Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"immunofluorescence staining"”.
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
… of rhZP3 was detected by hemizona assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = …
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Evolution des programmierten Zelltods
… generated antibodies against these caspases. By immunofluorescence staining and cell fractionation we demonstrated that both caspases are localized in mitochondria in vivo.Caspase 3A is a constitutively active enzyme independent of PCD. After induction of apoptosis caspase 3B translocates from …
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Targeting c-Met for therapy
… were characterised by Western blotting, immunofluorescence staining, functional assays (ERK phosphorylation and cell scatter) and for their ability to recognise native c-Met by flow cytometry. Some of the anti-a-chain c-Met antibodies perform better in Western blotting and …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… histology, Ussing chamber analysis, and immunofluorescence staining of differentiation factors. I also found that this conditional reprogramming method permits cloning of human bronchial epithelial cells, and that these cells can support genetic modification by CRISPR. Next, I developed a …
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HEPATIC DIFFERENTIATION OF HUMAN AMNIOTIC EPITHELIAL CELLS AND IN VIVO THERAPEUTIC EFFECT ON ANIMAL MODEL OF CIRRHOSIS
… ACTIVITIES AND LIVER TRANSCRIPTION FACTORS. IMMUNOFLUORESCENCE STAINING SHOWED POSITIVITY FOR LIVER SPECIFIC MARKERS. FUNCTIONALLY, THESE CELLS SECRETED ALBUMIN, EXHIBITED INDUCIBLE CYP2C9 AND CYP3A4 ENZYMATIC ACTIVITIES, WERE ABLE TO UPTAKE AND CLEAR INDOCYANINE GREEN, AND UPTAKE LOW-DENSITY …
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The role of autophagy in allergic inflammation: a new target for severe asthma
… microscopy 8 10. Atg5 knockdown 8 11. Double immunofluorescence staining 10 12. Data analysis 10 III.RESULTS 12 1. The establishment of severe asthma model 12 2. The level ofLC3 expression in severe asthma model 15 3. Electron microscopy results 16 4. Inhibition of autophagy improved AHR and …
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Relative level of glucocorticoid and progesterone receptor: implications for immune and reproductive functions in women
… of primary antibodies by western blot analysis, immunofluorescence staining and flow cytometry. FGT ectocervical tissue explants were used as a model for the FGT. These were stained by immunofluorescence for GR and PR as well as for CD4+ and CD8+ markers. Immunofluorescence staining in …
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Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system
… with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon …
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Developing a selective sphingosine-1-phosphate-5 (s1p5) radiotracer to image oligodendrocytes using preclinical positron emission tomography (PET)
… For this, in situ hybridisation and immunofluorescence staining techniques were applied on adult naïve rat brain tissue sections. S1P5 was stained alongside various markers of oligodendrocyte developmental stage as well as markers for other central nervous system (CNS) cell types …
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Liquid-on-liquid mixing for slide-based biological assays
… technique increases the speed and sensitivity of immunofluorescence staining of Drosophila larval polytene chromosomes by a factor of 100 in time and concentration, when compared to standard coverslip techniques. Flow visualization experiments reveal the fluid motions in the thin aqueous layer. …
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Role of Nucleosome Remodeling Factor (NURF) in Tumorigenesis Using a Breast Cancer Mouse Model
… lab using 66cl4 breast cancer cell lines. Using immunofluorescence staining, no significant difference in CD8+, CD4+, NK and MDSC cells infiltrations into the tumor microenvironment was observed in 66cl4 tumors. Preliminary results from 67NR tumors suggested more CD4+ and CD8+ cells. Gene …
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Doxorubicin-Induced Cardiotoxicity Is Mediated By Neutrophils Through Release of Neutrophil Elastase
… Here, using echocardiography, flow cytometry and immunofluorescence staining, we demonstrated increased infiltration of neutrophils that correlated with decreased heart function (as defined by a decrease in ejection fraction and fractional shortening), disruption of vascular structures and …
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Defining a Novel Role for the E3 Ubiquitin Ligase Wwp1 in Cardiac Arrhythmogenesis
… detected by echocardiography. Furthermore, multi-immunofluorescence staining of cardiac tissue derived from the Wwp1 overexpressers at different stages of postnatal heart development showed mislocalization of the gap junction proteins Cx43 and Cx45. However, further analysis of these Cx by western …
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Hdac6 Inhibition Reverses Long-Term Doxorubicin-Induced Cognitive Dysfunction By Restoring Microglia Homeostasis
… in the cingulate cortex using Black Gold II staining, or changes in astrocyte reactivity as assessed by anti-GFAP immunofluorescence staining. Using advanced imaging techniques and single-nucleus RNA sequencing, I demonstrate that doxorubicin-induced changes are associated with decreased …
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Identification of a Novel ERK 1/2-Interacting A-Kinase Anchoring Protein
… a single primary cilium but not motile cilia. Immunofluorescence staining of primary cilia-containing cells indicates that RSP3/RSP3H localize to nuclear punctae, specifically promyelocytic leukemia (PML) bodies, suggesting a non-cilia related role for RSP3/RSP3H in these cells. Functionally, …
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TDI 천식 기도염증반응과 관련된 활성산소의 역할
… 15 3. Preparation of FITC-DBP conjugate and immunofluorescence staining 16 4.Flow cytometry analysis 16 5. Western blot analysis 17 6. Measurement of 1,25(OH)2D3 production and VEGF secretion 17 7. Statistical Analyses 18 III. RESULTS 19 A. Results 19 1. TDI-HSA conjugate increased tTG …
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Intracellular localization, biochemical and biophysical properties of human Armet
… through a flexible linker region. In this study, immunofluorescence staining was used to verify Armet’s localization in ER and Golgi apparatus in MBA-MD-231 cells. Evidence for calcium binding by Armet was obtained by circular dichroism spectroscopy (the binding of calcium appeared to decrease …
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THE ROLE OF ACTIVE AND ACTIVE-SITE ALKYLATED HUMAN O6-METHYLGUANINE-DNA METHYTRANSFERASE (MGMT AND R-MGMT) IN DNA REPAIR AND BIOMONITORING
… in the nucleus (except the nucleolus) by immunofluorescence staining using polyclonal and monoclonal antibodies. The supporting experiments came from differential staining of the GMT-deficient (mer-) and proficient (mer+) cells, Western blotting analysis, and specific antibody depletion …
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Polo-Like Kinase 1 and Smooth Muscle Cells in Coronary Artery Bypass Conduits and Coronary Arteries Following Angioplasty
… attenuated the expression of p-Histone H3. Immunofluorescence staining demonstrated an increase in the number of cells showing immunopositivity to PLK1, pPLK1, p-Histone, IFN-γ and pSTAT-3 in the neointima in post-PTCA coronary arteries and superficial epigastric vein (SEV) …
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Assessing the Photoprotective Effects of Fluorescent Sphingomyelin Against UVB Induced DNA Damage in Human Keratinocytes
… potentially streamlines future experiments. Two immunofluorescence stains for endogenous SM (lysenin) and ceramide were also optimized for preliminary fluorescence distribution studies and colocalization with FSM. Finally, a 3T3 fibroblast spheroid model was utilized as proof-of-concept for …
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