Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"hybridoma cells"”.
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Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems
… production by HPCHO Chinese hamster ovary cells and IB4 hybridoma cells in batch, fed-batch, and three perfusion systems: the stirred ceramic membrane reactor (SCMR), the alternating tangential flow (ATF) hollow fiber membrane system, and the external spin filter (ESF) system. The batch …
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Production of Monoclonal Antibodies for Toxoplasma gondii Lactate Dehydrogenase
… of T. gondii, at the first of all, hybridoma cell lines producing monoclonal antibodies with high affinity and specificity against T. gondii LDH (TgLDH) were established. Methods: After 6-8 week old BALB/c mice were immunized with synthetic peptides of TgLDH, splenocytes were …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… BL (HBL) and non-hemolytic enterotoxin (Nhe) on cells. For that purpose the cytotoxic and hemolytic activity of crude B. cereus culture supernatants was analyzed in cell culture assays and on blood agar plates. Specific monoclonal antibodies were used for the detection of single components. By …
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Establishment of a systematised approach for the development of novel diagnostics and therapeutics in the field of biotechnology
… mouse serum. Successful fusion of the murine B-cells with myeloma cells were performed followed by single cell sorting (FACS). Monoclonal clones could be established and at least 4 stable cell lines showed specific binding to Nonylphenol in ELISA. One antibody was further tested in a ligand …
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Untersuchung von antigenpräsentierenden Funktionen bei kultivierten hepatischen Sternzellen und transdifferenzierenden Myofibroblasten der Ratte
Studies with liver cells in mice have shown that even cells that are not directly attributed to the immune system can play an antigen-presenting function. The aim of this study was to investigate the behavior of these antigen-presenting hepatic stellate cells (HSC) in rat. Also changes of the …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… I generated and purified antibody from two Hybridoma lines; NG3.5 and VE-4. To generate enough antibody for immunological assays, I grew large quantities of antibody-producing hybridoma cells and collected the supernatant. From this, I isolated and purified the antibody for future assays. We …
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Developing Methods to Study Pathogenesis and Immune Response to HSV-2 in the Guinea Pig Model
… I generated and purified antibody from two Hybridoma lines; NG3.5 and VE-4. To generate enough antibody for immunological assays, I grew large quantities of antibody-producing hybridoma cells and collected the supernatant. From this, I isolated and purified the antibody for future assays. We …
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Wechselwirkungen von Immunzellen mit synthetischen und biomimetischen Oberflächen
Die vorliegende Arbeit wurde im Zeitraum von Oktober 2002 bis November 2005 an dem Institut für Biochemie und Biologie der Universität Potsdam in Kooperation mit dem Institut für Chemie des GKSS Forschungszentrums in Teltow unter der Leitung von Herrn Prof. Dr. B. Micheel und Herrn Prof. Dr. Th. …
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Development of recombinant antibody mediated resistance against Tomato yellow leaf curl virus
… expression of specific scFv fragment in plant cells for suppression of disease symptoms caused by TYLCV infection. The C1, V1 and V2 genes encoding Rep, CP and MP, respectively, were cloned into the TOPO vector and subsequently into pGEX-5x3 and pMALc2x expression vectors. Recombinant proteins …
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Antigen-specific depletion of autoreactive B lymphocytes in Multiple Sclerosis
… complement system. Moreover, autoreactive B cells can strongly enhance the T cell response by presenting processed autoantigens in combination with MHC molecules on their surface. In this thesis, novel autoantigen-specific fusion proteins for the targeting and elimination on one hand and the …