Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"hygromycin"”.
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Antibiotics that Inhibit 30S or 50S Ribosomal Subunit Formation: Hygromycin B, Quinupristin-Dalfopristin and XRP 2868.
… (Champney and Tober 2003). The aminoglycoside hygromycin B was examined in <em>Escherichia coli</em> cells for inhibitory effects on translation and ribosomal subunit assembly. The streptogramin antibiotics quinupristin-dalfopristin and XRP 2868 (NXL 103) were examined for similar effects on …
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MECHANICAL ABRASION AND ELECTROPORATION IN THE TRANSFORMATION OF INTACT PLEUROTUS OSTREATUS HYPHÆ and NUTRIENT-DEPENDANT RESISTANCE TO HYGROMYCIN B
… and Cordyceps millitaris to the antibiotic hygromycin B was tested on two common and one in-house solid growth media. The three species were grown on Potato dextrose agar, malt extract agar and an agar containing maltose, yeast-extract, peptone and glucose with concentrations of hygromycin B …
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Tissue culture and transformation of introducing genes useful for pest management in rice
… Taipei 309 using the hpt gene for resistance to hygromycin B as a selective marker. Transformed calli and regenerated plants were screened with hygromycin B, and the plants were then further tested for resistance to Liberty herbicide and Rhizoctonia solani. Methods were developed to screen …
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… tomato variety, M82. The construct contained hygromycin resistance (hyg), green fluorescent protein (GFP), and maize transposase (TPase) on the stable Ac element, along with a 35S enhancer tetramer and glufosinate herbicide resistance (BAR) on the mobile Ds element. An in vitro propagation …
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Modification of Vegetable Soybean Sugar Content via Gene Transformation
… selection in FG medium containing 25 $\mu$g/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or $\alpha$-galactosidase genes but Southern and northern …
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Studies on rice transformation and the use of transformed plants [electronic resource]
… the sensitivity of transgenic rice plants to hygromycin B and Liberty were developed, tested and used in this research. Four homozygous transformed plants were selected to make reciprocal crosses with their non-transformed parent cultivars Taipei 309 and Nipponbare. Their resistances to …
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Transformation of a Transposon Construct into Tomato for Functional Genomics Studies
… gene conferring resistance to herbicide Basta, hygromycin (HYG) gene conferring resistance to the antibiotic hygromycin and the green fluorescent protein (GFP) gene, which are driven by specific plant promoters. The selectable marker genes such as HYG and BAR were used to select the rare …
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Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… (uidA) gene and the selectable marker hygromycin phosphotransferase (hph) gene under the control of CaMV 35S promoter plus the maize Adh1 intron 6. Putative transformants were selected by culturing cells on medium containing hygromycin. Transgenic plants and calli were obtained …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… cell line (hTCEpi) transduced with the hygromycin-thymidine kinase gene (HyTK) as a viable cell source for the reconstruction of the corneal surface. METHODS: HyTK cells were cultured in KGM-2 serum-free culture media under hygromycin B selection. Limbal stem cell deficiency (LSCD) was …
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A Cloning-Free Recyclable System for CRISPR-Cas9 Mediated Mutant and Reversion Construction in Candida albicans Clinical Isolates
… plasmid containing a C. albicans-optimized Hygromycin B resistance gene (CaHygB) in place of the SAT1 nourseothricin resistance gene to serve as repair templates. PCR-amplification of these cassettes with primers containing sequences homologous to the flanking regions of the target gene …
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Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… contained GFP (green fluorescent protein) and hygromycin antibiotic-resistant selectable markers, and the antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence of transgenes …
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Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
… were confirmed by growing on the selection agent hygromycin, GUS assay, PCR amplification, southern blotting hybridization, for the presence of hygromycin and endoglucanase genes. However, based on RT-PCR analysis, only two transgenic lines were confirmed to produce mRNAs of the endoglucanase …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… a gene conferring resistance to the antibiotic hygromycin. The genes are placed under the control of the 35S CaMV (cauliflower mosaic virus) promoter and a NOS terminator. A plant catalase intron is inserted into the GUS reporter gene. The presence of GUS enzymatic activity, as a result of …
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Transformation der Plastiden und Mitochondrien bei höheren Pflanzen - Selektive Marker und Einsatzmöglichkeiten
… den Antibiotika Blasticidin, Chloramphenicol und Hygromycin als Hemmstoffe ermittelt. Aus Transformationen mit den mitochondrialen Transformationskassetten pBMhph II, pBMhph III und pBMhph IV konnten über 200 Hygromycin-resistente Linien selektiert und charakterisiert werden. PCR- und …
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Artificial Intron Technology to Generate Conditional Knock-Out Mice
… To test its utility for gene inactivation, a Hygromycin resistance plasmid containing Cre- recombinase was introduced into the intron-containing mESCs. A plus/minus screen for Neomycin resistance was performed and Sanger based sequencing was done to identify whether a conditional knock-out had …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… G5 HC-Pro. SMV HC-Pro enhanced the recovery of hygromycin-resistant somatic embryos from immature soybean cotyledonary explants co-cultured with Agrobacterium . Expression of SMV HC-Pro in transgenic plants partially stabilized the expression of a beta-glucuronidase (GUS) transgene that was …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… vectors and a plasmid conferring resistance to hygromycin-B (HYG-B), using a calcium phosphate precipitation procedure. Induction of the MMTV-LTR with the glucocorticoid dexamethasone (DEX) was required for enhanced expression of IGF-I in MD-IGF-I (MD=Mammary Derived) cells, whereas SV40-IGF-I …
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Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… fusion with plasmid DNA. The DNA contained a hygromycin B resistance gene (hph), which encodes a phosphtransferase, and is flanked by promotion and termination sequences from the trpC gene of Aspergillus nidulans. Colonies which grew from the initial selection were shown to be transiently …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… MX4 cassettes encoding resistance to phleomycin, hygromycin B, nourseothricin and bialaphos. Finally, in the process of generating the new plasmids, I have determined several errors in existing publicly available sequences for several commonly used yeast plasmids. Using updated plasmid sequences, …