Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"hydrophobic core"”.
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The role of hydrophobic core in protein folding
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1991.
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Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… peptide. Signal peptides contain a central core of uncharged amino acids that are usually hydrophobic in nature. The hydrophobic core of the parathyroid hormone (PTH) signal peptide is composed of 12 contiguous hydrophobic amino acids. Although the exact role of the core in signal peptide …
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Folding Studies on Mutants of Chymotrypsin Inhibitor 2
… kinetics of mutants at the helix N-terminus and hydrophobic core of Chymotrypsin Inhibitor 2 (CI2) have been studied. All mutants adhere to a two-state model for protein folding , and are destabilised relative to wild-type. Mutation of N-cap residue S31 to Ala or Gly destabilises CI2 by nearly 1 …
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Rational design of synthetic metalloproteins
… 3 explored the effect of systematically repacked hydrophobic core on both structural properties and metal-binding abilities of the resulting coiled coils. A series of coiled coils was designed to contain a similar Cys-X-X-Cys metal-binding site but different combinations of isoleucine/leucine …
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Deciphering Functional Significance of Substrate-Binding Domain of Ssa1 on Heat-Shock Response and Prion Propagation
… size of side chain contributes to maintain the hydrophobic core of SBD. Introduction of smaller amino acid side chains at residue 475, such as alanine and serine, resulted in temperature sensitivity and [PSI+] impairment. When the side chain of residue 475 is larger than Cysteine, no matter if …
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Investigation of Neural and Epithelial Cadherin Monomer Properties Using a Molecular Dynamics Approach
… residues at the N-terminus of the proteins, the hydrophobic core residues, and the ionic surface area.
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Heterotrimeric coiled-coils as viral fusion protein mimics
… of amino acids that serve to form both the hydrophobic core and electrostatic interfaces of the coiled-coil. Previous work in our lab showed the viability of designing a self-assembling heterotrimeric coiled-coil by sole manipulation of the hydrophobic core. This technique utilized a steric …
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Design and characterization of multicompartment micelles in aqueous solution
… is inherently limited to a homogeneous core surrounded by a corona, which keeps the micelle in solution. Thus, for drug-delivery applications, such structures only offer a single domain (the hydrophobic inner core) for drug entrapment. Whereas multicompartment micelles composed of a …
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Analysis of Molecular Dynamics Simulations of Protein Folding
… competition between local structure formation/hydrophobic collapse and global structure formation in all trajectories. Our analysis on the NLE trajectories confirms the notion that a tight hydrophobic core inhibits correct 3-D rearrangement. Only one of the six NLE trajectories folded, and it …
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Synthesis and study of the micellization behavior of a dual temperature- and pH-responsive PAA-b-PNIPAM block copolymer
… copolymers are composed of hydrophilic and hydrophobic regions. When they are exposed to water, the hydrophobic blocks aggregate to form a hydrophobic core surrounded by hydrophilic regions outside to give a core-shell micelle. These micelles have many potential applications, such as a drug …
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The synthesis of amphiphilic diblock copolymers: An investigation into the formation of micelles as a function of hydrophobic block length
… to their ability to aggregate into protective core-shell micelles. Using RAFT polymerization, copolymers containing poly(acrylic acid) and poly(methyl acrylate) blocks were made with high monomer conversion and narrow distributions of molecular weight for eventual use in medicinal applications. …
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Roles of cosolvents on protein stability
… by its destabilizing effect on the hydrophobic core: non-polar peptides and residues forming the hydrophobic core of the Trp-cage protein adopt more extended conformations in solutions containing TMAO. It is found that a main interaction that can stabilize folded proteins are …
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Biochemical and Structural Studies of Pseudouridine 55 Synthase
… the conserved tyrosine, which is part of hydrophobic core in the active site. The role of the conserved tyrosine is less obvious in addition to apparent structural role. Enzymatic activity assay reveals that mutating T. maritima Tyr67 to any other amino acids abolishes the enzymatic …
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Membrane fragmentation by a 20-29 fragment of human islet amyloid polypeptide and rat amyloid polypeptide
… of the bilayer by intercalating into the hydrophobic core. Last, dye leakage experiments indicate that both peptides induce membrane permeability, although a greater effect is seen for the hIAPP.</p>
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Structural Basis for Signal Transduction in LOV Blue Light Photoreceptors
… addressed intradomain signaling from the hydrophobic core to the domain surface. In this study, we addressed the role of a specific conserved residue in transmitting activating signal from the domain core to surface structural elements. Through biophysical and biochemical studies of LOV …
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Synthesis and Evaluation of Trans-bilayer Cross Linking Phospholipids Based on Click (Azide-Alkyne) Chemistry
… Formation of bola-PC were observed in the hydrophobic core of lipid bilayer and this was the main objective of this study.
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AB DIBLOCK COPOLYMER NANOPARTICLES VIA RAFT-MEDIATED POLYMERIZATION AS A PSEUDOSTATIONARY PHASE FOR ELECTROKINETIC CHROMATOGRAPHY
… character. Butyl acrylate was chosen for the hydrophobic core to stay consistent with previous work. Through the RAFT process, nanoparticles were successfully synthesized, demonstrating size control of both the hydrophilic block and hydrophobic block. The novel PSP performance was …
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AB DIBLOCK COPOLYMER NANOPARTICLES VIA RAFT-MEDIATED POLYMERIZATION AS A PSEUDOSTATIONARY PHASE FOR ELECTROKINETIC CHROMATOGRAPHY
… character. Butyl acrylate was chosen for the hydrophobic core to stay consistent with previous work. Through the RAFT process, nanoparticles were successfully synthesized, demonstrating size control of both the hydrophilic block and hydrophobic block. The novel PSP performance was …
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Preparation of Supramolecular Amphiphilic Cyclodextrin Bilayer Vesicles for Pharmaceutical Applications
… increases the ability to encapsulate hydrophobic pharmaceutical molecules by forming a carrier with a hydrophobic core and hydrophilic exterior. Guest molecules are commonly added to these inclusion complexes in order to add stability and further increase targeting abilities of the …
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Genetic Engineering of Functional Large Amyloid Fibers
… and serine (S)-containing P7S both have the same hydrophobic core, charge, and hydrogen bonding potential. However, P7Q is highly alpha-helical while P7S contains a beta-sheet core. After 72 hours, the Gd20:P7Q template:adder protein mixture produces tightly packed ~0.3 μm high and ~1.9 μm wide …
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