Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"humanized mice"”.
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Mucosal HIV-1 Transmission in Humanized Mice
… HIV-1 transmission, the susceptibility of humanized mice to intrarectal and intravaginal HIV-1 infection was investigated. Human lymphocytes, including CD4+ T cells, generated in situ from hematopoietic stem cells reconstitute the gastrointestinal tract and the female reproductive tract of …
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Modeling hematologic malignancies and their treatment in humanized mice
… Yet, as our understanding of cancer in mice grows more sophisticated, it is important to be aware of vital differences between murine models and human patients, differences in basic physiology, immunology, and in susceptibility to cancer itself. Furthermore, as anti-neoplastic therapy …
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Humanized mice to test vaccination against influenza virus via dendritic cells.
… Here,we used NOD-SCID-β²m-/- immunodeficient mice which, when engrafted with human CD34⁺ hematopoietic progenitors, develop all subsets of human dendritic cells(DCs) and B cells. T cells and their subsets were reconstituted by adoptive transfer. We found myeloid DCs, plasmacytoid DCs and …
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Modeling dengue viral infection, insect transmission and multiplex PCR in humanized mice
… response. In this regard, new advances with humanized mice(hu-mice), constructed by engrafting human tissues and cells and harboring a functioning human immune system, have been of great value in advancing research with human viruses Here we sought to utilize hu-mice to understand natural …
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Therapeutic Uses of Broadly Neutralizing Anti-HIV-1 Antibodies in Humanized Mice
… and diversifies at the expected rate within humanized mice (hu-mice) and that hu-mice can sustain HIV-1 viremia for up to 4 months, thus allowing hu-mice to be used as a small animal model to study HIV-1 therapeutics. Using hu-mice, I show that when administered as single agents, recently …
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Lethal Hypermutation Induced by the Innate Cellular Restriction Factor APOBEC Destroys HIV-1 in Infected Humanized Mice
… able to evade APOBEC restriction in vivo exists. Humanized mice used as an in vivo model to study the capacity of APOBEC to restrict Vif-defective HIV revealed that the ability of the virus to replicate is severely crippled. HIV lacking Vif is heavily hypermutated by APOBEC, however, if the virus …
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To Investigate The Impact Of Gut Bacteria On Efflux Transporter Expression And Function In Gastrointestinal Mucosae
… intestinal organoids derived from germ free and humanized mice. Typically, transport across the epithelial cell layers occurs by active transport and passive diffusion. Using mouse models that differ in bacterial content and fluorescence scanning, and live imaging techniques we studied the impact …
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Humanized Mouse with a Diverse Polyclonal B Cell Repertoire as a Model for Neutralizing Antibody Responses against EBV and HIV
… project, I investigated the utility of these humanized mice as a surrogate to model VRC01-class B cell responses, a rare class of HIV broadly neutralizing antibodies that target the CD4 binding site on HIV envelope. I utilized high-throughput sequencing to identify the presence of these rare …
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Of Mice and Men: Studying Innate and Adaptive Immunity Against the Epstein-Barr Virus
… with EBV by reconstituting NOD-scid γc-/- mice with CD34+ hematopoietic stem cells. We demonstrated that primary T cell responses in these humanized mice control infection with EBV. These T cell responses were HLA restricted and partially specific for EBV derived peptides. In HLA-A2 …
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AXL Targeting Restores PD-1 Blockade Sensitivity of STK11/LKB1 Mutant NSCLC Through Expansion of TCF1+ CD8 T Cells
… in syngeneic immunocompetent mouse models and in humanized mice bearing STK11/LKB1 mutant NSCLC human tumor xenografts. Anecdotal NSCLC patients with STK11/LKB1 mutant tumors also demonstrated responses to the combination of AXL inhibitor bemcentinib and pembrolizumab. We conclude that AXL is a …
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Putative Roles of Cd200 In The Leukemogenesis and Immune Evasion of Leukemia Stem Cells
… a physiological setting, we established a PBMC-humanized mouse model, in which we could characterize progression of CD200+ and CD200- AML in the presence of an activate human immune response. Further, we used this model to compare the effects of CD200+ AML on the transcriptional activity and …
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Inhibition of adenosinergic signaling in the tumor immune environment: the effect of combination CD73 blockade and oHSV therapy on solid tumors
… models. Tumor-bearing CD73 global knock-out mice treated with oHSV and wild type mice treated with a murine CD73 antibody and oHSV showed significant improvements in therapeutic efficacy of oHSV. Single cell RNA sequencing was employed to assess changes in immune cell infiltration and …
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Mechanisms of Innate Immune Dysfunction During Mycobacterium tuberculosis and HIV Co-infection
… lung. Pulmonary macrophages from MGL-1-deficient mice infected with Mtb displayed increased pro-inflammatory cytokine levels (IL-1β, IL-6, and IFN-g) associated with significant lipid accumulation. Surprisingly for a CLR, we also observed MGL-1-dependent anti-mycobacterial activity, evidenced by …
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Development of a Humanized Mouse Model to Understand M.tb and HIV/M.tb Co-infection
… The goal of these studies is to develop the humanized BLT mouse as a small animal model to study HIV/M.tb co-infection and identify mechanisms of HIV-mediated immune dysfunction that alter the protective response against M.tb infection. Humanized BLT mice are infected with M.tb and develop …
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Study of Natural Killer Cells and Their Therapeutic Role in Pediatric Cancer
… differences of NK cell subsets in these mice compared to patient NK cells, the humanized MISTRG neuroblastoma model is a valuable tool to study NK cell biology in neuroblastoma and test whether therapeutic interventions, such as cytokine supplementation, can overcome impaired NK …