Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"human pluripotent stem cells (hPSCs)"”.
-
The Derivation of the Myocardial, Epicardial and Endocardial Lineages from Human Pluripotent Stem Cells
The adult heart is composed of cells from three major lineages: the myocardium, the epicardium and the endocardium. The myocardium is the working muscle and comprises primarily the ventricles and atria. The epicardium represents the outer epithelial layer that surrounds the myocardium and plays a …
-
SGK196 controls stem cell fates by promoting the degradation of TGFβ family receptors
… regarding the molecular mechanisms controlling stem cell fate. Through studies in human pluripotent stem cells (hPSCs), I have identified SGK196, an atypical kinase of relatively unknown function, as a novel regulator of cell fate and the TGFβ family signaling pathway. SGK196 depletion in human …
-
Approaches to Reduce Selection of Genomic Variants in Human Pluripotent Stem Cell Culture
… conditions that reduce genomic instability in human pluripotent stem cells (hPSCs) is an unmet challenge in the field. Results from our lab and numerous research groups demonstrate that hPSCs are prone to genomic aberrations and single-cell passaging increases the rate of genomic alterations. …
-
The Maturation of Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells and Their Degeneration in Glaucoma
… leading to the degeneration of retinal ganglion cells (RGCs) and eventual blindness. A need exists to better understand the maturation of human RGCs as well as their degeneration, with the goal of developing new therapeutics diseases like glaucoma. Human pluripotent stem cells (hPSCs) provide an …
-
Generation and characterisation of primary and pluripotent stem cell-derived human hepatoblast organoids
Use of human pluripotent stem cells (hPSCs) has an unique potential for cell therapy since they could allow the production of almost any cell type at a large scale. Accordingly, many protocols have been developed to differentiate hPSCs into cells of clinical interest, including hepatocytes. …
-
Production of hepatocytes from human pluripotent stem cells for cell-based therapy
Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy …
-
Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… with non-parenchymal cholangiocytes, endothelial cells, Kupffer cells, and hepatic stellate cells. Disorders affecting hepatocytes are life threatening and increasing in prevalence12, with end-stage treatment relying upon a limited supply of liver donations for transplant. Consequently, the …
-
CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
Human pluripotent stem cells (hPSCs) exist in multiple states of pluripotency, broadly categorised as naïve and primed states. These provide an important model to investigate the earliest stages of human embryonic development. Naïve cells can be obtained through primed-to-naïve reprogramming; …
-
Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) are an invaluable model for cellular and developmental biology, and hold great potential for translational applications. While great progress has been made in elucidating the signalling pathways regulating pluripotency and differentiation, our mechanistic …
-
DISSECTION OF HOST-VIRUS INTERACTIONS IN THE HUMAN CENTRAL NERVOUS SYSTEM
… to their ability to invade the central nervous system (CNS), and the most common cause is HSV-1. Inborn errors of immunity (IEIs) have demonstrated that neuron, cell type-specific, antiviral immunity is essential to prevent viral infection and encephalitis. However, despite these key advances in …
-
Investigating the role of cell cycle regulators in mesoderm specification
… germ layers from which the cardiovascular system, muscle and bone originate and derivatives of the mesoderm lineage are affected in a number of pathologies. Therefore, understanding the mechanisms regulating formation of mesoderm is interesting for a diversity of diseases and clinical …
-
Investigating the role of DPPA2 and DPPA4 in the Epigenetic Control of Lineage Programs in Human Embryonic Stem Cells
… co-ordination of cell fate specification during human early development is a vital yet poorly understood process. To navigate the dynamic transcriptional and epigenetic changes associated with germ layer allocation, pluripotent cells maintain developmentally important genes and their regulatory …
-
An in vitro model of human yolk sac and epiblast crosstalk
… embryo culture techniques have recently allowed human embryos to be cultured in vitro past the point at which they would implant in vivo, until the internationally accepted culture limit of 14 days. Nonetheless, the reliance on surplus in vitro fertilised (IVF) embryos, along with the ethical and …
-
A Human Pluripotent Stem Cell-Derived Model for the Investigation of Thermogenic Adipocyte Development and Function
… In the quest to increase understanding of human BAT, in my lab we have developed a novel protocol for the in vitro differentiation of human pluripotent stem cells (hPSCs) to brown adipocytes (BAs). This thesis describes how this protocol can be employed to investigate candidate genes with …
-
A Reductionist Approach to Modeling Human Corticogenesis
… due to the genetic tractability of this model system. However, as primate studies have shown, the primate brain is unique in terms of its progenitor and neuronal composition, cortical areas, scale, and gene expression. Limitations in the availability of non‐human primate and human fetal material, …