Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"iTRAQ"”.
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The Role of Proteomics in Colorectal Cancer Biomarker Discovery
… verification using 2 proteomic platforms – iTRAQ and label free. I identified that the use of depletion methods along with iTRAQ and orbitrap analysis of the generated peptides yielded the best data. Using these technologies I was able to investigate the relative abundance of proteins …
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A proteomic investigation of the immune response of the South African abalone, Haliotis midae
… for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS was employed to investigate H. midae proteome changes in response to Vibrio anguillarum challenge. A preliminary iTRAQ challenge trial was conducted which identified a putative early (1 and 2 hours post-injection) and …
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Analisi proteomica quantitativa del fluido lacrimale per la diagnosi e la terapia farmacologica della Vernal Keratoconjunctivitis
… sono stati trattati tramite la tecnologia iTRAQ, seguita dall analisi dei campioni tramite 1D-LC-MALDI MS/MS. Durante la prima fase (biomarker discovery) sono stati realizzati cinque esperimenti iTRAQ 4-plex (due campioni di controllo e due patologici in ogni esperimento). I risultati …
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Investigation of protein induction in vascular-targeted strategies.
… MALDI-MSI, LC-ESI-MS/MS, LC-MALDI-MS/MS with iTRAQ labelling and immunohistochemistry intended to provide cross validation of the effects post administration of vascular disrupting agent CA-4-P. Two mouse fibrosarcoma models (expressing VEGF120/ VEGF188 isoforms only) following treatment with …
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high-throughput techniques
… A combination of microarray and 4-plex iTRAQ based approach was adopted to study the effects of rapa on MV4-11 and THP-1 cells and an 8-plex iTRAQ based methodology was employed to profile the proteome of the MV4-11 and HL-60 cells treated with GEN. We found that rapa had potent …
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A proteomic approach to investigate the response of tef (Eragrostis tef) to drought
… tissues at 80% RWC. Proteomic analyses using iTRAQ mass spectrometry coupled to peptide OFFGEL fractionation and appropriate database searching with the Tef Extended and Liliopsida databases enabled the generation of three dataset results. These datasets, each contained a substantial amount of …
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A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage
… pooled media collected, labeled with one of four iTRAQ labels and subjected to nano-2D-LC/MS/MS on a quadrupole time of flight instrument. Peptides were identified and quantified using Protein PilotTM, and MATLAB scripts used to obtain protein ratios. These results were analyzed using different …
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Proteomic profiling of matched normal and tumour tongue biopsies from smokers and non-smokers. Oncoproteomic applications for oral tongue squamous cell carcinoma biomarker discovery
… and causative agents, specific to smoking. An iTRAQ proteomic approach was used to profile protein changes in matched normal and tumour samples from male non-smoking (n=6) and smoking patients (n=6) with tongue carcinomas revealing identification of potential targets specific to cancer. Samples …
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Proteomic profiling of matched normal and tumour tongue biopsies from smokers and non-smokers. Oncoproteomic applications for oral tongue squamous cell carcinoma biomarker discovery
… and causative agents, specific to smoking. An iTRAQ proteomic approach was used to profile protein changes in matched normal and tumour samples from male non-smoking (n=6) and smoking patients (n=6) with tongue carcinomas revealing identification of potential targets specific to cancer. Samples …
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Proteomic profiling of matched normal and tumour tongue biopsies from smokers and non-smokers. Oncoproteomic applications for oral tongue squamous cell carcinoma biomarker discovery
… and causative agents, specific to smoking. An iTRAQ proteomic approach was used to profile protein changes in matched normal and tumour samples from male non-smoking (n=6) and smoking patients (n=6) with tongue carcinomas revealing identification of potential targets specific to cancer. Samples …
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ECLIPSED DUAL DISTRIBUTION OF MITOCHONDRIAL PROTEINS IN EUKARYOTES: UBIQUITINATION IN YEAST MITOCHONDRIA AS A PARADIGM
… the ubiquitination machinery. Additionally, the iTRAQ proteomics approach applied in the current thesis sets the ground for systematic screening of dual targeting regulation. We believe that the present studies will have an impact on discovery of new molecular events by which dual targeting, …
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Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress
… tags for relative and absolute quantitation (iTRAQ) technologies. Using the 2DE approach, a total of 438 proteins spots were reproducibly detected and analysed of which 18 protein spots were shown to be up-regulated in response to dehydration. These proteins contained both regulatory and …
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THE BIO-MOLECULAR ANALYSIS OF TITANIUM DIOXIDE NANOPARTICLES EXPOSURE ON CARDIOMYOCYTES
… tags for relative and absolute quantitation (iTRAQ™) was proposed to identify these biological endpoints. Using the H9c2 cardiomyocyte as an <i>in vitro</i> model, titanium dioxide (TiO₂) NPs was applied to understand how TiO₂ NPs can affect the heart. The study identified the localization of …
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PROTEOMICS INSIGHTS INTO MOLECULAR MECHANISMS OF TRIPTOLIDE AGAINST COLORECTAL CANCER
… Tags for Relative and Absolute Quantitation (iTRAQ) labeling followed by MS/MS analysis. The study of networks provides some insight into the molecular mechanisms of triptolide against colorectal cancer. In my second project, direct binding targets of triptolide were identified, by using …
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Proteomics Study of Pre-Harvest Sprouting in Wheat
… periods via a cutting-edge technology called iTRAQ. Proteomic analysis revealed that 190 differentially expressed proteins might be involved in various cellular functions, such as carbohydrate metabolism, nitrogen metabolism, stress response, redox regulation, ATP synthesis, and protein …
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Emerging concepts in the pathophysiology of acute pancreatitis
… advanced proteomic techniques of isobaric tags (iTRAQ™) for relative protein quantitation together with liquid chromatography–tandem mass spectrometry for the identification of the component proteins, the first comprehensive description of the normal (fasted and fed) rodent ML proteome is …
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The Influence of Preovulatory Estradiol on Uterine Transcriptomics and Proteomics Around Maternal Recognition of Pregnancy in Beef Cattle
… were analyzed using a 2D LC-MS/MS based 8plex iTRAQ method. RT-PCR data were analyzed using the MIXED procedure in SAS. Transcript abundances in the endometrium were quantified using kallisto, differentially expressed genes (DEGs) were determined using DESeq2 (FDR2), and IPA was used for …
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Oncoproteomic applications for detection of breast cancer. Proteomic profiling of breast cancer models and biopsies
… and phenotype specific signature. An 8-plex iTRAQ quantification strategy was employed to compare proteomic profiles of a range of breast cancer and ‘normal-like’ cell lines with primary breast epithelial cells. From this, 2467 proteins were identified on Orbitrap Fusion and Ultraflex II, of …
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Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids
… tags for relative and absolute quantitation (iTRAQ) technology to correlate MT1-MMP increase with proteomic profiles in MCF-7 breast cancer cell grown under hypoxic, serum-free and 3D MCTS conditions. More than 3500 proteins were identified, which were clustered into groups based on response …
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