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Showing 1 to 20 of 120 for “"immunoblot"”.
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IgE and IgG Binding Patterns to Dermatophoides Pteronyssinus and Dermatophagoides Farinae in Children with Atopic Dermatitis : An IgE Immunoblot Analysis
"-국문 요약 - 소아 아토피피부염 환자에서 Westernblot을 이용한 D. pteronyssinus와 D. farinae 조항원에 대한 IgE 및 IgG 항체 결합 양상 비교 목적: 집먼지진드기 항원에의 감작은 주로 4-5세 이상의 소아나 성인에서 의미 있게 관찰되나 3세 미만의 영 유아에서도 중요한 원인이 될 수 있다. 성인 환자들을 대상으로 시행한 외국의 연구에 의하면 140 kD-6 kD 사이의, 최소 32개의 IgE 결합 단백이 존재하며, 이 중 Group I(약 25 kD), Group II(약 15 kD), 및 …
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Identifizierung und Charakterisierung IgE- reaktiver Proteine in der Tomate (Lycopersicon esculentum)
… 18 tomatensensibiliesierten Patientenseren im Immunoblot getestet. Detektierte Proteine in der SDS-PAGE wurden mittels LC-MS/MS identifiziert. Dadurch konnten 2 Tomatensamenproteine, die im Immunoblot ein IgE- reaktives Signal zeigten eindeutig mittels Massenspektrometrie identifiziert werden. …
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The molecular and biochemical characterization of cell cycle regulators in Pisum sativum
… cycle regulators in Pisum sativum (garden pea). Immunoblot patterns indicate that $\sim$34 kDa cdc2-type proteins present in protein complexes may be phosphorylated in pea. As in other systems, these proteins copurify with a histone H1 kinase activity by p13$\sp{suc1}$-Sepharose affinity …
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Tissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsis
… antibody was produced using purified XCP1H6. Immunoblot analysis of a developmental time course of xylem and bark protein extracted from root-hypocotyl segments demonstrated that XCP1 was expressed in xylem only. Further analysis under optimized immunoblot conditions, however, revealed that …
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Immunological and Molecular Analyses of the Borrelia burgdorferi OspF Protein Family F
… OspF proteins during murine and human infection. Immunoblot analyses revealed a temporal expression of OspF proteins during infection and mapped the immunodominant epitopes which lie within the variable domains. To determine if OspF-related proteins are produced by other isolates, immunoblot …
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Development of confirmatory testing strategies for suspect false PRRS-positive reactors in diagnostic serology
… conducted first to develop immunoassays (Western immunoblot and microsphere immunoassay) based on recombinant N protein of PRRSV as well as bELISA using the existing commercial ELISA kit (2XR). Then diagnostic performances of four different serologic assays (Western immunoblot, microsphere …
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Novel Diagnostics and Therapeutic Approaches for Supratentorial Ependymoma
… selected through phage-display and tested in immunoblot and immunohistochemistry assays before lead immunoglobulins were subjected to affinity maturation via ribosome display. Affinity-matured candidates were assessed for their diagnostic potential in immunoblot analysis blotting, proteomic …
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Plant-based Multicomponent Vaccines against Enteric Diseases
… were detected in transformed tuber tissues by immunoblot assay and quantitated by enzyme linked immunosorbent assay (ELISA). The CTB-NSP4 protein expression level was found to be approximately 0.01% of total soluble plant protein (TSP) and the CFA/I-CTA2 protein expression level was about …
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Antikörper-Entwicklung nach Respiratory Syncytial Virus (RSV)-Infektion bei Frühgeborenen und Säuglingen
… die AK-Entwicklung nach RSV-Infektion mittels Immunoblot zur Auswertung. <br>In der Gruppe der Studie 1-Kinder (junge Säuglinge und Frühgeborene) wurden Frühgeburtlichkeit und familiäre Vorbelastung mit Asthma als Risikofaktoren für eine RSV-Infektion gemäß früherer Studien bestätigt, nicht …
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Diagnóstico e fatores de risco associados à cisticercose bovina em matadouros sob inspeção federal e estadual, localizados no município de Colatina-ES
… foram analisadas através das técnicas ELISA e immunoblot e os resultados foram organizados em tabela de contingência para cálculo do desempenho dos testes determinado pelas taxas de sensibilidade e especificidade, valores preditivos positivo e negativo e concordância. A partir da Guia de …
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Development and characterization of recombinant human autoantibody using cDNA made from peripheral blood mononuclear cells of patients with bronchial asthma
… gel electrophoresis (SDS-PAGE) 와 immunoblot 11 2. 정제된 scFv항체들을 이용한 ELISA 11 F. 인간 유전자 재조합 단클론 IgG 자가항체의 제작 및 정제 12 1. IgG 자가항체의 제작 12 2. IgG 자가항체 생산을 위한 세포배양 12 3. Protein A column을 이용한 IgG 자가항체의 정제 13 G. IgG 자가항체의 CK-18 단백질에 대한 반응 평가 13 1. IgG 자가항체를 이용한 항원 반응특성의 ELISA 13 2. …
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Mechanism-Based Strategies to Enhance The Actions of A
… levels were measured using real time RT-PCR and immunoblot assays. Treatment with 0.5 µM 17-AAG for 8 hours resulted in the induction of all HSP transcript and protein levels in the MM cell lines. This induction of HSP mRNA levels was diminished by 0.05 µg/mL Act D for 12 hours in the combination …
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Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
… 8 F. Co-immunoprecipitation and immunoblot analysis 8 G. Two-dimensional electrophoresis 9 H. Subcellular fractionation 10 I. Immunohistochemistry 10 J. Statistical analysis 11 Ⅲ. RESULTS 12 A. Localization of Prx III and Srx in A549 cells 12 B. Oxidation of Prx III in mink lung …
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Investigations of cellular stress-responsive proteins and PGF gene expression in human trophoblast
… placentae were assessed for PML expression. Immunoblot, qRT-PCR and immunohistochemistry techniques were used to determine PML gene expression and localization in placental tissue and primary cells. Results: Two consensus ARE motifs were detected within the human PGF 3'UTR at the 42nd …
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Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice
… 10 1. Preparation of PA 10 2. Cell culture 10 3. Immunoblot analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs 13 6. Uptake of 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino-2-deoxyglucose (2-NBDG) 14 7. Animal experiments 14 8. FGF21 secretion 15 …
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Untersuchung zur Spezifität von Autoantikörpern bei Patienten mit linearer IgA Dermatose
… gestationis. Tatsächlich zeigte sich im Immunoblot bei 11 von 50 LAD-Patienten eine Reaktivität von IgA-Antikörpern mit einer rekombinanten Form von BP180 NC16A. Wir fanden bezüglich Alter, Geschlecht und immunfluoreszenzoptischer Befunde keine signifikanten Unterschiede zwischen der …
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Caractérisation du STF-62247 comme un inhibiteur de PIKfyve dans les carcinomes rénaux à cellules claires
… de type Western. Cet écart de migration sur l'immunoblot a été prévenu par un traitement à la lambda phosphatase, suggérant ainsi que le STF-62247 engendre une phosphorylation de PIKfyve par un mécanisme qui nécessite encore plus d'investigation. Cette modification n'a pas été observée dans les …
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Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants
… was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a molecular mass of about 30 KDa and was approximately 0.01% of the total soluble protein in transgenic potato tissue.</p>
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The effects of aging on cardiac mechanics
… aging. Cellular protein changes were assessed by immunoblot (western) analyses in order to establish if material property changes also occurred with aging. The western results indicate significant (p<0.05) changes in cytoskeletal and mechanotransduction proteins with aging. These data support the …
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Type IV Pili-Dependent Secretion of Biofilm Matrix Material Proteins in Clostridium perfringens
… a CPE0515-FLAG construct, it was concluded from immunoblot densitometry data that, except for the pilA1 deletion mutant, none of the 18 tested pilin mutants had a statistically significant difference from the wild type (WT) with regard to protein secretion. From slot blot densitometry assays, it …
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