Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 67 for “"human embryonic stem cells"”.
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Human embryonic stem cells for genotoxicity testing
… mammalian cell lines and primary explanted cells commonly used in vitro to analyze the genotoxic potential of various agents, often poorly reflect human physiology. Lack of standardization also limits use of primary explanted cells. Human embryonic stem cells (hESCs) have been validated as a …
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Double-Strand Break Repair Mechanisms in Human Embryonic Stem Cells
… from genotoxic and environmental stresses. Embryonic stem cells show promise for treatment of a variety of diseases as well as for providing normal human tissue to conduct scientific research. A major obstacle for their application is that genomic instability arises in stem cells after …
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Stimulations and modulations of chondrogenic differentiation of human embryonic stem cells
By using human embryonic stem cells (hESCs) as the model system, this study aims to understand the full span process of chondrogenesis, and how this process could be regulated by growth factors BMP2 and/or TGFI?1 in a controlled context both in vitro and in vivo. By culturing hESCs in 2-D EB …
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Materials and biological approach to gene delivery in human embryonic stem cells
… tool used in the study and manipulation of human pluripotent stem cells for regenerative medicine purposes. However current methods of transient gene delivery are still highly inefficient. Using materials and biologically based concepts, I aim to develop new methods and protocols to enhance …
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Materials and biological approach to gene delivery in human embryonic stem cells
… is an important tool to study and manipulate human pluripotent stem cells for regenerative medicine purposes. Yet current methods of transient gene delivery are still highly inefficient. Using materials and biologically based concepts, we aim to develop new methods and protocols to enhance the …
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TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
Differentiation and cell cycle regulation in stem cell have a key function for embryonic development, organ homeostasis and tissue repair. Recent results have shown that these two mechanisms are intrinsically connected. Indeed, cell cycle machinery directly controls maintenance of pluripotency and …
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Roles of Heparan sulfate in mesendoderm differentiation of human embryonic stem cells
Human embryonic stem cells (hESCs) are remarkable for their ability to self-renew indefinitely and differentiate into any cell type in the human body. The differentiation of hESCs is regulated by intrinsic and extrinsic signals in the stem cell niche. Heparan sulfate proteoglycans (HSPGs) are found …
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Genome-Wide Profiling Unveils Criticial Functions of P53 In Human Embryonic Stem Cells
<p>Embryonic stem cells (ESCs) possess two unique characteristics: infinite self-renewal and the potential to differentiate into almost every cell type (pluripotency). Recently, global expression analyses of metastatic breast and lung cancers revealed an ESC-like expression program or signature, …
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Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells
… of cancer cell lines. In studies in which mouse embryonic stem cells (mESCs) with targeted deletion of the Cdk2ap1 gene were used, Cdk2ap1 was shown to be required for epigenetic silencing of Oct4 during differentiation. The goal of this thesis was to examine the role of CDK2AP1 in somatic cells …
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
<p>Human embryonic stem cells (hESCs) are a unique population of cells derived from a 6 day old human embryo that can be maintained indefinitely in vitro and have the ability to differentiate to all adult cell types. In addition to their potential for cell based therapies in the treatment of …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… with diabetes. Transplantations of islet cells are an alternative treatment that could help patients to become insulin independent and presumably also delay the development of complications. However, the increasing number of diabetic patients would require large amounts of transplantable …
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CHARACTERIZATION OF DNA METHYLATION PATTERNS IN HUMAN EMBRYONIC STEM CELLS AND COLORECTAL CANCER USING WHOLE GENOME BISULFITE SEQUENCING
… Despite the importance of this mechanism in human health, our existing knowledge is restricted to gene promoters and CpG islands (CGIs) which were thought to be key regulators of epigenetics. However, less than 7% of CpGs are located within the CGIs, many CpGs remain uninvestigated resulting …
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HYDROGEN PEROXIDE-INDUCED CYTOTOXICITY AND OXIDATIVE DNA DAMAGE ON HUMAN EMBRYONIC STEM CELLS AND THEIR DIFFERENTIATED FIBROBLAST-LIKE PROGENIES
Background: Human embryonic stem cells (hESCs) are pluripotent cells derived from in vitro fertilized (IVF) embryos. Because of their origin in healthy human tissues, self-renewability and differentiate capability, hESCs can potentially be adopted as a biological model for toxicity study. To date, …
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Investigating the role of DPPA2 and DPPA4 in the Epigenetic Control of Lineage Programs in Human Embryonic Stem Cells
… co-ordination of cell fate specification during human early development is a vital yet poorly understood process. To navigate the dynamic transcriptional and epigenetic changes associated with germ layer allocation, pluripotent cells maintain developmentally important genes and their regulatory …
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Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes
Embryonic stem cells are derived from mammalian blastocysts and self-renewal and maintain pluripotency, an ability to differentiate into all types of somatic and germ cells. Recent reports showed that several genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc(c-Myc) maintained a …
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Survey Attitudes Toward Stem Cell Research
… was utilized to assess opinions regarding embryonic stem cell research based on three objectives: willingness to accept stem cells, educational background, and religion. A total of 1 13 fixed-alternative questionnaires were distributed to a diverse group of respondents. Nonprobability …
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Proliferation and Potential of Neural and Retinal Stem Cells
The term “stem cell” is often broadly applied to a range of cell types that are relatively undifferentiated and have some capacity for proliferation. In this thesis, I employ a strict definition of stem cells as cells that are capable of both self-renewal and multilineage differentiation. These …
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