by nocodazole, a microtubule disruptor. In fused cells, lysosomes remained small, punctate and …
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De-Risking PET Radioligand Discovery for Neurodegenerative Diseases
… of specific binding with aggregate TDP-43 immunostaining was evaluated for isotopologues of 6 existing tau PET radioligands in varying structural classes. Three radioligands, [3H]MK-6240, [3H]GTP-1, and [3H]JNJ-067, showed no binding in ALS tissues. [3H]CBD-2115 specific binding was elevated but …
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T-cell responses to human papillomavirus in men with anal high-grade squamous intraepithelial lesions
… Using whole slide imaging (600x) and two-colour immunofluorescent staining, CD4+ and CD8+ T-cell densities were measured. Lymphoid aggregates were CD4+ T-cell enriched (2.8-fold higher in density, P<0.01) compared to CD8+ T-cells (1.7-fold higher, P=0.08). Biopsies with HSIL diagnosis and having …
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Embryo and Gamete Development Upon Exposure to CCA Components: CrO3, CuO, and AS2O5
… patterns in MI and MII oocytes were analyzed by immunofluorescent staining. A sperm motility index was used to evaluate sperm function in the presence of CCA components. Sperm viability was analyzed using fluorescent staining.</p> <p>Summarizing our results, embryonic exposure to arsenic, …
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Innate Immunity in Chickens: In Vivo Responses to Different Pathogen Associated Molecular Patterns
… and at the local injection-site (pulp of GF; immunofluorescent staining of pulp cell suspensions). Intradermal injection of LPS or PGN in the pulp of GF resulted in increased heterophil levels in the blood and local tissue as well as smaller increases in macrophage proportions in the tissue. …
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