Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 182 for “"immunoblotting"”.
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Identification and Partial Characterization of the Antigens of Anaplasma Marginale (Florida) and Anaplasma Caudatum (Illinois) (Immunoblotting, Hemoparasite, Anaplasmasis)
Shared and unique antigens were identified and partially characterized for two morphologically and antigenically distinct isolates of Anaplasma, A. marginale (Florida) (FAM) and A. caudatum (Illinois) (IAC). Crude CA-like antigens were prepared from the parasitemic blood of each calf, separated by …
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Αντισώματα κατά Ro(SSA) και La(SSB) κυτταρικών αντιγόνων: Ανάπτυξη μεθόδου ανοσοαποτύπωσης (Western blot)
An immunoblotting method for the detection of antiRo and antiLa antibodies was developed, using two antigenic sources, thymus extract and human red blood cells. The reactivity of sera from patients with primary Sjogren’s syndrome towards the Ro and La polypeptides was examined and compared with the …
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Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
… between maize and sorghum β-glucosidase, immunoblotting analysis was performed with maize-anti-β-glucosidase serum. The immunoblotting results supported the results of Hosel et al., (1987) with respect to the occurrence of two distinct β-glucosidases being present in sorghum.
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Expression of Bacillus Anthracis Protective Antigen in Vaccine Strain Brucella Abortus Rb51
… (pBBR1MCS) and shown to express in E. coli by immunoblotting using antiserum specific for PA. The immunoblot results revealed that E. coli produced a PA protein of the expected size. In addition, the culture medium was shown to contain the same PA protein using immunoblotting. These results …
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Enhancement of plant expression vectors using replication and silencing suppressor elements
… the silencing suppression were determined using immunoblotting and fluorescence of EGFP. In addition, replication elements from three viruses (bean yellow dwarf virus [BeYDV], beak and feather disease virus [BFDV] and begomovirus alphasatellite) were assembled into novel plant expression vectors …
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Investigating The Effects of Silencing Epha2 In Metastatic Breast Cancer Cells
… lines was screened for expression of EphA2 by immunoblotting. Several of the overexpressing cell lines, including BT549, MDA-MB-231, and HCC 1954 were selected for experiments utilizing siRNA for transient knockdown and shRNA for stable knockdown. Targeted knockdown of EphA2 was measured using …
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Molecular characterisation of parvalbumin and analysis of cross-reactivity in five fish species using sera from fish-allergic consumers and occupationally exposed workers
… fish species in South Africa were assessed by immunoblotting and the most cross-reactive species was characterised further. Pilchard parvalbumin was identified as the most cross-reactive allergen in fish-allergic consumers. The cDNA sequenceß form of pilchard parvalbumin was determined. This is …
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Molecular candidates for Blocking the Transmission of Vector-Borne diseases
… PCR to detect dengue serotypes DENV2/3 RNA and immunoblotting to detect viral proteins within mosquito extracellular vesicles (EVs). The EVs carry infectious viral RNA and are able to establish infection in naïve, recipient cells of various lineages. Mosquito EVs mediate DENV2 transmission …
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Analysis of TpeL secretion in Clostridium perfringens
… we able to detect PilT or FtsA overexpression by immunoblotting, and the project was discontinued in November 2017. In retrospect, these experiments likely failed because of an inactive promoter region in the overexpression plasmid. Though clostridial diseases require secreted toxins, their …
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Identification and Characterization of the TGFβ/Hippo Crosstalk through Mathematical Modeling
… of Smad/Taz/Yap proteins or receptors. Immunoblotting experiments revealed that Taz/Yap knock down alters phosphorylation/dephosphorylation of Smads. IF microscopy showed that there is no difference in the export and dephosphorylation rates in cells transfected with siCTL versus …
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Nitric oxide in the rat gastrointestinal tract
… the nature of NOS and small intestine. Immunoblotting was performed using polyclonal antisera raised against two peptides found in the rat brain NOS sequence and commercial monoclonal antibodies directed against neuronal and endothelial isoforms of NOS. A160kDa band was detected in brain …
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A Study of alpha-Synuclein, Parkin and TDP-43 ; Proteins Implicated in Neurodegenerative Disease.
… blood plasma. Immunoprecipitation followed by immunoblotting techniques were utilised to first capture and then assess the molecular weight of the complex. Ion exchange chromatography and gel-filtration chromatography allowed the complex to be further isolated and characterised. ELISA assays …
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An immunohistopathological and functional investigation of β3 integrin antagonism as a therapeutic strategy in cancer. Characterisation, development, and utilisation of preclinical cancer models to investigate novel ¿3 integrin anatgonists.
… subunits was detected in xenograft tissue using immunoblotting techniques. A panel of cell lines of different tumour types including melanoma, prostate, breast, colon and non small cell lung carcinoma was then characterised for αVβ3 and αIIbβ3 integrin expression using immunoblotting and …
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Chemical and physical properties of bovine alpha-lactalbumin, beta-casein and genetically altered bovine beta-casein produced in the milk of transgenic mice
… conditions. Using PAGE followed by immunoblotting, both heterologous proteins were proved as identical as the native proteins in the molecular weight and the charge. The estimated expression level of bovine $\alpha$-lactalbumin and $\beta$-casein was about 1.0 mg/ml and 3.0 mg/ml, …
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SUFU in SHH signalling mediated myogenesis
… of the pathway during this time. Alongside this, immunoblotting results show the absence of SUFU during proliferation of the cells and its presence throughout differentiation. These results postulate a role for SUFU as an inhibitor during Shh mediated myogenesis.
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Hibernation is super complex: dynamics of electron transport system supercomplexes
… using 2-dimensional gel electrophoresis and immunoblotting. Rats had fewer SCs than TLGS. SCs are dynamic in hibernation and the complex III composition of different SCs differed between hibernation states and seasons. There was no correlation between SC abundance and mitochondrial …
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Micromanaging Freeze Tolerance: The Biogenesis and Regulation of microRNAs in Frozen Frogs
… of microRNA in a freeze tolerant vertebrate. Immunoblotting was used to investigate protein abundance of key microRNA biogenesis factors in brain and liver of control, 24 h frozen, and 8 h thawed R. sylvatica. Biogenesis capacity was reduced in brains and elevated in livers during freezing and …
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Protein Turnover in Rat Skeletal Muscle During In Vitro Hypo-Osmotic Stress
… to determine protein synthesis and degradation. Immunoblotting was performed to assess signalling pathways involved. Phenylalanine uptake and incorporation were increased by 199% and 169% respectively in HYPO from ISO (p < 0.05). This was supported by elevated phosphorylation of mTOR Ser2448 …
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Identification of Peptidyl-prolyl cis/trans isomerases affecting Drosophila circadian rhythms
… and methods for S2 cell-based assays 8 E. Immunoblotting 8 F. Immunoprecipitation 9 G. Immunohistochemistry 10 H. Quantitative real-time RT–PCR 11 Ⅲ. RESULTS 12 A. PPIases alters Drosophila circadian activity rhythms 12 B. DOD 19 1. Daily cycle of core clock protein in dod knockdown …
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Learning and cemory in the cav1.2 knockout mouse
… extensive deletion was confirmed by RT-PCR and Immunoblotting. To test for spatial learning and memory, a series of Morris water maze experiments were performed. Knockout mice showed no deficits in short-term (24-hr) memory trials; however, on a 30-day memory probe, knockout mice performed …
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