Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"hypomethylation"”.
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Diet, genetic and epigenetic signatures in women of childbearing age from a Mediterranean population: perspectives for public health
… association between folate deficiency and global hypomethylation, was found: women with folate deficiency had a 4 fold increased risk to be hypometylated compared with women with no folate deficiency (OR = 4; 95% CI: 1.3 11.8). In multivariate analysis, the association decreased with a borderline …
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GENOMIC AND EPIGENETIC APPROACHES IN THE CLINICAL AND PROGNOSTIC STRATIFICATION OF CHRONIC LYMPHOCYTIC LEUKEMIA
… approach was used to investigate global DNA hypomethylation affecting repeated sequences, such as long interspersed nuclear elements-1 (LINE-1), Alu and satellite α DNA (SAT-α DNA), reported to be associated with chromosomal instability. Our analysis was performed in a panel of 77 CLLs and 7 …
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Interrogating Dux4 Mrna 3′ End Processing
… cells due to two conditions. The first is hypomethylation of the subtelomeric D4Z4 repeats on chromosome 4. Each D4Z4 repeat on chromosome 4 is 3.3kb in length and contains the open reading frame for Dux4. Hypomethylation of the D4Z4 repeats primarily occurs due to contraction of the …
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Oct4 and Sox2 regulate methylation at the Igf2/H19 imprinting control region
… that both octamers were required for transgene hypomethylation in F9 cells and that Oct4 was present at the endogenous ICR. In addition, each of the octamers is flanked by a sequence that allowed cooperative binding of Oct4 and Sox2 in vitro. To determine at which developmental stage the …
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The Effect of the Microbiome on DNA Methylation
… unmethylated CpG islands, and global DNA hypomethylation. Microbiota-free mice are known to live longer than their normal counterparts, and microbial dysbiosis is known to be a hallmark of colorectal cancer. In order to determine the microbiota’s ability to impact DNA methylation patterns, …
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Analysis of methylation status of CGB genes in common nontrophoblastic cancer
… this study confirmed previously reported hypomethylation in trophoblastic cell lines. Hypomethylation of the genes was found in HeLa cell line which matches literature reported transcription studies. However, other results were discordant or inconclusive leading to no significant …
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The Regulation of Dna Methylation In Mammalian Development and Cancer
… For example, cancer cells usually show global hypomethylation and regional hypermenthylation, which have been implicated in genomic instability and tumor suppressor silencing, respectively. Although great progress has been made in elucidating the biological functions of DNA methylation over the …
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Investigating the molecular dynamics of gene re-silencing following treatment with epigenetic therapies
… was dependent on DNMT1 depletion and hypomethylation, nucleosome eviction and the acquisition of active histone marks at the MLH1 promoter. However, close inspection of the early stages of gene re-silencing showed that loss of expression occurred despite persistent hypomethylation. …
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Using Advanced In Vitro Models to Investigate the Adverse Effects of Methotrexate on the Developing Epigenome
… global average of CpG methylation, as well as hypomethylation and hypermethylation of CpGs genome-wide and at imprinted DMRs. Similarly, treatment of primordial germ cell-like cells, derived from the in vitro gametogenesis protocol as a proxy for the developing germline, also exhibited …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells (PGCs), the early embryo and in naïve embryonic stem cells (ESCs). Genome integrity is crucial during early development, as the germline DNA needs to be …
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Investigating HK2 as a potential therapeutic target in glioblastoma
… and enzymatic pathways of glucose metabolism. Hypomethylation was demonstrated in all biopsies (n=100) and cultures (n=15) compared to normal brain tissue; with average methylation of 4.6% compared to 26% respectively (p<0.0001) determined across 15-CpGs. A significant increase (p<0.0001) in …
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Investigation of DNA methylation at the promoter region of the aralkylamine N-acetyltransferase (AANAT ) gene in South African children with Autism Spectrum Disorder
… with ADOS-2 Modules 2 - and 3. In particular, hypomethylation was more common in participants assessed with Module 1 which is the module used to assess participants with little or no speech abilities. The transcription factor (TF) binding motifs for ZID (zinc finger protein with interaction …
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The roles of DNA(cytosine-5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
… a clinical study it was discovered that genomic hypomethylation of the LCR and promoter region correlated with carcinogenic progression. The HPV-16 genome could be demethylated using 5AzadC and MMS which led to the over-expression of the late genes, hence suppressing the early genes. DNMT1 and …
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Epigenetics, a signal sensor of dietary components, is involved in the hepatic gene regulation
… DNA immunoprecipitation (MeDIP) showed that DNA hypomethylation occurred in an upstream region of the distal promoter and two coding regions of the COX-2 gene in all three high fat groups. Site-specific hypomethylation of CpG at 5’ UTR (untranslated region) of COX-2 was confirmed with bisulfite …
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Alterations in Genomic 5-Hydroxymethylcytosine Level in Hepatocellular Cancer
… of certain tumor suppressor genes and hypomethylation of oncogenes have been shown to initiate HCC. 5-hydroxymethylcytosine (5-hmC), recently identified sixth base of the genome, can cause demethylation by its conversion to cytosine through reactions catalyzed by the TET enzymes. …
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Epigenetic Modification as a Therapeutic Target in BRAFV600E-mutated Metastatic Colorectal Cancer
… (DNMT) inhibitor treatment induced profound DNA hypomethylation in vivo, but minimal change in gene expression due to adaptive elevation of the repressive histone methylation, H3K27me3, leading to compensatory suppression of key tumor suppressor genes, including Wnt pathway genes. EZH2 inhibitors …
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Accumulation of perfluorooctanoic acid in intestine and lung tissues and its effect on gene expression across multiple pathways
… regulatory region of Tmprss2 exhibit significant hypomethylation patterns. Collectively, these findings suggest that PFOA is potentially contributing to the tissue specific toxicological effects.
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p53 acts to prevent the formation of pathological R-loops during epigenetic stress
… in cancers. Using Aza and Dac to induce DNA hypomethylation, data from DRIPc-Seq, Immunostaining, RNA-Seq and Mass spectrometry was combined with epigenetic analysis to uncover unique cellular responses following Aza and Dac treatment, with a particular focus on how treatment with these drugs …
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Sex-dependent and Sex-independent Gene Regulation after Peripheral Nerve Injury
… global hypermethylation in male PAG and global hypomethylation in female PAG after PNI. Moreover, I discovered genes that were differentially methylated in blood after PNI. A major discovery I made was that the methylation levels of the P2rx4 promoter differed by sex; After PNI, the P2rx4 …
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Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
… epigenetic changes, specifically H3K9me3 hypomethylation, that correlated with increased donor cell reprogrammability.
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