Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"human samples"”.
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Studying Models of the Amyloid Cascade Hypothesis Using Single-Molecule Imaging
… While these methods were previously applied to human samples, they have not been used to study disease models. Cellular and animal models have numerous advantages over human samples, primarily less intra-group variation and the ability to manipulate individual pathways. Thus, these models enable …
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Epigenetic Regulation of Neurogenesis in Non-Demented Humans with Alzheimer’s Disease Neuropathology
… of the hippocampus in 4 distinct populations of human subject’s tissue representing the full disease, intermediate cognitive decline, healthy controls, and a poorly characterized group who have all of the histopathological hallmarks of the full disease but are cognitively normal. To investigate …
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Characterisation of neurodegenerative diseases derived protein aggregates using improved single-molecule pull-down
… these protein species in the complex human samples and How to find the key species associated with these diseases. To address these questions, this thesis begins by establishing an improved single-molecule pull-down (SiMPull) assay, which was used to characterise soluble α-Synuclein …
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Quantification of benzo[a]pyrene-diol-epoxide adducts by laser-induced fluorescence spectroscopy
Quantification of adducts in human samples is a challenging task in analytical chemistry. Humans are typically exposed to low levels of a wide range of different carcinogens. As a result, adduct quantification requires both a highly sensitive and a highly selective method. This project has focused …
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Profiling, prototyping, and perturbing human immune responses
… and manipulation of limited quantities of human samples, shifting focus away from inbred mice and allowing an alternative research framework to emerge. This approach aims to ‘reverse-engineer’ the human response by finding disease-relevant biological phenomena through deep phenotyping of …
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Collagen-mimetic peptides for diagnosis and analysis
Collagen, the most abundant protein in the human body, is an essential scaffold for tissue development, regulation, and homeostasis. As a major structural component of the extracellular matrix, collagen is not static. Rather, it is highly diverse and dynamic, actively participating in tissue …
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Single-cell differential splicing of Alzheimer’s Disease in 1.9 million cells across 416 individuals
… prefrontal cortex of 416 post-mortem human samples to model temporal regulatory changes of AD. We use RNA velocity to infer spliced and unspliced counts for each gene and a beta-binomial distribution to model the unspliced/spliced ratio, and we identify differentially-spliced genes …
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Effects of dietary components on antibiotic resistance using mechanistic and plasmid based studies
… may have the potential to spread from animals to humans through the food chain. It has been observed that prebiotics such as mannan rich fraction (MRF) improve broiler growth and performance in a similar manner to antibiotic growth promotors. They have also been linked to a reduction in antibiotic …
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Estimating telomere length from whole genome sequencing data
… count or size of the genome within input samples. This means that Telomerecat lends itself well to analysing cancer samples where such assumptions are unfounded. This also means it is applicable to non-human samples, a first for tools of its kind. Furthermore, a novel method for filtering …
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Profiling Host Respiratory Responses to SARS-CoV-2 Infection
… animal models of infection, and profiling human samples with our clinical collaborators, all the while adding to the vast trove of COVID-19 knowledge generated amidst a global pandemic. In the early days of the pandemic, we identify putative targets of SARS-CoV-2 infection in human and …
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Expression and Regulation of Human Cytochrome P450 4F Isoforms In Tissue Samples and Under Tnf-Alpha Challenges
… CYP4F isoform expression profiles in a number of human samples when compared to the average population. The CYP4F isoform expression levels change with the degree of inflammation present in tissue. Further investigation in cell culture studies revealed that inflammatory cytokines, in particular …
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Optimisation of DNA extraction from teeth submerged in sea water in False Bay, South Africa
Extracting forensically useable DNA from human remains recovered from an open marine environment is problematic, and in some cases, impossible. The reason is unclear given the lack of research on marine decomposition, DNA survival in seawater, and possible methods to optimise the DNA extraction …
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Characterizing the Role of TLX1 in T-cell Acute Lymphoblastic Leukemia
… stages of oncogenic transformation. TLX1 T-ALL human samples translocated TLX1 to the TCRδ locus and had diverse secondary mutations, and TLX1 strongly colocalized with the BAF complex and chromatin loop anchors. Our mouse model of TLX1 T-ALL revealed the transformation of pre-leukemic cells …
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CD4+ T cell response against food antigens in intestinal disease
… memory T cells specific for food antigens in human samples. Comparison of food-reactive CD4+ T cell responses, especially against wheat, between healthy donors and patients with IBD, identified novel mechanisms of how oral tolerance can be broken. This work demonstrated that CD4+ T cells …
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Detection of trace levels of circulating tumour DNA in early stage non-small cell lung cancer
… published methods. INVAR was applied to samples from 90 treatment naïve patients with early stage non-small cell lung cancer to characterise the ctDNA levels and could provide a better sensitivity than comparable cohorts. In addition, I outsourced samples from 27 patients to be analysed …
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Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes
… real-time PCR assay to detect Bartonella spp. in human samples. For this study, we constructed a plasmid vector containing an insert of 83bp, derived from the Bartonella nuoG gene. In this non-clinical, laboratory evaluation, we used one laboratory sample to amplify the nuoG bacterial DNA fragment …
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4D mapping of network-specific pathological propagation in Alzheimer's disease
… showing disease-specific A[beta] aggregation in human samples from the early hub regions. Together, this data unites desperate observations of network-level deficits and identifies critical locations of early A[beta] deposition in the brain. By linking molecular and network observations, I begin …
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Unveiling Immune Checkpoint Therapy Resistance Mechanisms In The Tumor Microenvironment Using High-Dimensional Analyses
… Gene 6 (TSG-6). Our findings in murine and human samples suggest that TSG-6 interacts with CD44+ myeloid cells in the TME, contributing to their suppressive phenotypes. High TSG-6 levels correlated with poor prognosis in patients treated with ICT, and <em>in vivo</em> inhibition of TSG-6 in …
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Sensitivity and Species Specificity of Bisulfite Modification and Pyrosequencing Technology Used to Identify Tissue-Specific DNA Methylation Patterns in Common Biofluids Foudn in Crime Scenes
… test the sensitivity of these technologies are human specific, or if the primers will cross react with non-human biological materials. Therefore, the three previously mentioned markers were studied for their species specificity. After bisulfite modification, PCR amplification, and …
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Intake, growth and rate of digesta passage in ruminating calves fed sodium bicarbonate and disodium phosphate
… Animals were on a growth trial from wk 7 to 14. Human samples were taken for determination of pH, buffering capacity, volatile fatty acids anct ruminal ammonia and blood samples for determination of glucose and plasma urea nitrogen at 8, 11 and 14 wk. Rate of passage was determined using …
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