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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"hydrophilic interaction"”.
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An exploration of the mechanism of hydrophilic interaction chromatography (HILIC) and the application of HILIC in metabolomic profiling
The aim of this study was to investigate hydrophilic interaction chromatography (HILIC) and its application to metabolic profiling. The effect of different mobile phase modifiers on the retention behaviour of acidic and neutral test probes was studied on silica gel based columns which confirmed the …
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A study of the mechanism of hydrophilic interaction chromatography and its application in the analysis of medical drugs and metabolites
Hydrophilic interaction chromatography (HILIC) has been the subject of a few excellent reviews in recent years and most focused on different factors which impact on the separation of compounds in HILIC mode including column temperature, mobile phase composition, pH, buffer type and concentration. …
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Identification and enantioselective determination of protein amino acids in fertilizers by hydrophilic interaction and chiral chromatography coupled to tandem mass spectrometry /
… assay in HPF samples was developed in hydrophilic interaction chromatography (HILIC) mode with tandem MS/MS detection. The developed method delivers fast analysis and superior specificity. Enantioselective HPLC on a glycopeptide antibiotic teicoplanin-based chiral stationary phase …
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Synthesis & retention mechanisms of novel mixed mode stationary phase for detection of specific polar low molecular weight biological samples
… of novel mixed mode stationary phases for Hydrophilic Interaction Liquid Chromatography (HILIC). HILIC is a rapidly emerging chromatographic mode that is finding great applicability in the analysis of polar organic molecules. In addition, there is a chapter on the analysis of Bisphenol A …
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Development of a novel cyanide-free method for analysis of vitamin B₁₂ in milk-based infant formula
… The final analyte was quantified using hydrophilic interaction liquid chromatography with fluorescence detection. The single laboratory validation experiment showed that this cyanide-free method is fit for purpose. Analysis of various milk-based infant formulas and comparison with …
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Hybridization of PolyJet and Direct Write for the Direct Manufacture of Functional Electronics in Additively Manufactured Components
… on PolyJet substrates are measured and exhibit a hydrophilic interaction, indicating good adhesion. Encapsulation is found to negatively impact conductivity of directly written conductors when compared to traces deposited on the surface. Strain sensing components are designed to demonstrate …
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Metabolomics of cerebrospinal fluids to identity novel biomarkers as a predictive tool for brain inflammatory conditions
… within a twenty minute gradient elution using hydrophilic interaction chromatography. The method exhibited good reproducibility, high efficiency chromatographic separation and strong mass resolving mass spectrometry analysis. The practicality and robustness of the developed method on a pilot …
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Studies of UHPLC-MS performance and application to rapid sensitive and robust drug analysis
… affect the chromatographic performance of hydrophilic, basic analytes. Manipulation of mobile phase pH suppresses the protonation of basic analytes for improved retention, peak shape and resolution. This is exemplified by the ephedrines, which are inherently difficult to separate by …
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A study of the metabolism and metabolomics of polyphenols using isolated rat hepatocytes and liquid chromatography-high resolution mass spectrometry
… mainly converted to its sulfate. In addition, hydrophilic interaction chromatography in combination with high resolution mass spectrometry was used to study the effects of the phenolic compounds on the hepatocyte metabolome. Quercetin had a strong effect on the metabolome as it depleted the …
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Contact Angle Of A Nano-Drop On A Heterogeneous Surface
… of water and the location of surface-water interaction energy at the perimeter and beneath the drop. Young’s equations gives the relationship between surface tension, at the three phase solid liquid vapor interface, and contact angle on a homogeneous surface. Cassie and Baxter generalized …
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Optimising the Wider Energy Metabolome of Microbes Using Targeted UHPLC-MS/MS Present in Bio-Electrochemical Systems for Carbon Dioxide Conversion to Platform Chemicals
… in a single analytical run using Zwitterionic Hydrophilic Interaction Chromatography (Z-HILIC) coupled to tandem mass spectrometry. The resulting workflow offers high sensitivity, strong reproducibility, and low limits of detection and quantification, making it suitable for the analysis of …
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The Development and Application of Non- Targeted Mass Spectrometry Based Methods to Tea (Camellia Sinensis L.)
… methods were then developed; the first utilising hydrophilic interaction liquid chromatography to resolve mostly polar primary metabolites such as amino acids and nucleosides; the second using RP and ultra-high pressure liquid chromatography to maximise the chromatographic resolution of mostly …
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Purification and characterization of A[beta] and probing the interactions between myricetin and A[beta] using various spectroscopic methods
… spectroscopic studies suggest multiple forms of interaction between A[beta] and myricetin, a flavonoid compound. In this study, two A[beta] fragments, A[beta](1-16) and A[beta](25-40), together with A[beta](1-42) were investigated. HPLC purification was conducted on crude A[beta](1-16) and …
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Probing water properties and cationic exchange in calcium-silicate-hydrate : an atomistic modeling study
… with calcium. We showed that the nature of the interaction between the confined water and C-SH is hydrophilic. The interlayer calcium ions and the disorder in the silicate chains act synergistically to achieve this hydrophilic interaction. The water molecules ultra-confined in this hydrophilic …
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Synthesis and application of tagging agents in mass spectrometry-based targeted metabolomics studies
… of hexose sugars. The detection of the sugars by hydrophilic interaction chromatography (HILIC) in combination with mass spectrormetry (MS) was greatly improved along with chromatographic resolution allowing for easy identification of sugar isomers. In Chapter three, analysis of short chain fatty …
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Identification of the metabolites responsible for colour stability from different lamb sires and elucidation of the underlying biochemical pathways : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy in Biochemistry at Lincoln University
… groups. In metabolomics, using a normal phase hydrophilic interaction chromatography-mass spectrometry (HILIC-MS) approach, taurine, glutamic acid, aspartic acid and cytidine were identified. As a complementary measure to HILIC by using the reverse-phase C18 column in lipidomics, the approach …
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Cytostatics in the aquatic environment : analysis, occurrence, and possibilities for removal
… and 2’,2’-difluorodeoxyuridine. Hydrophilic interaction chromatography (HILIC), a chromatography for polar analytes in aqueous samples, was used to achieve a good retention of the polar analytes. Along with the method development, retention mechanisms on the HILIC stationary phase …
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Targeted and untargeted metabolomic profiling of human serum, urine and brain by liquid chromatography mass spectrometry (LC-MS)
… amination method was used in combination with hydrophilic interaction liquid chromatography and high resolution mass spectrometry to analyse the sugars in post-mortem human brain. The samples were collected from six control brain samples and eighteen samples from individuals who had suffered …
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Applications of LC-MS/MS in forensic toxicology for the analysis of drugs and their metabolites
… LC-MS method for these metabolites using a novel hydrophilic interaction liquid chromatography column was validated and applied to routine forensic casework. Ninety urine case samples were divided into three groups depending on the ethanol concentration found in blood and analysed by the developed …
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