Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"iCLIP"”.
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Probing RNA binding specificities of AID/APOBEC proteins by iCLIP
… I applied cross-linking and immunoprecipitation (iCLIP), an unbiased technique that allows identification of protein-bound RNAs with nucleotide resolution in living cells. As a first approach, I adapted the technique for its use in yeast and probed the RNA binding of AID and APOBEC3G, revealing …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… at nucleotide resolution. In vitro spliceosome iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation) was initially developed targeting the helicase Prp16 bound to spliceosomal complex C. The obtained binding profile shows that Prp16 contacts the intron, about 15 …
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Etablierung eines quantitativen transienten Expressionssystems zur funktionellen Charakterisierung von RNA-Bindeproteinen in der posttranskriptionellen Genregulation
… nucleotide crosslinking and immunoprecipition (iCLIP) entwickelt. Diese Technik erlaubt es, die Zieltranskripte von RBPs zu identifizieren und auch die für eine Bindung verantwortlichen RNA-Sequenzen zu bestimmen. Das glycinreiche RBP AtGRP7 war das erste RBP in Arabidopsis thaliana (A. …
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Nuclear binding and translation regulation by FMRP through novel associated protein and putative RNA helicase MOV10
… bind G quadruplex structures specifically. Using iClip, we identified MOV10 specific mRNA targets as well as identifying binding sites of MOV10. We also found that Ago2 binding sites influenced the fate of MOV10 clip targets, suggesting a role of MOV10 acting as a facilitator or inhibitor of miRNA …
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Human La Function in Expression of Coding Transcripts
… of La with actively translating messages. Using iCLIP, we identify novel hLa associated targets. Taken together, these results suggest that hLa is trafficked into the cytoplasm in response to cellular stress in order to associate with IRES containing messages, and this interaction may occur …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
… splicing networks. Using high-resolution iCLIP and RNAseq, we identified many Rbfox2-mediated proteincoding splicing events and nearly 300 additional events, in particular those within RBPs, that are coupled to nonsense-mediated mRNA decay (NMD). Regulation of NMD-coupled splicing by …
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The Regulation of Microrna Biogenesis By Ribosome-Interacting Proteins
… associate with miR-200 precursors through iCLIP analysis.</p> <p>In summary, the ribosome-associated proteins nucleolin and RRBP1 were identified and characterized as two novel proteins involved in miRNA biogenesis, each forming feedforward miRNA loops that regulate distinct cellular …
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Role of DDX RNA helicases in cancer cells
… in breast cancer (MCF7) cells by performing iCLIP experiments. This generated two very distinct RNA binding profiles: DDX3X preferentially bound exonic regions of mRNAs encoding translational factors, whilst DDX54 preferentially bound non-coding RNAs and intronic regions of mRNAs encoding …
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Elucidating the functions of RNA helicases DDX3X and DDX3Y in normal and malignant B cells
… RNA and protein interactome studies using iCLIP and mass spectrometry. This revealed subtle differences in the pattern of RNA binding and interaction with the translation initiation machinery. Specifically, DDX3X showed preferential interaction with eIF3 subunits, suggesting possible …
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Molecular requirements for FMRP and RNA helicase MOV10 in the translational regulation of co-bound mRNAs
… nucleotide Cross-linking Immunoprecipitation (iCLIP) as well as identified FMRP-MOV10 co-bound targets by comparison of this data with published FMRP CLIP data. We then showed that FMRP recruits MOV10 to a subset of mRNAs, presumably to resolve RNA secondary structure and facilitate AGO …
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RNA helicase MOV10 is essential for gastrulation and normal CNS development
… in vitro assay. In addition, both the RNA-IP and iCLIP analysis of Mov10 RNA targets at P0-P1 brains followed by functional annotation showed that Mov10 bound significantly more mRNAs involved in cytoskeletal and actin binding. To investigate the role of Mov10 in cytoskeletal dynamics, we created …
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RNA binding proteins ZFP36 and ZFP36L1 limit CD8+ T cell differentiation and effector function
Post-transcriptional regulation of gene expression is mediated in part by RNA binding proteins (RBPs). The ZFP36 family of RBPs are key regulators of gene expression in the immune system. The founding member ZFP36 is a well-known regulator of cytokine mRNA stability. Paralogs ZFP36L1 and ZFP36L2 …