Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"human proteome"”.
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Novel Applications of Top Down MS/MS for Interrogating the Human Proteome
Top Down MS analysis of proteins from human cervical carcinoma cell lines and primary human leukocytes harvested from leukoreduction filters provided identification of 261 protein forms, with ∼12% overlap between the two proteomes. The protein forms were produced by 162 unique genes (∼22% …
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How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH
Protein-protein interactions form the backbone of the myriad signaling pathways that cells use to respond to their environments. A central class of protein-protein interactions in eukaryotic interactomes are those that occur between short linear motifs (SLiMs) and structured domains. SLiMs are …
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Computational Methods to Identify and Target Druggable Binding Sites at Protein-Protein Interactions in the Human Proteome
… a census of cancer-specific binding sites in the human proteome are identified to provide opportunities for drug discovery at the system level. Finally, tumor genomic, protein-protein interaction, and protein structural data is integrated to create chemogenomic libraries for phenotypic screening …
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Protein structural and functional consequences of missense mutations in the human cancer genome
… allowing researchers to scrutinise the human mutational landscape with an incredibly high coverage. As these resources grow, new variants get incorporated into the pool and older variants get updated annotations, further refining the quality of the information stored. Sometimes, however, …
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Sequence-Based Properties that Identify Intrinsically Disordered Phase-Separating Protein Regions
… secondary structures. While at least 40% of the human proteome is classified as IDPs, only a subset exhibit phase separation behavior. Previous work created a computer algorithm called ParSe (partition sequence) that successfully predicts folded, ID, and phase-separating (PS) IDRs from the …
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A Nascent Peptide Code for Translational Control of mRNA Stability in Human Cells
… motifs that predictably alter mRNA stability in human cells remain poorly defined. Here, we develop a massively parallel assay to measure mRNA effects of thousands of synthetic and endogenous coding sequence motifs in human cells. We identify several families of simple dipeptide repeats whose …
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MOLECULAR MECHANISMS OF ATP-MODULATED LIQUID-LIQUID PHASE SEPARATION (LLPS) OF TDP-43 AND SARS-COV-2 NUCLEOCAPSID PROTEIN
An estimated 40% of human proteome has undergone LLPS; delineating its molecular mechanisms may reveal principles undiscovered in physiology and open avenues for pathology. All living cells inexplicably maintain 2-14 mM ATP. Strikingly, in 2017, ATP (> 5 mM) was demonstrated to behave as a …
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Determining and characterizing immunological self/non-self
… and non-self peptides. The sequencing of human, mouse and numerous pathogen genomes and cataloging of their respective proteomes allows host self and non-self peptides to be identified. T-cells make this determination at the peptide level based on peptides displayed by MHC molecules.In …
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An interdisciplinary study of the mechanical and dynamic properties of α-solenoid repeat proteins
… yet they comprise nearly one third of the human proteome and are central to many cellular processes and disease phenotypes. Repeat proteins have been shown to behave like nano-sized biological springs: they are flexible, dynamic and elastic. Using coarse-grained models, I discuss how …
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Targeting Ph Domain Proteins For Cancer Therapy
… are one of the biggest protein families in the human proteome. However, no drugs have been achieved to the late development stages, let alone getting to the market. Thus, a deeper understanding of this protein family is required and there is an urgent need to develop novel small molecule …
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Computational structure analysis and prediction of Ser/Thr modified by O-GlcNAc in human proteins
… were applied to around 2.5 million S/T in the human proteome. Nuclear and cytoplasmic protein were over-represented among the top ranking proteins. Top scoring sites were also more likely to be phosphorylated. Also, novel and potential proteins were identified within the predictions.
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Prediction and Investigation of Novel Proteins in DNA Double Stranded Break Repair
… novel PPIs between DNA repair proteins and human proteome. Using this method, we predicted 271 novel PPIs, expanding the reported human DNA repair interactome by 75%. Yeast homologs of the novel human gene candidates were subjected to a plasmid-based repair assay, in which deletion of eight …
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Small Molecule Binding to Disordered Proteins
The human proteome includes many proteins that are entirely disordered or contain long disordered regions. Although these proteins are linked with many diseases, there are currently no clinically-approved drugs that have been shown to target these proteins in their monomeric forms. In fact, …
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Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing
… a synthetic representation of the complete human proteome, which has been engineered for display on bacteriophage. We use this library together with deep DNA sequencing methods to profile the autoantibody repertoires of individuals with autoimmune disease in a procedure called phage …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… adhere to the three rules. A search of the human proteome revealed a striking enrichment of chromatin-binding proteins, and we termed these H3 N-terminal mimicry proteins (H3TMs). We selected seven H3TMs and synthesized the methylated forms of their N-termini. Using a methyl reader …
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Comparative phylogenetic exploration of the human mitochondrial proteome: Insights into disease and metabolism
Mitochondria are a key organelle within human cells, with functions ranging from ATP synthesis to apoptosis. Changes in mitochondrial function are associated with many diseases, as well as ‘natural’ processes like ageing. Mitochondria have a unique evolutionary origin, as the result of an …
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Kinetics of Disordered Proteins and their Interactions
… proteins and regions are highly prevalent in the human proteome, and are often implicated in disease. However, methods to study these systems in detail are lacking, and the potential for thermodynamic and kinetic characterisation using experimental methods is limited. Molecular simulations and …
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Differential Impact of VEGF and FGF2 Signaling Mechanisms on Flt1 Pre-mRNA Splicing
The human proteome is exponentially derived from a limited number of genes via alternative splicing, where one gene gives rise to multiple proteins. Alternatively spliced gene products, although crucial for normal physiology, are also linked to an increasing number of pathologies. Consequently, a …
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Origins and composition of the cullin-RING E3 ligases in trypanosomes
… for ubiquitylation of up to 20% of the human proteome. In contrast with the rest of the class, cullin-RINGs perform their function as multi-subunit complexes that comprise substrate adaptors, RINGs for the transference of ubiquitin and cullins that serve as scaffold for the …
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A Mutated Peptide Database for the Analysis of Proteomic Mass Spectrometry Data
… resource that contains mutated peptides of the human proteome. This process resulted in a new database, termed XMAn, comprising 3,793,617 missense and nonsense mutations. The XMAn v3 database was used to identify mutations in the MDA-MB-231 triple negative breast cancer cell line. A total of 540 …
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