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Showing 1 to 20 of 31 for “"immunoaffinity"”.
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Selective cell separation utilizing porous immunoaffinity membranes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.
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A Capillary-Based Microfluidic System for Immunoaffinity Separations in Biological Matrices
… of analytes from complex and limited matrices. Immunoaffinity separations in miniaturized formats offer selective isolation of target analytes with minimal reagent consumption and reduced analysis times. A prototype capillary-based microfluidic system has been developed for immunoaffinity …
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Development of a novel monoclonal antibody-based immunoaffinity column for the detction of aflatoxins
… applications to date. -- In this study, a set of immunoaffinity columns were produced in order to achieve one main aim; to produce a diagnostic tool that has the ability to compete with others already established on the immunodiagnostic market, whilst making use of 1G7-1E2. The results provide …
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Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents
… these important therapeutics from complex media. Immunoaffinity chromatography has been an important method in the isolation of these products, typically being implemented as the final cleanup step yielding an extremely pure, homogenous final product. However, the use of immunoaffinity …
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Identification of the factors impacting immunosorbent performance
Immunoaffinity interactions can play a vital role in the purification of therapeutic proteins. In most cases immunoaffinity chromatography is used as the final clean up step to yield a highly pure product. The customized avidity of the parent antibody for a given antigen or hapten can make …
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Novel customisable phases for micro solid-phase extraction and automated biological sample preparation
… reactor (IMER) and a µSPE immunoaffinity cartridge to alleviate sample preparation bottlenecks caused by conventional ~18-hour digestions. A novel and customisable material was prepared for bio-ligand immobilisation. A hybrid inorganic-organic material using silica modified …
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A microfluidic platform for hematopoietic stem cell separation
… optimization. In this work, a microfluidic immunoaffinity based silicon micropost device for hematopoietic stem cell enrichment is discussed, which demonstrates how innovations in microfluidics can revolutionize current clinical therapies. Appropriate surface chemistry for robust …
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Bioanalytical methods for studies of homocysteine and novel cardiovascular disease indicators
… the feasibility of using hybrid organic-immunoaffinity extraction for GC-MS analysis of polycyclic aromatic hydrocarbons in human heart plaque samples. This body of work is significant because it proposes new bioanalytical technologies that could enhance CVD screening and treatment.
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The Role of Phosphorylation in Modulating PC4 Function as a General Cofactor
… RNA polymerase II are achieved by a one-step immunoaffinity purification. The purified complexes, which are devoid of other general transcription factors (GTFs) commonly copurified with TFIIH and RNA polymerase II by traditional approaches, are fully functional in mediating both basal and …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… assays could be verified by subtractive immunoaffinity chromatography of Nhe B and testing the Nhe B negative, HBL positive B. cereus culture supernatants (Nhe B-/HBL+). Furthermore it could be demonstrated for the first time that Nhe B plays a major part in the appearance of the …
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Development of Magnetic Nanoparticle-based Enrichment Techniques and Mass Spectrometry Methods for Quantification of the Clinical Biomarker Cardiac Troponin I
… and conjugated with antibodies to act as immunoaffinity carriers. Magnetic nanoparticles were selected due to their variable surface modifications, high binding capacity, and the fact that they can be easily isolated using a magnet. After optimizing the enrichment and digestion procedures, …
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Purification, serology and pathogenic role of the 110 kilodalton rtx hemolysins of Actinobacillus pleuropneumoniae
… and HlyII was purified from serotypes 1 and 5 by immunoaffinity chromatography. An indirect enzyme-linked immunosorbent assay (ELISA) using MAb 8C2, or affinitypurified rabbit IgG to both hemolysins, was developed for detection of swine antibody to one or both hemolysins, respectively. In …
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Purification and characterization of Clostridium sordellii toxins HT and LT and comparison to toxins A and B of Clostridium difficile
… through an XM-100 membrane filter and immunoaffinity chromatography using a monoclonal antibody to toxin A of C. difficile as the ligand. Toxin LT was purified to 80% homogeneity by ultrafiltration on an XM-100 membrane filter and ion-exchange chromatography. Toxin HT migrated as a …
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Genome-Wide Localization And Novel Deposition Pathways Of Histone Variant H3.3 In Embryonic Stem And Neuronal Precursor Cells
… of H3.3 at rDNA, telomeres, and specific TFBS. Immunoaffinity purification and mass spectrometry reveal that the proteins Atrx and Daxx associate with H3.3 in a Hira-independent manner. Using Atrxflox and Atrxnull mouse ES cells, I find that Atrx is specifically required for Hira-independent …
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Purification and Characterization of the Prostate Cancer-Associated Antigen: 7E11-C5
… 400-fold from LNCaP xenograft tissue extract by immunoaffinity chromatography with an estimated antigen recovery of 64%. High pressure liquid chromatography (HPLC) molecular exclusion column was used to further purify the antigen and results showed that the molecular weight of the antigen, under …
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Differential glycosylation of Tractin and LeechCAM, two novel Ig-superfamily members, regulates neurite extension and fascicle formation
… carrying the glycosylated epitopes;I have used immunoaffinity purification techniques with the Lan3-2 antibody to identify the molecules carrying the Lan3-2 and Lan4-2 glycoepitopes. Both epitopes are expressed on two novel Ig-superfamily members, Tractin and LeechCAM, respectively. Tractin has …
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A Characterization of the Effect of Apolipoprotein AII on the Structure and Function of Apolipoprotein AI in Reconstituted High Density Lipoproteins
… lipoproteins (HDL) can be fractionated by immunoaffinity chromatography into two main subclasses: a fraction which contains apolipoprotein (apo) AI and apoAII, and a fraction which contains apoAI but not apoAII. The effect of apoAII on the structure and function of apoAI in reconstituted …
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Optimization of large beaded cellulose as a chromatographic support
… cellulose supports derivatized to form either immunoaffinity or anion exchange matrices exhibited very low non-specific binding. Thus, the particle size, solids content, and extent of derivatization of cellulose matrices can be engineered so as to create matrices that provide high flow rates …
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Protein factors involved in the biogenesis of the mitochondrial ribosome
… is depleted in YbeY knockout cells. Further, immunoaffinity purification identified MRPS11 as a key interactor of YbeY. We propose that YbeY is a multifunctional protein that performs endonucleolytic functions in the mitochondria and also acts as a mitochondrial ribosome biogenesis factor, …
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Immunogenetic analysis of HLA Class II in premalignant disease of the cervix and correlation with HPV status
… and the risk for CIN after HPY infection. Immunoaffinity purification of the susceptibility and protective HLA ~ molecules was performed and the naturally processed peptides were eluted and sequenced by Edman degradation. The data obtained was used for motif prediction of HPY 16 E6, E7, Ll …
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