Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"human protein C"”.
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Expression of Human Protein C in Transgenic Tobacco
Human Protein C (hPC) is a vitamin K-dependent serine protease that has a critical role in the naturally-occurring anticoagulant pathway. Upon activation of the zymogen by thrombin at the endothelial cell surface, the active form of hPC has anticoagulant activity in hemostasis due to its ability to …
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Potential sources for the large scale production of human protein C
The vitamin K-dependent family of proteins (VKDs) include prothrombin, factors VII, IX, and X, and protein C (hPC) is synthesized in the liver and act to maintains normal hemostasis. such as properly regulated clotting. An imbalance of any of these pro- or anti-clotting proteins result in …
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Improvement of expression of recombinant human protein C in the milk of transgenic animals using a novel transgene construct
… of transgenes using the murine whey acidic protein (WAP) promoter have shown widely variable, position-dependent and copy number-dependent expression. This study evaluates a series of three WAP transgenes containing the cDNA of human protein C (hPC) for the expression of human protein C in …
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Optimization of metal dependent antibodies for chromatography
… the "Ca²⁺ -dependent" HPC4 are directed against human Protein C (PC). The 7D7BI0 antibody was characterized in terms of its metaldependency and specificity. The region of PC (epitope) recognized by 7D7BlO was identified as the first 15 residues in the NH₂-terminal. Immunosorbents made with 7D7BI0 …
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Delipidation Treatments for Large-Scale Protein Purification Processing
… insoluble. Lipid removal is desired prior to protein purification processing to decrease nonspecific fouling of downstream chromatographic matrices. Transgenic pig milk was used as a model system to study delipidation from therapeutic protein sources. The majority of triglycerides was …
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Downstream processing of recombinant and endogenous proteins from livestock milk
With the increased demands of therapeutic proteins, there is going to be a need for new purification technologies which have high throughput, high yield and high resolution. Three purification technologies were explored as potential new technology to isolate recombinant and endogenous milk …
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Design of anion exchange cellulose hydrogel for large proteins
… defined a minimum of two different types of protein binding site architecture. DEAE cellulose beads exhibited molecular exclusion of BSA near the edge of the bead in contrast to greater permeability seen in underivatized beads. Thus, accessible BSA binding sites are present only on the …
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Optimization of large beaded cellulose as a chromatographic support
… beaded adsorbent materials for column-mode protein purification has emphasized the impact of diffusional transport phenomena upon adsorbent capacity. A design model is presented that optimizes molecular accessibility of proteins relative to the mechanical stability of the material by …
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Identification of the factors impacting immunosorbent performance
… a vital role in the purification of therapeutic proteins. In most cases immunoaffinity chromatography is used as the final clean up step to yield a highly pure product. The customized avidity of the parent antibody for a given antigen or hapten can make immunoaffinity chromatography an …
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Effect of culture conditions, donor source, and injection site on in vitro development of deoxyribonucleic acid microinjected porcine zygotes
… by PCR is possible, but cross specificity of human Protein C and whey acidic protein (WAP) oligonucleotides for endogenous porcine DNA is strong and creates difficulty in applying PCR analysis to embryos microinjected with WAP-PC transgenes.
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Factors affecting the efficiency of gene transfer in mice
… h after hCG. The gene used was the whey acidic protein promoter linked to a coding sequence of the human protein C gene (WAP-hPC). Embryos were cultured in CZB at 37°C in 5% CO₂ in air. All the live pups born and embryos and fetuses recovered were analyzed by the polymerase chain reaction to …