Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"hybridization probes"”.
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Dynamic DNA Nanotechnology for Probing Single Nucleotide Variants and DNA Modifications
In the last decades, various DNA hybridization probes have been developed that attempt to conquer the challenge of single-nucleotide-variants (SNVs) detection. Even though a powerful toolbox including the toehold-exchange reaction, the dynamic ‘sink’ design, and the polymerase chain reaction (PCR) …
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Structural and functional analysis of anaerobic biofilm communities: An integrated molecular and modeling approach
… were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. Using these probes, it was determined that Methanosarcina-species, Methanobacteriales, and Methanosaeta-species were the most abundant methanogens present in …
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Oligonucleotide Design for Whole Genome Tiling Arrays
… microarrays, or FISH (fluorescence in situ hybridization) probes. While traditional microarrays are commonly used for measuring gene expression levels by probing for sequences of known and predicted genes, high-density, whole genome tiling arrays probe intensively for sequences that are …
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Studying Tissue Specific Diversity of Splicing Regulator Muscleblind
… interest. Finally, we used fluorescent in situ hybridization probes to characterize expression of alternatively spliced exon transcripts across differentiation adult drosophila tissue. We found robust mbl expression in the central nervous system and variable exon presence in visceral and …
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Optimization of Molecular Beacon-Based Multicomponent Probes for Analysis of Nucleic Acids
… for recognition of SNS takes advantage of hybridization probes that bind target nucleic acids followed by measuring ?Tm, the difference in melting temperatures of matched and mismatched hybrids. The approach enables SNS differentiation at elevated temperatures (usually 40-65oC) often only …
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Geobiology of marine magnetotactic bacteria
… in their 16s rRNA. Fluorescent in situ hybridization probes showed significant variation in clade abundances across a seasonal cycle in salt marsh productivity. FISH also showed that aggregates consist of genetically identical cells. QPCR data indicated that populations are finely …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
New luminescent probes with enhanced brightness convenient for bioconjugation were synthesized and tested in major biological applications including the detection of nucleic acids and living cells. The first project used luminescent lanthanide ion probes that take advantage of hyper-sensitive …
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Filamentous Foaming in Activated Sludge Systems: A Study Combining Molecular and Engineering Approaches
… of ribosomal RNA (rRNA)-targeted oligonucleotide hybridization probes allows for the reliable identification and quantification of specific microbial populations. Group-, genus-, and species-specific small subunit rRNA-targeted oligonucleotide probes for the mycolata, Gordonia, and Gordonia …
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Using Molecular Signature Methods to Measure the in Situ Physiology of Acinetobacter Spp. In Enhanced Biological Phosphorus Removal Activated Sludge Wastewater Treatment
… biological phosphorus removal. Oligonucleotide hybridization probes targeting the 16S ribosomal ribonucleic acid of various phylogenetic groups of microorganisms including the beta-subclass Proteobacteria, gamma-subclass Proteobacteria, the genus Acinetobacter, Acinetobacter johnsonii, …
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Characterization of cytoplasmic diversity in soybean (Glycine max L. Merr) using mitochondrial markers
… used to distinguish cytoplasmic groups based on hybridization analysis of <i>Hind</i>III-digested soybean DNA Four major groups were observed with the 2.3 kb marker in the old domestic collection, and several minor subgroups were also detected. The second objective included subcloning and …
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A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases
… coding region was identified by using cosmids as hybridization probes to detect chromosomal alterations in the DNA of bli-4 mutant strains. Two mutations of bli-4 are small rearrangements of the gene; e937, is a 3.5 kilo base (kb)deletion, and h1010 is an insertion of the 1.6 kb transposable …
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Ionic Self-Assembled Multilayers in a Long Period Grating Sensor for Bacteria and as a Source of Second-Harmonic Generation Plasmonically Enhanced by Silver Nanoprisms
… streptavidin were used to attach biotinylated hybridization probes specific to distinct species of textit{Brucella}. The sensor was then exposed to lysed cell cultures and tissue samples in order to evaluate its performance. The best results were obtained when using samples from …
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Molecular And Therapeutic Implications Of Notch1 Signaling In Pediatric T-Cell Acute Lymphoblastic Leukemia
… Using real-time PCR techniques with specific hybridization probes, we were able to determine that the leukemic clone for one patient was present at the time of diagnosis, but at a very low expression level. This suggests that the clone responsible for relapse was resistant to the initial …