Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"hydrolysis probes"”.
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Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens
… of 23 cases. Multiplex RT-PCR with four colour hydrolysis probes identified hrHPV genotypes in 22 of 23 cases with relative frequencies of HPV genotypes: 16>>18=39=45>33. Most cases showed infection with a single hrHPV genotype (HPV 16 and one case with HPV 33) with four cases demonstrating two …
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The Development of New Tools to Investigate Alphavirus Replication Kinetics
… We also determined that DNA hydrolysis probes increased the sensitivity of ssqPCR assays when compared to a double-stranded DNA-specific dye, SYBR Green. Using this information, we determined the replication kinetics of two different genotypes of o'nyong nyong virus (ONNV) and …
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Microbial Source Tracking of Bacteroides to Identify Human and Bovine Fecal Loading in McDaniel Lake
Fecal waste enters waterways from run-off during rain events, and is especially high in agricultural areas due to manure application and livestock. Increased fecal waste may also be due to direct human influence. This fecal loading may lead to eutrophication and poses health hazards to humans. By …
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Molecular detection and identification of Cryptosporidium species isolated from human and animal sources in Limpopo and Gauteng Provinces
… of 18S rRNA region; Real-time PCR employing Hydrolysis probes detection format for amplification of SSU rRNA region; Real-time PCR specific for amplification of C. hominis region and C. parvum region. Positive samples from real-time PCR that gave clear bands on gel electrophoresis were sent …