Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"hypoxanthine"”.
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Purification and characterisation of plasmodium falciparum Hypoxanthine phosphoribosyltransferase
Malaria remains the most important parasitic disease worldwide. It is estimated that over 500 million infections and more that 2.7 million deaths arising from malaria occur each year. Most (90%) of the infections occur in Africa with the most affected groups being children of less than five years …
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The biochemical and molecular basis of Hypoxanthine-guanine phosphoribosyltransferase deficiency
Hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyses the first step in purine salvage. A complete deficiency of the enzyme results in the devastating neurological symptoms of the Lesch-Nyhan syndrome. The Lesch-Nyhan syndrome is characterised by purine overproduction leading to, …
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The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
… forms of P. falciparum, and Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HPRT) in E. coli using the pET expression system. The cDNA encoding the ORF of HPRT was amplified by PCR and transformed into E. coli cells using standard methods. Expression was induced by IPTG and …
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Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)
The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of …
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Purine transport in plasmodium falciparum
… of adenosine uptake was seen to be faster than hypoxanthine in parasite-infected cells. It was observed that transport of hypoxanthine and adenine through the hFNT1 was unexpectedly inhibited by furosemide. This inhibition by furosemide of [3H]-hypoxanthine and [3H]-adenine uptake was not …
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Kinetic and metabolic studies in HPRT deficiency
… (T.K.), was first diagnosed as having a partial hypoxanthine-guanine phosphoribosyltransferase deficiency in 1978 when he was 20 years old. At presentation, he complained of a colicky loin-pain which radiated into his groin, and that he had had dark urine for a month. He was shown to have …
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The Enzymatic Oxidation of Aldehydes by Xanthine Oxidase
… which catalyzed the oxidation of xanthine and hypoxanthine was recognized. The name given the enzyme was derived from its earliest recognized action, hence xanthine oxidase. Although little is known about the biological functions of the enzyme, the high affinity shown for xanthine and …
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INVESTIGATION OF INOSINE AND HYPOXANTHINE AS BIOMARKERS OF CARDIAC ISCHEMIA IN PLASMA OF NON-TRAUMATIC CHEST PAIN PATIENTS AND A RAPID ANALYTICAL SYSTEM FOR ASSESSMENT
… method was developed and utilized for inosine, hypoxanthine and other adenosine triphosphate (ATP) catabolic by-products in Krebs-Henseleit (Krebs) buffer solution, with analysis on perfusate samples from isolated mouse hearts undergoing 20 min acute global ischemia. The HPLC-UV method was …
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Aspects of purine and pyrimidine metabolism
… phosphate (Pi) on the uptake and metabolism of hypoxanthine by erythrocytes has been studied in Chapter 2. Uptake of hypoxanthine and accumulation of inosine 5'-monophosphate (IMP) were markedly increased at acid pH, high external phosphate concentrations, and low pO₂. Release of accumulated IMP …
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Effect of Procedural Pain on Purines, MDA and Allantoin in Premature Infants
… between procedural pain, ATP utilization (hypoxanthine and uric acid), and oxidative stress (MDA and allantoin) in premature infants. Through these studies we found that 1) a single heel-lance significantly increases plasma uric acid in rabbit kits, 2) tape removal increases MDA and …
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Base modified peptidic nucleic acids: synthesis and crystallographic analysis of intermediates
… (D), isoGuanine (isoG), xanthine (X) and hypoxanthine (H) were identified for incorporation into PNAs targeted to DNA, with the promise of increased hybrid stability over extended pH ranges together with improvements over the use of adenine (A) in duplex formation, and cytosine (C) in …
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Purine Degradation and Ureide Catabolism by Soybean (Glycine Max (L.) Merrill); Cell Suspension Cultures
… caused accumulation of uric acid and hypoxanthine, respectively, in cell suspensions fed radiolabelled IMP and effectively blocked growth on IMP, but not allantoin, as a sole nitrogen source.
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The effect of sprint training on human skeletal muscle purine nucleotide metabolism
… degradation prcducts in the plasma (ammonia, hypoxanthine) during exercise, and in recovery from a 30 s "all out" sprint was also examined. Finally, this thesis studied the influence of sprint training on performance variables pertinent to sprint exercise.
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Methanocaldococcus jannaschii and the Recycling of S-adenosyl-L-methionine
… for the biosynthesis of methylglyoxal. Lastly, hypoxanthine produced from phosphorolysis of inosine, MTI, and 5'-dI was demonstrated to be reincorporated through the hypoxanthine/guanine phosphoribosyltransferase (Hpt) to IMP. Together these reactions represent novel pathways for the salvage of …
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Defined Dimensional Alterations in Enzyme Substrates. Synthesis and Enzymatic Evaluation of Some Lin-Naphthopurines
… laterally extended dimensional derivatives of hypoxanthine and xanthine. These compounds, lin-naphthohypoxanthine and lin-naphthoxanthine, exhibited intense fluorescence. lin-Naphthoxanthine was not oxidized to lin-naphthouric acid by xanthine oxidase but functioned as a noncompetitive …
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A clamp ligation method for point mutational spectrometry : marked increase in scanning range for the human genome
… with a natural clamp, a part of the human hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene (cDNA-bp 223-318), was analyzed using both the natural and ligated clamps. A sensitivity of 2 x 10-5 in human cells was demonstrated using the ligated clamp as opposed to 5 x 10-6 using the …
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Nucleobase deamination as a biomarker of inflammatory processes
… lead to incorporation of the purine precursors hypoxanthine (I) and xanthine (X) into DNA. This can be regarded as another endogenous process causing DNA damage that may lead to human diseases such as cancer ...
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Enzyme Analysis of Oxidatively Modified Proteins Post-TBI
… cytochrome C oxidase, ATP synthase, and hypoxanthine-guanine phosphoribosyltransferase, that have been identified as excessively nitrated following the administration of GCEE post-TBI. Adult male Wistar rats were divided equally into three groups: sham, saline, and GCEE. Rats in all …
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Modified Purines. Rearrangement Through 5-Substituted Purine Intermediates
… are analogues of N(9)-substituted guanines, hypoxanthines, and xanthines. Structures were established in this series (1) on the basis of precursors and routes of synthesis, infrared spectra, mass spectra, ('1)H and ('13)C nuclear magnetic resonance spectra, and X-ray crystallographic analysis …
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Abrogation of serine metabolism exacerbates intestinal epithelial inflammation via C/EBPβ-NF-κB pathway
… and ELISA. Metabolic supplements formate and hypoxanthine were tested to mitigate the overactivation of inflammation. Total starvation was assessed by using serine deprivation medium and the PHGDH inhibitor BI-4916. RNA sequencing and proteomic analyses were performed to investigate molecular …
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