Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"human pluripotent stem cell"”.
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
Human pluripotent stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic …
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Roles for cell surface glycans in guiding human pluripotent stem cell fate
Cell-surface polysaccharides are a fundamental component of the stem cell microenvironment. They are known to modulate developmental signals- critical for pluripotency and differentiation. Nevertheless, the architecture of the cellular glycocalyx and how these structures direct the fate of human …
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Approaches to Reduce Selection of Genomic Variants in Human Pluripotent Stem Cell Culture
… conditions that reduce genomic instability in human pluripotent stem cells (hPSCs) is an unmet challenge in the field. Results from our lab and numerous research groups demonstrate that hPSCs are prone to genomic aberrations and single-cell passaging increases the rate of genomic alterations. …
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The Maturation of Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells and Their Degeneration in Glaucoma
… leading to the degeneration of retinal ganglion cells (RGCs) and eventual blindness. A need exists to better understand the maturation of human RGCs as well as their degeneration, with the goal of developing new therapeutics diseases like glaucoma. Human pluripotent stem cells (hPSCs) provide an …
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A Human Pluripotent Stem Cell-Derived Model for the Investigation of Thermogenic Adipocyte Development and Function
… In the quest to increase understanding of human BAT, in my lab we have developed a novel protocol for the in vitro differentiation of human pluripotent stem cells (hPSCs) to brown adipocytes (BAs). This thesis describes how this protocol can be employed to investigate candidate genes with …
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Characterization and application of human pluripotent stem cell-derived neurons to evaluate the risk of developmental neurotoxicity with antiepileptic drugs in vitro
… risks of damage to the developing nervous system of many chemicals are not known because these studies often require costly and time-consuming multi-generational animal experiments. Pluripotent stem cell-based systems can facilitate developmental neurotoxicity studies because disturbances in …
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Characterizing the Immunogenicity of Islet-like Cells derived from HLA-edited Human Pluripotent Stem Cells
… much hope for the therapeutic use of islet-like cells generated from human pluripotent stem cells, important challenges limit the clinical translation of this technology. In this thesis, I describe my efforts to address two of these challenges: the inability to control the composition of the …
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LONG NON-CODING RNA VENTHEART IS NECESSARY FOR VENTRICULAR CARDIOMYOCYTE SPECIFICATION AND FUNCTION
… which influence gene expression and cardiac cell fate. This thesis focuses on verifying and characterizing of one key lncRNA, VENTHEART (VHRT), which was identified as one of the top upregulated markers in cardiomyocyte (CM) development. Utilizing human pluripotent stem cell-derived …
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Characterization of the Effects of bFGF and GSK3β Inhibitors on Embryoid Bodies Derived from Human Pluripotent Stem Cells
… (EB) formation is a common first step in many human pluripotent stem cell (hPSC) differentiation protocols. Previous work suggests that EBs are sensitive to growth factor withdrawal if they are derived from hPSCs maintained in feeder independent media such as mTeSR1. To promote cell survival, …
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Regulation of amnion induction during the human formative pluripotency transition
Human pluripotent stem cell(hPSC) represents a powerful tool to model early lineage decisions due to their capacity to differentiate to all somatic lineages and primordial germ cells. hPSC exists in distinct phases—naïve, formative, and primed—each with differing developmental potential. Naïve hPSC …
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Delivery of hiPSC Derived Cardiomyocytes via an Injectable Nanofibrous Gelatin Polyurethane Composite Scaffold for Heart Tissue Repair
Cardiac cell therapy, utilizing contractile human pluripotent stem cell-derived cardiomyocytes, including embryonic stem cell-derived (hESC-CM) and induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), has shown promising results in treating ischemic cardiomyopathy and heart failure. …
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Extracellular matrix based substrates for propagation of human pluripotent stem cells
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal …
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A chemical biology study of human embryonic stem cell pluripotency and differentiation
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerative medicine. To fully realize its potentials, we not only need to further our understandings toward the molecular mechanisms governing pluripotency and differentiation, but also to find new tools to …
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… to produce antibodies is restricted to the B cell lineage and is tightly regulated, starting with the temporal separation of the recombination process, in which DH-JH precedes VH-DHJH recombination. Successful recombination of both heavy and light chain loci results in the expression of an …
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Genesis of the human germline
… this thesis was to examine the specification of human primordial germ cells (PGCs) and the mechanism regulating the transient gain and loss of competence for germ cell fate. Specification of PGCs in humans occurs during weeks 2 to 3 in gastrulating embryos, which eventually develop into sperm and …
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FROM A PLACODE PROGENITOR PLATFORM TO A TRIGEMINAL GANGLION ORGANOID: MODELLING SENSORY DEVELOPMENT AND FUNCTION IN VITRO
… rise to most of the facial peripheral nervous system. The intermediate region of the pre-placodal ectoderm later acquires a trigeminal fate, marked by the expression of PAX3, and thus develops into the TG. The TG is involved in several human pathologies, such as migraine and viral diseases, …