Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"immunofluorescence microscopy"”.
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Defining the functional role of MAGE-C1 in Multiple Myeloma
… MAGE-C1 interaction with RBX1 or SKIP, and immunofluorescence microscopy, to assess co-localisation of these proteins. Lastly, cellular pathways influenced by MAGE-C2 were evaluated using heat shock to induce a stress response and activate the pathways of interest. The expression of MAGE-C1, …
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Glucose metabolism in the outer segments of photoreceptor cells : its involvement in the phototransduction process
… by enzyme activity assays, Western blotting, and immunofluorescence microscopy. Activities of six glycolytic enzymes including hexokinase, phosphofructokinase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, pyruvate kinase, and lactate dehydrogenase, were found to be …
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Regulation of hnRNP A1 Cellular Localization by Protein Kinases and its Biological Impact
… role of HK2 in hnRNP A1 cellular localization by immunofluorescence microscopy, by analysis of HRV infection and by siRNA-based screening. These studies show that decreased HK2 protein levels lead to decreased cytoplasmic accumulation of hnRNPA1 during osmotic shock and HRV infection, to a …
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Lyme disease in Indiana : isolation of Borrelia burgdorferi-like organisms from Dermacentor variabilis
… of Lyme disease (LD), using dark-field and immunofluorescence microscopy, and tissue samples cultured in BSK II medium for 7 to 21 days. Spirochetes were isolated in the BSK II medium from pools of midgut diverticula from 6 D. variabilis from Martin County and pools of midgut diverticula …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… primarily at 24 and 48 hours post-infection by immunofluorescence microscopy. A domain in the N-terminal portion of the protein containing a variant MYND-binding motif was identified as the region responsible for TRP47 nuclear localization using ectopically expressed GFP-tagged TRP47 truncation …
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Some aspects of biochemistry of fibrous proteins in brain
… Cellular location of the changes was shown by immunofluorescence microscopy. This work was extended to include an investigation of a small amount of human material. Using the same analytical techniques as were employed in the experimental work on rats, a tentative identification is made of the …
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Surface Directed Monoclonal Antibodies against STEC Aid in the Reduction of Pathogen Detection Times from Food and Water
… Dynabeads, and detection via fluorescence microscopy, (2) the addition of enrichment culture into a flow through system consisting of a column packed with large polystyrene beads (≥ 100 μm) coated with specific mAbs for capture. The results indicate that the filtration approach can only be …
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Discovery and characterization of pentose-specific transporters in Saccharomyces cerevisiae
… S. cerevisiae as indicated by HPLC analysis and immunofluorescence microscopy, respectively. Kinetic parameters of the transporters were determined using a 14C-labeled sugar uptake assay. Sugar uptake assay in un-buffered cell suspension indicated the sugar uptake through these three transporters …
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Eukaryotic protein mimicry as an infection strategy for Ehrlichia chaffeensis
… family of receptors. In this study, we used immunofluorescence microscopy to demonstrate very strong colocalization between E. ch. and Fzd2, 4, 5, 7, and 9, as well as coreceptor LRP5 1-3 h post infection. Direct binding between TRP120 and multiple Fzd receptors was further confirmed by ELISA …
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Inhibition of the endosomal recycling pathway to overcome resistance to cancer therapies
… We used Western blot, quantitative RT-PCR, and immunofluorescence microscopy to confirm the RPPA results. We found that another endosomal recycling inhibitor, monensin, also potently downregulates these hormone receptors and that both inhibitors synergise with tamoxifen and enzalutamide, …
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Major Sclerotial Polypeptides Of Psychrophilic Fungi: Identification, Immunological Relatedness, Localization And In Vivo Synthesis
… sclerotial polypeptides in the sclerotia by immunofluorescence microscopy showed that the major polypeptides were sequestered in sclerotial protein bodies in all four species. This indicates that the major sclerotial polypeptides may play a role as storage proteins. Major sclerotial …
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Studying Tissue Specific Diversity of Splicing Regulator Muscleblind
… isoforms have not been fully explored. Using immunofluorescence microscopy and polyclonal serum, we analyzed Mbl protein expression across adult Drosophila tissues. Mbl was detected in various locations, including the brain, gonads, muscle, and gut epithelium. Skeletal muscles demonstrated the …
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Ryanodine receptor expression in trophoblasts
… was also revealed in BeWo and JEG-3 cells by immunofluorescence microscopy. In addition, changes in [Ca2+]i were observed in both BeWo and JEG-3 cells upon application of various RyR agonists and antagonists, using fura-2 fluorescent videomicroscopy. Furthermore, endogenous placental peptide …
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Single and hybrid antimalarials based on artemisinin, chloroquine and ß-lactams : synthesis, antiplasmodial activity, cytotoxicity and effect of selected artemisinin-chloroquine hybrids on the parasitic endocytosis pathway
… well as localization of transport vesicles via immunofluorescence microscopy were determined. A series of β-lactam derivatives containing a terminal acetylene moiety were synthesized via the Staudinger and Ugi 3-component 4-centre condensation reactions. The compound with the best activity from …
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INTRODUCTION OF A NOVEL HOME-BASED HIGH-INTENSITY INTERVAL TRAINING PROGRAMME TO IMPROVE CARDIO-METABOLIC HEALTH IN AT-RISK INDIVIDUALS
… and GLUT4 protein expression using quantitative immunofluorescence microscopy. Adherence and compliance (Home-HIT 96±3% & 99±1%; Home-MICT 88±4% & 100±0%; Lab-HIT 97±1% & 100±0%, respectively) to training did not differ between groups. Training increased V ̇O2peak and Matsuda insulin sensitivity …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… were captured in G₁. Using quantitative confocal immunofluorescence microscopy and fluorometric ELISA, we show that G₀ fibroblasts were significantly hypomethylated at lysines (K) of histone 3 (H3), specifically H3K4me3, H3K9me2, H3K9me3 and H3K27me3, but not H3K9me1. Histone acetylation was …
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WIP1 Phage Specificity for B. Anthracis
… antibody neutralization assays. From indirect immunofluorescence microscopy, we also demonstrated that Wip1 p23 binds very specifically to the surface of B. anthracis. Finally, we characterized the bacterial receptor for Wip1 as a Sap-dependent cell wall component. The work in this dissertation …
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Interactions Between Host Cells And Merkel Cell Polyomavirus In The Context Of Infection And In Merkel Cell Carcinoma
… methods including qPCR, western blotting, immunofluorescence microscopy, qPCR, and lentiviral CRISPR knockout. We found that MCPyV infection leads to innate inflammatory cytokine induction in response to late events in the viral infectious cycle. This innate gene induction was mediated by …
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Spatial patterns of wheat grain composition in relation to nutritional quality and processing properties
… chemistry, immunocytochemistry and microscopy, have been used to achieve the research aims. The results show a clear deceasing gradient in total protein in the endosperm from the subaleurone toward the centre of the grain. Strong spatial gradients were also observed in the …
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Evaluating convolutional neural networks and transformer architectures for image-based prediction of protein localization in eukaryotic cells
… to tackle this problem by leveraging large-scale immunofluorescence microscopy datasets. The aim of this study is to comparatively evaluate convolutional neural network (CNN) architectures and Transformer- based models for the multi-label classification of protein subcellular localization in …
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