Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"hypervariable region"”.
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Short interval change in hepatitis C hypervariable region 1 in chronic infection. Are there treatment windows in the envelope?
… 50-80% of those acutely infected. HCV has three hypervariable regions (sections of the virus genome that appear to tolerate higher substitution rates) and one of these, Hypervariable region 1 (HVR1) has been recognised as a major target of the adaptive immune response. HVR1 quasispecies …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… aspects of HEV. Recently, we identified a hypervariable region (HVR) in the open reading frame 1 (ORF1) of HEV. The objectives of this dissertation were to utilize chicken and swine model systems to study the role of HVR in HEV infection in vivo, to determine the effects of HVR on …
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Epitope mapping of the E2 glycoprotein, including the hypervariable region 1, of the hepatitis C virus genotype 3a, in the context of humoral immune pressure
The hepatitis C virus (HCV) is an enveloped +ssRNA virus, belonging to the family Flaviviridae. HCV is notable for displaying extraordinary genetic diversity and variability, having seven recognised genotypes and over sixty subtypes. HCV is responsible for the disease known as hepatitis C, which is …
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A demographic and dietary history of ancient dogs in the Americas using ancient DNA
… of humans. Mitochondrial DNA sequences of the hypervariable region of ancient dogs were compared to modern and ancient American dogs to model dog demography and compare populations to identify shared haplotypes. This study identified multiple founding haplotypes, and suggested that dogs arrived …
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Analysis of mitochondrial DNA variation in the Egyptian population and its implications for forensic DNA analysis
… for rapid PCR amplification of the mitochondrial hypervariable regions was developed. A database of 261 mitochondrial hypervariable region I (HVI) sequences and 78 hypervariable region II (HVII) sequences was established from 261 adult Egyptians. A total of 113 polymorphic sites were reported in …
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Comparative Mitochondrial DNA Sequence Diversity in Isolated and Open Populations of Southern Flying Squirrels
… population were investigated. A 367 nucleotide hypervariable region of mitochondrial DNA was sequenced in individuals from each population. Individuals and populations were compared to assess relatedness. Higher sequence diversity was detected in the open and island populations. One island …
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T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand
… within a loop that is analogous to the fourth hypervariable region (HV4) of the $\beta$ chain, disrupted binding to QL9/L$\sp{\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\alpha$ chain variable region, these five residues are likely to be involved in …
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Das T-Zell-Rezeptor-Repertoire bei der Polymyositis
… on te natural length variation of the third hypervariable region (CDR3) of the rearranged T cell receptor gene: whereas a polyclonal T cell population shows a random, Gauss-distributed length variation of the CDR3, a clonally expanded population has a uniform CDR3 length, which can be …
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Investigating the C-terminal region of RalA: regulation through membrane dynamics and protein-protein interactions
… ~20 residues. However, this C-terminal hypervariable region is not only the main source of variation between RalA and RalB, but also serves as a membrane anchor via a polybasic region and prenylation and has been suggested to play key roles in modulation of the function of these …
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Characterization of a T Cell Receptor: Peptide/MHC Binding Interaction
… mutations in the complementarity determining regions (CDR) and fourth hypervariable region of the $\beta$ chain. Using these mutant scTCRs, we have identified V$\beta$ residues which are energetically important in the interaction of the 2C TCR with the anti-V$\beta$8 mAb F23.2, the …
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Candidate Bivalent HCV Vaccine: A Novel Approach To Vaccine Design for Hyper-variable Viruses
… explicitly designed to elicit AB to HVR1 (hypervariable region 1), the most variable epitope of any known human pathogen. Remarkably, the polyclonal response induced by vaccination was pan-genotypically neutralizing. These results support our hypothesis that physiochemically constrained …
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Characterising variation in the gene for the Dichelobacter nodosus Type IV fimbrial protein: Development of a molecular typing system
… development of a PCR specific for the variable region of the fimA gene. Cloning and sequencing of a single field derived amplimer and eight cultures currently being used in commercial footrot vaccines (Mallinckrodt Veterinary Ltd), is described. The rimA sequences exhibited varying degrees …
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Human Parainfluenza Virus 3: Genetic Diversity, Virulence and Antiviral Susceptibility
… of HPIV3 was developed and validated. A short hypervariable region in the HPIV3 genome was identified and evaluated as a potential candidate for subsequent phylogenetic analysis compared to whole genome data. This method was then applied to tracking an HPIV3 outbreak that took place on a …
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A seasonal comparison of the gut microbiome of the Southern Lesser Galago Galago moholi (A.Smith 1836)
… was used to sequence the 16S rRNA gene regions in order to quantify the diversity and abundance of the bacterial taxa. The hypervariable regions of the 16S rRNA gene were assessed in terms of the abundance and diversity of bacteria present. The four major phyla present were …
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Supplementing the studbook. Using genetic analysis to complement a captive breeding programme of an endangered primate, Hylobates moloch.
… of re-introductions analyses at three genetic regions were carried out, with DNA extracted from non-invasively collated faecal samples. In order to assay if captively bred individuals were representative of their wild conspecifics, the population was split into two groups representative of wild …
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Development and Characterization of Novel Ras Inhibitors
… of p120RasGAP to the plasma membrane with the hypervariable region (HVR) of H-Ras (GAP-CTH) or K-Ras (GAP-CTK) and to the Golgi using the HVR of H-Ras with insertion of a point mutation (GAP-CTH181S). We performed GST-RBD pull-downs on cells expressing each GAP construct and stimulated with …
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Streptococcal M protein and human C4BP
… antigenic variation due to an N-terminal hypervariable region (HVR). The HVRs of many M proteins bind the human complement regulator C4b-binding protein (C4BP), which down-regulates deposition of complement on the bacterial surface and thereby protects the bacteria against …
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Molecular characterization of Flavobacterium spp. isolated from Rainbow trout (Oncorhynchus mykiss) farmed in Southern Africa and development of a PCR-based tool for differentiating between isolates
… was achieved following PCR amplification of a hypervariable region of the 16S rRNA gene followed by high-resolution melt (HRM) analysis. Nine Flavobacterium isolates, presumed to be different species based on the phylogenetic analysis were identified and successfully differentiated using high …
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Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis
… was also developed for use in resource- limited regions. The study was conducted over two years (2014-2016) and examined 284 residual diagnostic samples. The cohort was drawn from patients attending a central London western travel medicine (WTM) clinic. The NAATs chosen for this study were a …
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Genetic diversity and hybridisation estimates of Arctocephalus tropicalis and A. gazella from Marion Island
In this study, hypervariable region I (HVRI) of the mitochondrial DNA (mtDNA) control region, and five microsatellite loci were used to assess genetic variability and the extent of hybridization between the two fur seals (Arctocephalus tropicalis and A. gazella), that occur on Sub-Antarctic Marion …
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