Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"human stem cells"”.
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Human Stem Cells for Modeling Amyotrophic Lateral Sclerosis Disease Mechanisms and Modifiers
… a fatal neurodegenerative disease of the motor system. Although ALS has been extensively studied in post-mortem patient samples and animal models, there are currently no very effective treatments and there is no cure. One reason for the lack of treatment options in ALS may stem from the …
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Phenotypic analysis of human stem cells and their differentiation potential towards pancreatic cells
… is an autoimmune disorder in which the immune system specifically destroys the insulin-producing β-cells of the pancreas, resulting in a loss of glycemic control. There is no cure for the disease, and patients have the difficult task of managing their blood glucose levels by daily injection of …
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ESTABLISHMENT IN CULTURE OF MOUSE AND HUMAN STEM CELLS WITH EXPANDED FATE POTENTIAL
… cell lines that resemble these totipotent-like cells. In this work, I hypothesised that by modulating signalling pathways known to be important in early embryonic development it may be possible to capture in vitro a self-renewing state that possessed features of pre-implantation blastomeres. I …
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Genome-Wide and Multi-Scale Mapping of DNA Supercoiling in Differentiating Human Stem Cells
… of every cell is encoded in its genome. Human cells must organise and compact their genetic material at multiple levels to accommodate the two-meter-long DNA within the confined nuclear space of six microns. Chromatin folding is not only a cellular need, but is also crucial for gene …
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DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
During differentiation, pluripotent cells must commit to one of many possible lineages. This commitment is mediated by RNA polymerase II (Pol II), which is recruited by transcription factors, guided by enhancers, excluded from heterochromatin, and repositioned through DNA looping. While each of …
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Patient Experience Living with Split Thickness Skin Grafts
… to participate in future clinical trials using human stem cells.
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United States stem cells research boundaries
… of researchers. We focus our investigation on stem cell research in the United States (US). Research in human developmental biology has led to the discovery of human stem cells. The science of stem cell therapies is about to enter a phase of research and development that could lead to …
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Manufacturing and engineering of therapeutic extracellular vesicles
Originally viewed as 'garbage bags' which cells release to dispose of unwanted material, extracellular vesicles (EVs) have emerged as potent messengers that package and disseminate biochemical signals. This newly recognized mode of communication between cells has brought unprecedented therapeutic …
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Islamic Bioethics: National Regulations and Guidelines of Human Stem Cell Research in the Muslim World
<p>The utilization of human stem cells emerged recently in the Muslim world as one of the essential valuable areas of medicine for their vital role in developing regenerative medicine and treating chronic and incurable diseases. Existing studies indicate that most human stem cell researchers rely …
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Characterization and application of human pluripotent stem cell-derived neurons to evaluate the risk of developmental neurotoxicity with antiepileptic drugs in vitro
… risks of damage to the developing nervous system of many chemicals are not known because these studies often require costly and time-consuming multi-generational animal experiments. Pluripotent stem cell-based systems can facilitate developmental neurotoxicity studies because disturbances in …
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Effects of prolonged mitosis on neural stem cells in vivo during development
… 12 microcephaly-linked genes identified in human genetics studies encode microtubule/centrosome-associated proteins and mutations in these genes are strongly tied to disrupted mitotic processes in neural stem cells during cortical development. Yet, how perturbed neural stem cell mitosis …
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Targeting the hematopoietic stem cell to correct osteopetrosis
This thesis focuses on developing stem cell targeted gene therapy for the severe hereditary disorder Infantile Malignant Osteopetrosis (IMO) as well as increasing the understanding of how the genetic defect present in IMO affects the hematopoietic stem cells and hematopoiesis in general. IMO is a …
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Modeling hematologic malignancies and their treatment in humanized mice
… are due to neoplasms of the hematopoietic system, such as leukemias and lymphomas. Genetically engineered mouse models of these diseases have yielded invaluable insights into the ways normal processes become corrupted, leading to cancer development, progression, and spreading. Yet, as our …
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Engineering novel neuronal circuits using HI-PSCS derived neurons and microglia
… will remodel the connections within the human cortical neuronal circuit development, resulting in higher synchronicity and stable firing of action potentials for a longer period of time. The presence of micro-glia could also help the neuronal circuits and systems to reach maturation …
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Molecular Basis of Mechanosensitivity
… of sources including numerous cancer cell lines, human stem cells and mouse stem cells. Further work using transcriptome analysis, bioinformatic techniques, and electrophysiological recordings identified that the pore forming subunit responsible for the slowinactivating mechanosensitive …
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Using yeast to study neurodegenerative diseases : amyloid formation as a protective mechanism and a new Alzheimer's disease model
… of protein damage during the mitoses of cells ranging from E. coli to human stem cells. Yeast prion proteins are another group of proteins capable of adapting an amyloid conformation. The self-templating amyloid fold allows yeast prions to act as non-Mendelian elements of inheritance. We …
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High-throughput approaches to sourcing of human hepatocytes for cell-based therapies
… therapies. Such therapies should ideally employ human cells due to immunological concerns and the substantial differences between animal and human hepatocellular functions. Human cell lines, while renewable, lack the full functional capacity of primary adult hepatocytes, and for clinical …
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Development of an in vitro human lung organoid system to study alveolar type 2 stem cell maintenance and dysfunction during disease
The human adult distal lung parenchyma is maintained by alveolar type 2 (hAT2) cells that have the ability to self-renew and differentiate into alveolar type 1 (hAT1) cells, a critical cell type involved in gas-exchange. Cellular damage within the alveoli can lead to severe lung dysfunction, and …