Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"human mammary epithelial cells"”.
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Effects of DDT and DDE on gap junctional intercellular communication in human mammary epithelial cells
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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HUMAN CLCA2 IS A P53-DEPENDENT ZINC METALLOPROTEASE AND ITS INTERACTION WITH EVA1 MAINTAINS DIFFERENTIATION OF HUMAN MAMMARY EPITHELIAL CELLS
… or purified protein from CLCA2-transfected cells, we show here that CLCA2 cleavage is catalyzed by zinc and inhibited by metal chelator EDTA. Moreover, an E165Q mutation in the metal binding site abolished processing without affecting stability or trafficking. The mutant could be cleaved by …
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Differences in the regulation of Thrombospondin-1 expression between epithelial cells and fibroblasts
… proteins. It has been demonstrated that in human epithelial cells repression of the angiogenesis inhibitor Thrombospondin-1 (Tsp-1) requires stimulation of Myc in combination with hyper-physiologic levels of oncogenic Ras. This work demonstrates that in human mammary epithelial cells, …
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The Effects of 1,25-Dihydroxyvitamin D3, Docosahexaenoic Acid and 5-Fluorouracil on Human Breast Cancer Cells
… have antiproliferative effects on a variety of human cancers, including breast cancer. Studies have shown that a combination approach to cancer treatment is more effective than any one treatment administered alone. In this study, human mammary epithelial cells from the MCF-7 cell line were …
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Quantitative studies of EGFR autocrine induced cell signaling and migration
… signaling plays an important role in normal epithelial cell proliferation, survival, adhesion and migration. Aberrant expression of the EGF receptor and its cognate ligands have been implicated in various types of cancers, hence EGF receptor autocrine activation is thought to also be involved …
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Evaluation of Chronic RalGTPase Activation as a Core Specifier of Oncogenic Transformation
… required for survival in Ras-dependent tumor cells rather than normal cells, while RalA is required for anchorage-independent proliferation but dispensable for survival. However, the spectrum of cancer cell lineages dependent upon Ral functions for tumor formation is currently unknown. We …
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14-3-3 Zeta Overexpression Serves As A Novel Molecular Switch Turning Tgf-Beta From Tumor Suppressor to Tumor Promoter
… cell proliferation of normal and premalignant epithelial cell. However, in the last stage of tumor progression, TGF-β functions as tumor promoter to enhance tumor cells metastatic dissemination and expands metastatic colonies. Currently, the mechanism of how TGF-β switches its role from tumor …
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Synthetic Lethality of Cdk Inhibition and Doxorubicin In Triple-Negative Breast Cancer Requires P53 Inactivation
… Therefore, we <strong>hypothesized that TNBC cells are sensitive to cell cycle targeted combination therapy, which leaves non-transformed cells unharmed</strong>. Our findings demonstrate that sequential administration of the pan-CDK inhibitor roscovitine prior to doxorubicin treatment is …
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Characterization of The Growth Factor Receptor Network Oncogenes in Lung Cancer
… of six GFRN oncogenes overexpressed in human mammary epithelial cells (HMECs) were generated to interrogate this pathway’s downstream crosstalk, beyond initial mutation status. Utilization of this method may reveal novel phenotypic patterns that could be used to improve targeted …
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Trim28 Interacts With Ezh2 to Regulate Its Activity
… EZH2 and regulates its activity in breast cancer cells. EZH2, the major H3K27 tri-methyltransferase, is over expressed in multiple subtypes of breast cancer; however, whether EZH2 plays an active role in breast cancer progression or treatment resistance is unknown. Here, we determined …
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Quantitative analysis of 2D and 3D models for epidermal growth factor receptor-dependent cell migration in the context of the extracellular microenvironment
… individual invasive carcinomas arise upon epithelial-mesenchymal transition (EMT) and migrate through a complex tumor microenvironment to successfully metastasize. While the activation of EGFR enhances invasiveness in vivo, it is still unclear which downstream molecular changes caused by …
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Biophysical regulation of cell motility by adhesion ligands and growth factors : effect of spatial presentation of the ligand
… presentation of EGF in an autocrine manner in human mammary epithelial cells, where the cell simultaneously synthesizes the receptor and the ligand, results in the regulation of the directionality of cell motion. Formation of cell surface EGF/EGFR complexes in an autocrine manner causes an …
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The Role of Tumor Suppressor Dear1 In The Acquisition of Mammary Stem/Progenitor Cell Properties
… from the primary tumor is driven by the epithelial-mesenchymal transition (EMT) program. <em>DEAR1</em> is a tumor suppressor gene which is mutated, undergoes loss of heterozygosity in breast cancer, and is downregulated in DCIS lesions and IDC. DEAR1 regulates acinar morphogenesis and …
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Imbalance Between Neutrophil Elastase and Elafin Promotes Breast Cancer Growth and Progression
… inhibitor, is a critical component of the epithelial barrier against neutrophil elastase (NE) activity. The central hypothesis examined in this dissertation was that elafin has tumor suppressive properties in breast cancer. In support of this hypothesis, immunohistochemical (IHC) analysis …
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The Role of Tyrosine Phosphorylation In The Functions of The Tumor Suppressor Gprc5A
… its expression is suppressed in the majority of human non-small cell lung cancer cell lines and tissues. This differential expression has led to the idea that GPRC5A is a potential tumor suppressor. This notion was supported by the finding that mice with a deletion of the Gprc5a gene develop …
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Low Molecular Weight Cyclin E Deregulates Dna Replication and Damage Repair to Promote Genomic Instability In Breast Cancer
… form of LMW-E is detected in breast cancer cells and tumor tissues, but not in normal mammary epithelial cells or adjacent normal tissues. Unlike FL-cycE, LMW-E drives mammary epithelial cell transformation in human cells and spontaneous mammary tumor formation in transgenic mouse models, …
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Trim24 As An Oncogene In The Mammary Gland
… transcriptional co-regulator, TRIM24, in mammary gland development and disease.</p> <p>We previously reported an unknown binding partner and negative regulator of p53, Tripartite Motif protein 24 (TRIM24). TRIM24 is a multifunctional protein, which acts as a co-regulator of estrogen …
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Trim24 Orchestrates Metabolic Reprogramming and Emt In Breast Cancer
… coupled with metabolic reprogramming and epithelial mesenchymal transition (EMT) in breast cancer. TRIM24 was first established by our laboratory as a previously unknown negative regulator of p53 via its RING domain, as a co-regulator of nuclear receptors and a PHD/Bromodomain reader of …
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The role of PTEN as a PI(3,4)P2 lipid phosphatase in Class I phosphoinositide 3-kinase signalling
… a molecular trigger of many pathophysiologies in humans, including autoimmune disorders, respiratory diseases and cancer. To date, two classes of lipid phosphatases SHIP1/2 and PTEN have been reported, which dephosphorylate PI(3,4,5)P3 on positions 5’ and 3’ of the inositol ring to generate …
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Establishment of the Physical and Technical Prerequisites for the Determination of the Relative Biological Effectiveness of Low-energy Monochromatic X-rays
… optimised for two established cell lines. The human mammary epithelial cells MCF-12A have been chosen due to the importance of RBE of soft X-rays for determination of risk from mammography. On the other hand, the use of the widespread mouse fibroblast cell line NIH/3T3 allows to compare the …