Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 103 for “"human cell lines"”.
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Estimating transcription factor binding properties in human cell lines using statistical methods and genomics datasets
… This study aimed to use ChIPanalyser on 135 human transcription factors in the K562 cell line and investigate their chromatin accessibility preferences. Quantile density accessibility was used to determine how transcription factor binding changed when considering different levels of chromatin …
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Mass Spectrometric Analysis of Cytoplasmic Ribosomal Proteins in Drug Resistant and Drug Susceptible Human Cell Lines
… accompany drug resistance in MCF-7 breast cancer cells. Differences in ribosomal protein composition between drug susceptible and drug resistant cell lines were examined. Ribosomes were isolated from mitoxantrone susceptible and mitoxantrone resistant MCF-7 cells. The acid extracted ribosomal …
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Cell intrinsic innate immunity protein RPRD2 restricts transcription from the HIV-1 LTR in human cell lines
… latency after infection in resting CD4+ T-cells which prevents the host from clearing the virus. Highly active antiretroviral therapy effectively keeps HIV replication under control, however, the latent reservoir remains unaffected, thus therapy is never curative. The establishment and …
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Electrorotation-based characterization of human cell lines: a molecular and dielectrophoretic approach [Caratterizzazione di linee cellulari umane basata sull’elettrorotazione: un approccio molecolare e dielettroforetico]
… delle proprietà dielettriche delle cellule viventi, con implicazioni sia per la ricerca di base sia per applicazioni biomediche. Sviluppando e validando buffer biocompatibili, sono state stabilite condizioni sperimentali ottimali per preservare la vitalità cellulare e l’integrità …
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Investigating the anti-leukaemic effect of tamoxifen in the myeloproliferative neoplasms
… haematopoietic stem and progenitor cells (HSPCs) in preclinical mouse MPN models, which prompted a Phase-II clinical study (Effects of TAMoxifen on the Mutant Allele Burden and Disease Course in Patients with MyeloprolifeRatIve Neoplasms, TAMARIN). In the TAMARIN study, the safety …
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Ribosomal Proteins RPLP1 and RPLP2 are Host Factors Critically Required for Flavivirus Infectivity by Promoting Efficient Viral Translation Elongation.
… and efficient flavivirus infection of human cell lines and Aedes aegypti mosquitoes, which are natural vectors for these viruses. We hypothesized that RPLP1/2 are accessory ribosomal proteins that function to promote translation of specific cellular mRNAs sharing undefined features with …
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Characterization of Tumorigenic Proteins
… T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are identical. The 84K protein …
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Molecular mechanisms of cadmium toxic
… evidence about its mechanism(s) of action in the cells. The present study was therefore undertaken with the aim of defining the role of oxidative stress, intracellular Ca²⁺ alteration via phospholipase C- Inositol-1,4,5-triphosphate (PLC-IP3) and mitochondrial-cytochrome c dependent pathways in …
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Structural Studies into the Mechanism and Organisation of Mammalian Respiratory Complex I
… its constituent supercomplexes from immortalised human cell lines remains limited despite its potential as a system for studying human complex I disease mutations and its unique metabolic adaptations. Chapter 2 uses structural data from two high-resolution cryo-EM datasets (each up to 2.2 Å) of …
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Isolation and characterization of cathepsin Z, a lysosomal cysteine proteinase
… Z is a cysteine proteinase found in lysosomes of human cells. It was detected in human cultured cell lines using the peptidyl diazomethane inhibitor Fmoc-Leu-Leu- [¹²⁵]Tyr-CHN₂. The labeling of cathepsin Z by the inhibitor was both time- and concentration-dependent, and the proteinase was found in …
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Characterization of a polypeptide factor that inhibits the growth of a human breast cancer line in vitro
… inhibited proliferation of several malignant human cell lines, and, in particular, a line designated UCT-BR-1, which was derived from a human breast cancer metastasis. The work is presented in four chapters. Chapter 1 provides a review of the relevant literature at the time of writing; …
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Genomic and microscopic dissection of nuclear compartmentalization and function
The spatial genome organization in the cell nucleus is non-random and important for gene expression regulation. Abnormal nuclear organization is related to disease. Microscopy measurements of individual genes reveal a population-based, statistical gradient of increased gene activity from the …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… of biological functions with implications in human diseases. The process is catalysed by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), that add and remove the sugar moiety, respectively. Ogt gene knock-out is embryonic lethal in a range of animal models, hampering the study of …
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Targeting isoprenoid biosynthesis for drug discovery
… and infectious diseases are huge threats to human beings. With the rapid rise in drug resistance, there is a need for new drugs, and isoprenoid biosynthesis is an attractive target for drug discovery. In chapter 1, I briefly introduce the isoprenoid biosynthesis pathway and two important drug …
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Studies of the UNC-45A Molecular Chaperone Expression in Human Cancer
… encode two UNC-45 genes: the UNC-45A(a) (general cell isoform) is expressed in many organs, whereas the UNC-45B(b) (striated muscle isoform) is exclusively expressed in striated muscle tissues. Our project reported UNC-45A mRNA and protein expression elevated in human cell lines derived from …
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Studies of the UNC-45A Molecular Chaperone Expression in Human Cancer
… encode two UNC-45 genes: the UNC-45A(a) (general cell isoform) is expressed in many organs, whereas the UNC-45B(b) (striated muscle isoform) is exclusively expressed in striated muscle tissues. Our project reported UNC-45A mRNA and protein expression elevated in human cell lines derived from …
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Study and engineering of nucleic acid-binding repeat proteins
… fibrosis. Coexpression of these proteins in human cell lines allowed homologous recombination-mediated repair of the episomal and chromosomal reporter gene interrupted with the sequence fragment from CFTR. In the next project, the binding dynamics of TALE on DNA substrates was investigated …
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Understanding Genomes Through Engineered Structural Variation
Sequencing of the human genome has provided us with a detailed map of its content. While enormous progress has been made towards understanding the 1% of the human genome that is protein coding, we are still mostly in the dark about the function and relevance of the remaining 99%. Progress has been …
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Identification and characterisation of Pu.1-driven gene regulatory complexes and networks in Acute Myeloid Leukaemia.
… of both mature lymphoid and myeloid cells. The dysregulation of key haematopoietic transcription factors, including PU.1, is a cardinal feature of acute myeloid leukaemia (AML) and PU.1 is either deleted or mutated at low frequency in AML, suggesting it as a tumour suppressor. …
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