Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"immunodetection"”.
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The immunodetection of cocaine for forensic applications.
… significant concentrations. Secondly, a novel immunodetection method was developed for the location-specific staining of cocaine on banknotes. The method was based on the application of an acrylamide gel matrix for maintaining the position of the cocaine during subsequent immunostaining. The …
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Effect of irradiation of head and neck tumours on the proteins' composition of the enamel salivary pellicle
… separation and silver-staining as well as immunodetection of alpha-amylase, IgA and acidic-PRPs. Conclusively to our results, irradiation of the head and neck region seems to affect the quantity but not the chemical composition of the enamel salivary pellicle.
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Early Mediators of Cutaneous Ischemia Reperfusion Injury: A Mouse Model
… concentrations found at day 1. Simultaneously, immunodetection of heme oxygenase was performed, revealing high levels of inducible HO-I throughout the time course, with no change in HO-2 expression. Tissue damage was confirmed through histological examination.
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Immunomodulation der Kartoffel-Blattrollvirus (PLRV) Multiplikation via Expression virusspezifischer rekombinanter Antikörper in planta
… antibody mAbP1-C25 and could be used for immunodetection of recombinant and native P1 and P1-C25. In a transient expression approach co-transformation of an infectious PLRV cDNA clone and the antibody scFvP1-C25 in Nicotiana tabacum strongly reduces virus accumulation. Transgenic potato …
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The Effect of High Salt Intake on Betaine-Homocysteine S-Methyltransferase Expression in the Guinea Pig
… have been shown to express BHMT in the kidney. Immunodetection methods were used to demonstrate that humans and guinea pigs have similar patterns of hepatic and renal BHMT expression and suggest that the guinea pig is a suitable in vivo model for studying renal BHMT. Guinea pig, however, also …
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An investigation into the specific function of the vaccinia virus :13.8 kDa protein encoded by the N1L gene
… expression system and positively identified by immunodetection studies.
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An investigation into the specific function of the vaccinia virus 13.8 kDa protein encoded by the N1
… expression system and positively identified by immunodetection studies.
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Forensic taphonomy: investigating the post mortem biochemical properties of cartilage and fungal succession as potential forensic tools
… forming heterogeneous subpopulations with time. Immunodetection of aggrecan ceases when joint exposure to the soil environment occurs. At this time, aggrecan is metabolised by soil microbes. The molecular breakdown of cartilage proteoglycans has potential for use as a reliable indicator of PMI, …
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Épissage alternatif et mutations ponctuelles de Pax5 chez la leucémie lymphoîde chronique et les lymphomes de cellules B
… method of analyses of PCR products and immunodetection techniques, we have discovered that multiple isoforms of Pax5 are simultaneously expressed in normal B lymphocytes, as well as in B lymphocytes isolated from lymphoma and CLL patients. Further, we believe we have identified a …
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Expression of Usherin in Human Tissues: Implications for Diagnostics and Pathology
… as controls. These results, in conjunction with immunodetection of usherin, could lead to diagnostic procedures for th& detection of Usher Syndrome Type 11a and carriers of an USH2A alleie. The presence of usherin in the reproductive tissues prompted the investigation of reproductive patterns …
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Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… expansion was further evidenced by increased immunodetection of lysosomal associated membrane protein-1 (Lamp-1) and Lysotracker-based flow cytometric analysis. While lysosomal membrane integrity remained intact, autophagic blockade was suggested by accumulation of cellular protein levels of …
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Detection and analysis of the wood decay fungus <i>Lentinus Lepideus </i>Fr. using immunological probes
… of immunological techniques to (i) develop an immunodetection system for the fungus and (ii) to analyse L. lepideus antigens. Preliminary experiments indicated that immunological techniques could be used to detect the fungus. Nitrocellulose-based systems proved to be the most sensitive and …
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Synthesis of a Diverse and Three-Dimensional Fragment Collection and The Development of a Novel Platform for Antibody Dual Functionalisation
… of antibodies for use in both drug delivery and immunodetection has gained widespread interest in recent years, particularly in the field of oncology. Although many strategies have been developed to enable such modifications, the majority only enable the incorporation of a single type of payload, …
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The molecular characterisation of pregnancy-associated plasma protein-A (PAPP-A).
… were abolished by performing Western blotting immunodetection at a high salt concentration (0.6M NaCl). At this salt concentration a single band of 195 kDa was immunodetected and this corresponded to the monomeric PAPP-A molecule. It was also discovered that a subset of paratopes in this …
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Correlation between the expression of integrins and their role in cancer progression. Expression pattern of integrins αvβ3, αvβ5 and α5β1 in clinical and experimental tumour samples
… cell lines was subsequently evaluated using immunodetection methods in cells and xenograft tumour samples. The effect of integrin inhibition on cell migration was then assessed using neutralizing antibodies against αvβ3, αvβ5, and α5β1 integrins in the scratch-wound healing assay. This assay …
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Angiotensin-converting enzyme cleavage of the Alzheimer's beta-amyloid peptide
… Aβ(1-16) and Aβ(1-42) was investigated using immunodetection and mass spectrometry. Similar to the ACE inhibitor lisinopril, the Aβ peptides elicited ACE signalling by phosphorylation of the cytoplasmic Ser1270 residue and JNK activation. The signalling response of ACE was coupled to increased …
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Cellular pathways of angiotensin II signalling in the hypertrophic myocardium
… subunits were assessed by ADP ribosylation, and immunodetection of Gs alpha. A diminution in the concentrations of these proteins in the hypertrophic Dahl S ventricular myocytes in comparison to Dahl R ventricular myocytes, was observed. Furthermore, the reversal of cAMP inhibition by ATII was …
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Dynamic organization of chromosomes in the mammalian cell nucleus
… mid-to-late replicating chromatin. Simultaneous immunodetection of lamin B on two-color replication labeled neuroblastoma cell nuclei revealed specific attachment of the mid-to-late replicating chromatin compartment not only along the periphery but also inside the nucleus along invaginations of …
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Metabolismus und Biomineralisation in anaerob Methan-oxidierenden Lebensgemeinschaften
Die anaerobe Oxidation von Methan (AOM) ist als Schlüsselprozess des marinen Methankonsums bekannt. Nach phylogenetischen und metagenomischen Analysen wird der Prozess von Konsortien aus syntrophen Archaeen und Sulfat reduzierenden Bakterien katalysiert. In dieser Arbeit wird ein umfassenderer …