Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"human embryonic stem cell"”.
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Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes
Embryonic stem cells are derived from mammalian blastocysts and self-renewal and maintain pluripotency, an ability to differentiate into all types of somatic and germ cells. Recent reports showed that several genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc(c-Myc) maintained a …
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A chemical biology study of human embryonic stem cell pluripotency and differentiation
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerative medicine. To fully realize its potentials, we not only need to further our understandings toward the molecular mechanisms governing pluripotency and differentiation, but also to find new tools to …
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Encapsulation as a 3D model for human embryonic stem cell propagation and differentiation
… disease caused by the destruction of the beta cells of the pancreas. Current treatments for are whole pancreas or islet transplantation, which is limited by scarcity of cadaveric pancreata. Therefore, developing a cell replacement therapy with renewable sources of surrogate beta cells is …
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and …
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Investigating the role of the cardiac apelin receptor using human embryonic stem cell derived-cardiomyocytes.
… Despite this, there is a lack of a suitable human based in vitro system to investigate the cardiac apelin receptor. Human embryonic stem cells (hESCs) represent a powerful tool for modelling as they are amenable to genetic editing and can be induced to differentiate to any cell type. This …
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Human Embryonic stem cell-derived A9 dopaminergic neurons as a model and therapy for Parkinson's disease
… or disease slowing treatments in PD. Embryonic stem cells can reliably generate standardised populations of A9 dopaminergic neurons. For this reason, they are an ideal candidate cell source for both cell therapies and drug screening platforms for PD. A hallmark pathology of PD involves …
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IN-VITRO VASCULARIZED TISSUE EQUIVALENTS FROM HUMAN EMBRYONIC STEM CELL-DERIVED ENDOTHELIAL AND VASCULAR SMOOTH MUSCLE CELLS
… towards success in tissue engineering and cell therapy. One of the major obstacles challenging successful tissue engineering is the formation of functional vascular networks. Secondly, these applications require scalable production of homogeneous populations of vascular cells that include …
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Cellular therapy for chronic heart failure: a key role for epicardial fibronectin
… ‘remuscularization’ of the damaged heart with human embryonic stem cell (hESC)-derived cardiomyocytes. Recently, outcomes were improved by co-delivering hESC-derived epicardium. Clinically, the main challenge remains chronic heart failure. However, hESC-cardiomyocytes alone, in the chronically …
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Characterization of Genes Specifically Expressed in Embryonic and Cancer Stem Cells
ES cells are derived from the ICM of blastocyst and have ability of self-renewal, unlimited proliferation and pluripotency that differentiate into cells of all three germ layers. The stem cell genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc (c-Myc) act alone and interact with …
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Development of novel diagnostics and therapeutics for amyotrophic lateral sclerosis
… acrylate (PGSA) and 3D scaffolds for human embryonic stem cell (hESC) and neuronal encapsulation.A Gd-TTC conjugate was developed and characterized that was shown to be highly visible under MRI and preserved the functionality of the native TTC protein in vitro. Live animal MRI imaging …
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Production of hepatocytes from human pluripotent stem cells for cell-based therapy
Human pluripotent stem cells (hPSCs) can self-renew indefinitely in vitro while maintaining the capacity to differentiate into diverse cell types, including hepatocytes. Thus, hPSCs allow for large-scale production of hepatocytes necessary for cell-based therapy. Importantly, cell-based therapy …
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Investigating the role of ZEB2 in the establishment of neuroepithelial architecture and axon tract formation
… of brain size. In this work we show that in both human brain organoids and mouse embryos ZEB2 is expressed in telencephalic neuroepithelial cells (NECs) before the switch to radial glia (RGCs). By establishing a human embryonic stem cell (hESC) model of ZEB2 heterozygous loss-of-function we show …
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Microtechnologies for Cardiovascular Tissue Engineering
… of the heart to repair itself, necessitating a cell or organ based therapy to cure the inevitable descent into heart failure. Tissue engineering is uniquely poised to significantly alter this disease burden though the fabrication of cardiac and vascular tissues in vitro. However, the challenges …
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Exploring the therapeutic potential of the apelin receptor signalling axis, and angiotensin-converting enzyme 2 (ACE2) as the SARS-CoV-2 viral entry receptor, in the cardiovascular system
… and vasodilatation in the cardiovascular system. The apelin receptor provides a tractable therapeutic target for multiple cardiovascular diseases, such as pulmonary arterial hypertension, heart failure, and bleeding disorders, where the receptor also mediates antithrombotic responses. Here, …
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The Epicardium as the Conductor of Cardiovascular Cells in Cardiac Regeneration
Stem cell therapy for cardiac repair is a field of regenerative cardiovascular medicine that is emerging as a promising therapeutic alternative to heart transplantation in patients suffering from chronic heart failure. Although promising, challenges to cell therapy for cardiac repair remain, …
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INVESTIGATING THE IMMEDIATE CONSEQUENCES OF NORMAL AND MUTANT HTT LOSS IN HD-HESC THROUGH THE DTAG SYSTEM.
… of the mutant protein can be phenocopied in cells and mice deleted of the healthy gene, suggesting that a loss of function mechanism may also contributes to HD11–13. Thus the disease seems to be the result of a two-component pathological mechanism; first a loss-of-function (LOF) due to …
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Natural and Exogenous Genome Editing In Wiskott-Aldrich Syndrome Patient Cells
… (WASp), exclusively expressed in hematopoietic cells and required for proper platelet production and lymphoid cell function. Approximately 11% of patients with WAS exhibit a phenomenon called Somatic Revertant Mosaicism which is characterized by the presence of lymphocytes which naturally revert …
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The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… characterization in model organisms, the human PS and the role these pathways play in its formation and patterning remains a mystery. In this work I focused on understanding the role and control of the WNT pathway in human PS development. Due to the ethical limitations of working with …
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