Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"human induced pluripotent stem cells"”.
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Derivation of lymphocytes from human induced pluripotent stem cells
Human pluripotent stem cells have the potential to produce essentially unlimited numbers of mature and functional blood lineage populations to study human hematopoiesis. Particularly, human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable …
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Using human induced pluripotent stem cells to model cystinosis
… accumulation of cystine within lysosomes in most cells of the body, however the kidney is the main organ affected. For poorly understood reasons, renal proximal tubule (PT) cells lose their functionality and degenerate in cystinosis, forming a characteristic “swanneck” lesion that leads to kidney …
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Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… partly due to a lack of accurate models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… the use of robust and tractable 3D multicellular human liver organoids (HLOs) to model drug induced liver injury (DILI) by exposing HLOs to Antiretroviral therapy drugs (ART), combination of ART and antituberculosis drugs (A+TB) and Schistosoma mansoni egg soluble antigen (SEA) or Troglitazone …
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Modeling the Effects of Mechanical Loading on Hypertrophic Cardiomyopathy Pathogenesis in Human Induced Pluripotent Stem Cell derived Micro Heart Muscle
… Promising approaches in tissue engineering and stem cell biology have significantly improved the structural and functional maturity of the engineered heart tissue derived from human induced pluripotent stem cells, which can be served as a complementary method to traditional mouse models. …
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RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology
… core genetically encoded in the genome of human induced pluripotent stem cells (hiPSCs). This architecture can be theoretically programmed to precisely control stem cell differentiation. First, we use computational biology to analyze differential miRNA expression during human stem cell …
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In vivo and in vitro approaches to characterising axon growth and metabolic alterations in Parkinson’s disease
… the time of disease onset, midbrain dopaminergic cells lose their synaptic connectivity before dying. The preposition that dopaminergic axons degenerate initially and predominantly in PD offers new critical opportunities for the development of neuroprotective and restorative therapies. However, we …
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Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
<p>The differentiation of human induced pluripotent stem cells (hiPSCs) to prescribed cell fates enables the engineering of patient-specific tissue types, such as hyaline cartilage, for applications in regenerative medicine, disease modeling, and drug screening. In many cases, however, these …
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Brain microvascular endothelial cell dysfunction in schizophrenia: a preliminary report
… (SZ). Brain microvascular endothelial cells (BMECs) have paracellular and transcellular proteins, transporters, as well as important extracellular matrix proteins, which collectively contribute to maintaining proper BBB function. While previous studies have provided some insights into …
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Engineering novel neuronal circuits using HI-PSCS derived neurons and microglia
… will remodel the connections within the human cortical neuronal circuit development, resulting in higher synchronicity and stable firing of action potentials for a longer period of time. The presence of micro-glia could also help the neuronal circuits and systems to reach maturation …
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hiPSC-Derived Cerebral Small Vessel Disease Models
… We generated *in-vitro* co-culture models using human induced pluripotent stem cells (hiPSCs) with COL4A1, COL4A2, and HTRA1 cSVD-related mutations to assess the shared role ECM plays in cSVD. These mutations induced tight junction abnormalities with Occludin discontinuity and Claudin-5 …
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Exploring mutational signatures in human cancers using human cell line models
… in vitro using isogenic experimental model systems. In this dissertation, I build on these pioneering efforts to demonstrate the concept of mutational signatures by engineering gene knockouts in various isogenic human cell line models (i.e. HAP-1 cells and human-induced pluripotent stem cells) …
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Elucidating the biology of G-protein coupled receptor 35
… Using macrophages derived from CRISPR edited human induced pluripotent stem cells, we found the T108M PSC/UC risk associated coding polymorphism to be hypermorphic in this regard. Furthermore, lack of GPR35 reduced intestinal epithelial cell proliferation at baseline and reduced tumour …
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Regulation of gene expression in macrophage immune response
… This is particularly relevant for immune cells, whose cellular function and behaviour can be substantially altered by external cues. Furthermore, understanding mechanisms behind eQTLs is hindered by the difficulty of identifying causal variants. We differentiated macrophages from induced …
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Cellular And Mechano-Active Material Approaches To Improve Disc Repair After Herniation
… disc function. Furthermore, adult disc cells are sparse and have decreased biosynthetic abilities. Disc herniations, resulting from annular tears, enable the extrusion of NP tissue which can result in spinal nerve compression and consequent pain. The gold standard for the surgical …
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MULTISCALE TECHNOLOGIES FOR ENGINEERING AND CRYOPRESERVING OVARIAN TISSUES AND HUMAN IPSCs
… for those who wish to delay childbearing. Human induced pluripotent stem cells (hiPSCs) hold tremendous potential for tissue engineering and cell-based medicine, as they have the capacity of differentiating into ectodermal, mesodermal, endodermal, and germ cell lineages. In recent years, …
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Establishment of a Novel Patient hiPSC-derived in vitro Blood-Brain Barrier Model of Collagen IV Small Vessel Disease
… the pathomolecular mechanisms leading to SVD, human induced pluripotent stem cells (hiPSC) generated from patients with mutations in COL4A1/2 genes (COL4A1G755R and COL4A2G702D), isogenic controls and multiple wild-type (WT) control lines were used to establish a novel in vitro BBB model of …
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Using molecular QTLs to identify cell types and causal variants for complex traits
… associations have been discovered for many human complex traits, and yet for most associated loci the causal variants and molecular mechanisms remain unknown. Studies mapping quantitative trait loci (QTLs) for molecular phenotypes, such as gene expression, RNA splicing, and chromatin …
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… demands and short shelf life. Our lab uses human induced pluripotent stem cells (iPSCs), as an attractive alternative supply, as iPSCs can be cultured indefinitely and differentiate into almost any cell type. Using a technique called forward programming, we over express three key …
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