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Showing 1 to 6 of 6 for “"human monoclonal antibody"”.
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Production and Characterization of an Human Monoclonal Antibody IgG to TRAIL Receptor 2
… 효과적이며 안정적인 치료용 항체로 만들기 위해 Fab 를 IgG 형태로 바꾸었다. 이는 antibody engineering을 통하여 DR5-18 IgG construct를 제작하는 방법으로 수행되었으며, 포유 세포에 넣어 항체 발현을 할 수 있도록 만들어졌다. 본 학위논문 연구에서는 발현된 DR5-18 IgG를 배양액으로부터 AKTA prime plus 와 HitrapTM IMAC column을 이용하여 정제하였으며 western blotting을 통해 확인하였다. MTT assay, Annexin V-FITC/PI …
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Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… sTRAIL is able to induce apoptosis of normal human hepatocyte, and some cancer cells are resistant to sTRAIL treatment. Recently, agonistic antibodies to TRAILR1(DR4) or TRAIL-R2(DR5) have been developed for alternative anti-cancer drug. Although they specifically bound their target receptors …
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Characterization of Variable Region Structures and Antigen-Binding Properties of OxLDL-Specific Human Monoclonal Antibody Isolated from PBMC and Atherotic Plaques of Atherosclerosis Patients
… phage display, we have also cloned anti-oxLDL monoclonal antibodies from atherosclertic plaques. The VH and VL sequences of the Fab produced from the plaques were compared with those from PBMC. The total RNA were isolated from atherotic plaques. PCR was then used to amplify cDNA synthesized …
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Genome-wide CRISPR Screens to Uncover Novel Therapeutic Targets in Wnt-β-catenin Driven Gastrointestinal Cancers
… to identify novel genetic vulnerabilities in human cancer cells. Genome-wide CRISPR screens were completed in RNF43 mutant pancreatic ductal adenocarcinoma (PDAC) cells lines, which rely on Wnt-β- catenin autocrine signaling for their proliferation. These screens revealed a single Frizzled …
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Anti-Angiogenic Therapy in Non-Small Cell Lung Cancer: Characterizing a New Therapy and Investigating Potential Mechanisms of Resistance
… Bevacizumab (Avastin®, Genentech/Roche), a humanized monoclonal antibody that recognizes human VEGF and blocks VEGF from binding to VEGF receptor (VEGFR) 1 and 2, was the first anti-angiogenic drug approved by the United States Food and Drug Administration for the treatment of cancer and …
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Studies on the Function of Human Anti-HSP60 Monoclonal Antibody Fab
"목적 : 열충격 단백질(Heat Shock Protein; HSP)은 원핵 세포과 진핵 세포에서 발현된다. 열충격 단백질은 단백질이 합성될 때 올바른 접힘을 돕는 chaperone의 역할을 하며 분자량에 따라 나뉠 수 있다. 그 중에서 열충격 단백질-60은 열과 같은 충격에 의하여 미토콘드리아 내에서 발현 양이 증가되고, 세포 표면에서도 발현될 수 있다. 열충격 단백질-60은 다양한 미생물질에 의한 선천적 면역 체계 활성체 및 염증 반응 자극제로서 작용한다고 알려졌다. 인간 재조합 열충격 단백질-60은 면역 세포에서 염증 반응을 …