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Showing 1 to 5 of 5 for “"immunodepletion"”.

  1. Isolation of Mesenchymal Stem Cells Derived from Adult Bone Marrow and Umbilical Cord Blood and Their Potential to Differentiate into Osteoblasts

    … blood using a novel isolation method based on immunodepletion. Furthermore we will look at the potential of these isolated MSCs to differentiate into mature, bone producing osteoblasts. The results of the studies showed that our novel isolation method allowed proliferation of a homogeneous MSC …

    vcu Repository record for Isolation of Mesenchymal Stem Cells Derived from Adult Bone Marrow and Umbilical Cord Blood and Their Potential to Differentiate into Osteoblasts (opens in a new tab)

  2. Induction and Regulation of CXCL10 in Hepatocytes During Hepatitis C Virus Infection

    … cultures contain non-parenchymal cells (NPCs). Immunodepletion of NPCs eliminated expression of immune and anti- inflammatory markers in PHH cultures, which then showed no IFN requirement for CXCL10 induction during HCV infection. Instead, HCV infection and specific TLR3/RIG-I activation induced …

    washington Repository record for Induction and Regulation of CXCL10 in Hepatocytes During Hepatitis C Virus Infection (opens in a new tab)

  3. The Role of the Protein MTBP in DNA Replication

    … independently of licensing and CDK/DDK activity. Immunodepletion of MTBP from extract leads to co- depletion of Treslin and causes a strong inhibition of DNA replication and impairment of CMG assembly. A partially purified fraction of extract, enriched for MTBP and Treslin, can rescue DNA …

    dundee Repository record for The Role of the Protein MTBP in DNA Replication (opens in a new tab)

  4. Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation

    … that is dependent on the SUMO E3 ligase, PIASy. Immunodepletion of Vespera from Xenopus egg extracts interferes with proper nuclear formation and nuclear transport, and rescue of this defect requires Vespera SUMOylation. Addition of excess amounts of Vespera to metaphase extracts disassembles …

    rockefeller Repository record for Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation (opens in a new tab)

  5. The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint

    … were raised and used to establish the effective immunodepletion of Pin1 from extracts of Xenopus laevis eggs. While the SAC could readily be activated in mock-treated samples of this cell free system, securin was degraded despite the presence of unattached kinetochores when Pin1 had previously …

    bayreuth Repository record for The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint (opens in a new tab)