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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"immobilized enzyme"”.
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Investigation of extracorporeal immobilized enzyme device : a potential treatment for blood deheparinization
Thesis (Sc.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1999.
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Flow Injection Determination of Sugars by Combining Immobilized Enzyme Reactors and Chemiluminescence Detection
… detection with the inherent selectivity of enzyme reactions. The determination of glucose by enzymatic oxidation in the presence of glucose oxidase to produce gluconic acid and hydrogen peroxide and subsequent detection of the hydrogen peroxide produced by chemiluminescent reaction with …
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The Determination of Glucuronides in Biological Fluids by Lucigenin Chemiluminescence (beta-Glucuronidase, Glucuronic Acid, Immobilized Enzyme, (Hplc))
The use of certain chemiluminescence reactions as detection techniques for organic compounds has been explored during the past decade. In this research project the chemiluminescence reaction between alkaline lucigenin (10,10'-dimethyl-9,9'-biacridinium nitrate) and glucuronic acid (an important …
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Novel Potentiometric Enzyme Electrode Based on the Fluoride Ion-Selective Sensor.
… the determination of hydrogen peroxide using an immobilized enzyme and a fluoride ion-selective electrode. The reaction between hydrogen peroxide and certain organo-fluoro compounds is catalyzed by the enzyme horseradish peroxidase to release fluoride ions.
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Development of immobilization and drying methods of enzymes on support particles for enzymatic gas-phase reactions
… species alcohol dehydrogenase (ADH T) were immobilized by physical adsorption method. The effects of various immobilization and drying process parameters on the residual activity and the protein loading of the immobilized enzyme preparation were studied and thereby different optimum …
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Enzymatic fuel cells via synthetic pathway biotransformation
Enzyme-catalyzed biofuel cells would be a great alternative to current battery technology, as they are clean, safe, and capable of using diverse and abundant renewable biomass with high energy densities, at mild reaction conditions. However, currently, three largest technical challenges for …
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ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΜΙΑΣ Β-ΓΛΥΚΟΣΙΔΑΣΗΣ ΚΑΙ ΜΙΑΣ Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗΣ ΑΠΟ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ. ΕΦΑΡΜΟΓΗ ΤΩΝ ΕΝΖΥΜΩΝ ΣΤΗΝ ΥΔΡΟΛΥΣΗ ...
TWO ENZYMES A B-GLUCOSIDASE AND A B-GALACTOSIDASE WERE ISOLATED AND CHARACTERIZED FROM BARLEY SEEDS. THE B-GLUCOSIDASE IS A SINGLE BASIC POLYPEPTIDE (PI>8,5) WITH A MR OF 53.000 ACTING OPTIMALLY AT PH 4.5-5.0. THE B-GALACTOSIDASE IS COMPOSED OF TWO SUBUNITS WITH A MR OF 42.000 AND 33.000 …
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Polianilino ir pilipirolo, aukso nanodalelių bei įvairių elektronų pernašos tarpininkų taikymas gliukozės biologiniuose jutikliuose /
… the solution of glucose, oxygen and dissolved enzyme - catalyzed reaction that formed a strong oxidizing agent hydrogen peroxide and gluconic acid which allows the initiation of polymerization reaction process. Adapted polymer synthesis method demonstrated the possibility of polyaniline and …
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Polianilino ir polipirolo, aukso nanodalelių bei įvairių elektronų pernašos tarpininkų taikymas gliukozės biologiniuose jutikliuose /
… the solution of glucose, oxygen and dissolved enzyme - catalyzed reaction that formed a strong oxidizing agent hydrogen peroxide and gluconic acid which allows the initiation of polymerization reaction process. Adapted polymer synthesis method demonstrated the possibility of polyaniline and …
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Microfluidic Interfaces for Mass Spectrometry: Methods and Applications
… by implementing on-chip protein digestion on immobilized enzyme depots.
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Production of lactic acid by microbial fermentation of hemicellulose sugars
… Xylose is converted into xylulose by the immobilized xylose isomerase which helps to promote the utilization of xylose by the L. pentosus. To facilitate the recycle and reuse of the immobilized xylose isomerase, the immobilized enzyme (65 g) was packed in a fixed bed reactor with a …
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Modification of soybean proteins by immobilized proteases
Trypsin and alpha-chymotrypsin were immobilized on nylon pellets or porous glass by covalent methods to change molecular properties and functional characteristics of soybean proteins. The amount of trypsin immobilized on nylon pellets using the glutaraldehyde method was high when the pellets were …
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Inkless Soft Lithography: Utilizing Immobilized Enzymes and Small Molecules to Pattern Self-Assembled Monolayers Via Catalytic Microcontact Printing
… uses a chemical reaction between a stamp-immobilized catalyst and surface bearing cognate substrate to transfer pattern in the areas of conformal contact. By using pre-assembled cognate surfaces, the approach extends the range of surfaces readily amenable to patterning while obviating …
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Cloning, expression, charakterization and immobilization of carbonyl reductase from Candida parapsilosis
… cloning, expression and identification of this enzyme had failed so far, which might be due to failures in the quite old literature data. Recent characterization of the commercially available wild type CPCR (wtCPCR) showed that certain biochemical characters were in good agreement with the data …
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Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer
… peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing applications and their stability and activity were determined …
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A systematic study of enzyme-nanomaterial interactions for application in active surface decontamination
Enzymes are a catalytic class of proteins that possess high specificity, selectivity and biocompatibility, which makes them ideal for multiple applications in industrial production and biotechnology. However, the use of enzymes in such applications is limited due to their low operational stability …
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Übertragung der Produktion hydrophober Substanzen mit immobilisierten Biokatalysatoren in den technischen Labormaßstab
… organic solvent. As an example, in this work the enzyme carbonyl reductase from Candida parapsilosis (CPCR) was chosen to convert stereospecifically Acetophenone to (S)-1-Phenylethanol while oxidizing the cofactor NADH+H+. The spent cofactor was reduced again in an enzymatic regeneration process …
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Charakterisierung und Anwendung eines gelstabilisierten Zweiphasensystems für die asymmetrische Synthese neuartiger Benzoine mit immobilisierter Benzaldehydlyase
… solubility have a negative influence on the enzyme activity and the product quality. This thesis describes the development of a gel-stabilized two-phase system that enabled the application of the recombinantly produced benzaldehyde lyase derived from Pseudomonas fluorescens in organic …