Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"iPS cell"”.
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Modelling Brugada Syndrome using induced pluripotent stem cells
… dermal fibroblasts to induced pluripotent stem (iPS) cells and to differentiate these into cardiomyocytes with the same genetic background provides a novel approach to studying inherited cardiac channelopathies with advantages over existing model systems. Whilst this technique has enormous …
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Modeling Alzheimer's Disease Using Cellular Reprogramming Technologies
Two cellular reprogramming technologies have emerged that demonstrate that cell-fate can be converted by ectopic expression of defined transcription factors: induced pluripotent stem (iPS) cell technology and induced neuronal (iN) cell technology. These recent advances in cell reprogramming …
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Defining and Controlling the Subtype Identity of Human Stem Cell-Derived Motor Neurons
One cardinal promise of stem cell research is that many intractable, common, and poorly understood diseases may be studied in an entirely new way: in vitro in the specific human cell types affected in vivo. Embryonic stem (ES) cells have the pluripotency to generate all somatic cells types, and the …
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Induced pluripotency and reprogramming by defined factors
… genome is the foundation for epigenetics and cellular differentiation. Somatic cell nuclear transfer experiments (SCNT) provided unequivocal proof of nuclear equivalency between adult somatic cells. However the molecular mechanisms of somatic cell reprogramming have remained largely a mystery. …
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An improved method for generating oligodendrocyte progenitor cells from murine induced pluripotent stem cells
Cell based therapies aiming to restore myelin in the central nervous system offer great hope for treatment of numerous conditions, ranging from multiple sclerosis and the leukodystrophies, to traumatic CNS injury. The oligodendrocyte progenitor cell (OPC) gives rise to functional oligodendrocytes …
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Modularity in Somatic Cell Reprogramming
Reprogramming relies on the concept that cellular fate is plastic, and therefore a considerable effort has been made to develop molecular strategies that reset the epigenome to achieve distinct cell fates, including stem cells. Deriving induced pluripotent stem (iPS) cells has thus emerged as a …
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Investigation of pancreatic beta cell differentiation and function, and global profiling analysis of diabetes serum for the identification of disease biomarkers
… compared to glucose responsive MIN6 cells. Functional investigations involving knockdown of mir-200a, mir-130a and mir-410 in MIN6 cells exhibiting GSIS resulted in reduced GSIS. This suggested that these three miRNAs functioned in supporting the capability of MIN6 cells to secrete …
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Establishment of the epigenetic landscape in mammalian embryonic stem cells
… phenomena has the potential to enrich both stem cell biology and therapeutics, as well as offer insight into various pathologies. While the enzymatic machinery responsible for these transitions is well known, it is their localization to specific genomic regions that controls cell fate, and this …
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Generation of induced progenitor-like (iPL) cells using interrupted reprogramming
… medicine is constrained by suboptimal cell sources with either limited or uncontrolled proliferation and/or incompletely restricted differentiation. We developed a novel "interrupted reprogramming" strategy without traversing the pluripotent state, to generate "induced Progenitor-Like …
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Genetic and Epigenetic Interactions in in vivo and in vitro Reprogramming
… pathogenesis and induced pluripotent stem: iPS) cell production, an essential step for reprogramming is acquisition of self-renewal. In hematopoietic cells, HOX genes are partially responsible for self-renewal, and HOX gene dysregulation commonly occurs in acute myeloid leukemia: AML). HOX …
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Characterization and application of human pluripotent stem cell-derived neurons to evaluate the risk of developmental neurotoxicity with antiepileptic drugs in vitro
… animal experiments. Pluripotent stem cell-based systems can facilitate developmental neurotoxicity studies because disturbances in nervous system development can be modeled in vitro. In this study, neurons derived from embryonal carcinoma (EC) and induced pluripotent stem (iPS) cells, …
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Instructing naïve pluripotency ; JAK/STAT3 and Nanog in reprogramming
Reprogramming of somatic cells to pluripotency is a topic of extensive research driven by the potential of induced pluripotent cells in in vitro disease modelling, drug screening and regenerative medicine. The pluripotency associated transcription factor Nanog has been subject to a lot of …
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Treatment-resistant ophthalmoplegia in Myasthenia gravis: extraocular muscle pathology, the role of TGFβ1 and the derivation of induced pluripotency towards 'disease-in-a-dish' modeling
… microenvironment with a large satellite cell niche, a distinct muscle fibroblast population, different transcriptional and cellular signaling pathways and fewer intrinsic complement regulatory proteins to protect them against antibody- activated complement-mediated damage. We hypothesised …
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THE CELL CYCLE GENES REGULATION OF IPS CELLS AND THE ROLE OF SWI/SNF CHROMATIN REMODELING ENZYMES DURING THEM DIFFERENTIATION
Lo stato di cellula pluripotente può essere raggiunto con l'espressione ectopica di alcuni fattori di trascrizione che possono riprogrammare le cellule somatiche. (Takahashi, K., e Yamanaka, S., 2006; Takahashi, K., e Yamanaka, S., 2007).
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Development of single-molecules techniques to study native α-Synuclein aggregates
… αSyn. The αSyn content of aggregates secreted by cells was investigated using an iPS cell line derived from a Parkinson’s diseased patient harbouring a triplication of the SNCA gene, which encodes for αSyn, on one allele and a derivative of this cell line with the gene deleted, SNCA knock-out. The …