Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 104 for “"iPSCs"”.
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Integrative genomic analysis of Neurogenin2 reprogramming of human iPSCs
… results in optimised transgene expression in hiPSCs (Opti-Ox), and consequently yields highly homogenous cultures of neurons within less than four days. The mechanisms that mediate this remarkable cellular metamorphosis however remain poorly understood. To explore this, I first sought to …
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MULTISCALE TECHNOLOGIES FOR ENGINEERING AND CRYOPRESERVING OVARIAN TISSUES AND HUMAN IPSCs
… Human induced pluripotent stem cells (hiPSCs) hold tremendous potential for tissue engineering and cell-based medicine, as they have the capacity of differentiating into ectodermal, mesodermal, endodermal, and germ cell lineages. In recent years, research into differentiating hiPSCs into …
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From iPSCs to Engineered Heart and Liver Models: Modulating Genetic and Extracellular State for Maturity
The heart and liver are central to human physiology yet remain major sites of drug-induced toxicity, driving the need for robust, scalable, and physiologically relevant in vitro models. Induced pluripotent stem cell (iPSC)–derived hepatocyte-like cells (HLCs) and cardiomyocytes (iPSC-CMs) offer …
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AR-A IPSCS: GENERATION OF AN ADVANCED CELLULAR MODEL TO INVESTIGATE A NOVEL THERAPEUTIC APPROACH FOR SBMA
… generation of induced Pluripotent Stem Cells (iPSCs) expressing AR-A (AR-A iPSCs). Starting from an SBMA patient-derived iPSC line which expresses AR with a polyQ tract of 51Qs (AR-Q51 iPSCs), CRISPR/Cas9 gene editing technique was exploited to block the translation of the ARpolyQ and allowing …
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Evaluation of Myogenic Differentiation Potential of BMD iPSCs in vitro and the Effect of Ubiquitination Inhibitors on Dystrophin Stability in vivo
… a promising strategy. The application of human iPSCs in tissue regeneration also serves as a potential therapeutic strategy. This study aims to test the efficacy of selected drugs interfering with the dystrophin polyubiquitination using BMD iPSCs as the model system. The hypothesis is that …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… the resulting induced pluripotent stem cells (iPSCs) have provided an alternative to conventional gene therapies thus far attempted for CF. Here we describe the <em>in vitro</em> generation of iPSCs starting from fibroblast cells obtained from a CF patient. These cells, like embryonic stem …
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Modeling Cancer Using Li-Fraumeni Syndrome Patient-Derived Induced Pluripotent Stem Cells
… fibroblasts to induced pluripotent stem cells (iPSCs), and further differentiate these iPSCs into mesenchymal stem cells (MSCs) then to osteoblasts (OBs), the cells from which osteosarcomas originate. Interestingly, LFS iPSC-derived osteoblasts recapitulated the osteosarcoma phenotype, creating …
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Investigation of genetic PIK3CA activation in genome-edited human pluripotent stem cells
… series of human induced pluripotent stem cells (iPSCs) with endogenously expressed, activating PIK3CA mutations. This involved the optimisation of a CRISPR/Cas9 protocol for efficient knockin of different PIK3CA variants into human iPSCs. An isogenic iPSC series was established with cells …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
<p>Induced pluripotent stem cells (iPSCs) are one of the most promising new technologies in the field of regenerative medicine. iPSCs can be generated directly from a patient’s own fibroblast cells then converted into any cell type, thus overcoming challenges associated with embryonic stem cells …
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Single-cell analyses of cellular reprogramming and embryonic stem cells
… state (termed induced pluripotent stem cells, iPSCs) by simply overexpressing four transcription factors: Oct4, Sox2, c-Myc, and Klf4. During cellular reprogramming, only a small fraction of cells become iPSCs. Previous analyses of gene expression during reprogramming were based on populations …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… porcine induced pluripotent stem cells (iPSCs). This reprogramming vector was verified first in murine cells, the system in which it was developed. Successful production of both murine and porcine iPSC lines was achieved. Both showed alkaline phosphatase activity, immunofluorescence for …
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An omics study into the molecular impact of autosomal dominant APP and MAPT mutations on the cerebral cortex
… on the differentiation of patient-derived iPSCs carrying MAPT E10+16 and APP duplication mutations and isogenic control iPSCs into neuronal cultures using the directed differentiation protocol. I first describe the CRISPR/Cas9 strategy to correct APP copy number in iPSCs derived from …
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MICROGLIA-SUFFICIENT BRAIN ORGANOID
… from induced human pluripotent stem cells (iPSCs) has been shown to recapitulate the human embryonic brain development process. However, such organoids lack microglia. Here, we generated microglia-sufficient brain organoids by co-culturing brain organoids and primitive-like macrophages …
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CORRECTION OF THE GENETIC DEFECT IN INDUCED PLURIPOTENT STEM CELL LINES THROUGH CHROMOSOME TRANSPLANTATION
… generation of induced pluripotent stem cells (iPSCs) by Yamanaka and Takahashi in 2006, through the use of four reprogramming factors (Oct4, Sox2, Klf4 and c-Myc). This finding enabled the generation of iPSCs from adult patient-specific cells, overcoming numerous ethical concerns by the use of …
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… lab uses human induced pluripotent stem cells (iPSCs), as an attractive alternative supply, as iPSCs can be cultured indefinitely and differentiate into almost any cell type. Using a technique called forward programming, we over express three key haematological transcription factors (TFs), …
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Development of collagen-based scaffolds for differentiation of induced pluripotent stem cells
… for direct seeding and lineage progression of iPSCs. In this work, three formulas of collagen-based scaffolds were developed by fabricating poly(ethylene glycol) diacrylate (PEGDA) into the collagen hydrogel to form an interpenetrating network (IPN). Stability test showed significant …
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Investigating the effects of tau mutations in induced pluripotent stem cell-derived neurons
… I differentiated induced pluripotent stem cells (iPSCs) derived from control subjects and people with the N279K or V337M MAPT mutations to monolayer neurons. There was an increase in the proportion of neurons containing tau phosphorylated at the AT8 epitope in N279K cultures after 50 days, and …
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Effect of Parkinson’s disease-related alpha-synuclein abnormalities on the maturation of distinct iPSC-derived neuronal populations
… them into induced pluripotent stem cells (iPSCs), and differentiating iPSCs into neurons. For this project, iPSCs derived from two PD patients, one harbouring the A53T SNCA mutation, the other a SNCA triplication, and three healthy individuals, were employed. In the initial stage, I …
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