Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"housekeeping genes"”.
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Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis
… fragment length polymorphism (AFLP), analysis of housekeeping genes and 23S rRNA gene. A number of studies have reported C. concisus strains of GS2 to invade human epithelial cell line Caco2, and suggested that C. concisus strains of the two genomospecies may have different virulence potential. …
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Developmental transitions of germ cell lineage of the mouse
Mammalian germ cells arise during early embryogenesis and migrate to the developing gonad where, under the direction of the somatic environment, they initiate distinct sex-specific developmental programs resulting in the production of egg or sperm. Our understanding of the molecular mechanisms …
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Immunological markers of protective immune reconstitution in HIV infected persons sensitised by Mycobacterium tuberculosis
… persons. Transcriptomic analyses of 30 genes normalized to 3 different housekeeping genes, showed an overall increase in the expression of T cell memory specific genes, illustrating the regeneration of the memory T cell pool in HIV infected adults on ART. Larger number of central memory …
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A Functional Analysis of microRNAs in Nicotiana tabacum
… in tobacco was conducted. In this analysis, 12 housekeeping genes were tested for their effectiveness in serving as reference genes for gene expression analyses in tobacco. QRT-PCR was used to quantify the amount of expression of each candidate reference gene, and a new comprehensive reference …
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Targeting a Transcriptional Activator Induces Changes in Large-Scale Chromatin Structure and Nuclear Positioning
… rationale for the observed clustering of housekeeping genes within Mbp-sized chromosome sites.
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Phylogenetic Analysis and Characterization of Plant, Environmental, and Clinical Strains of Pantoea
… Multilocus sequence analysis (MLSA) on six housekeeping genes of Pantoea revealed that some species are mixed, and contain plant, clinical, and environmental strains, while other species groups are composed of only plant or only clinical strains. Comparative growth assays in maize, onion, …
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Strain-resolved transcriptomics: exploring functional heterogeneity of the gut microbiota in health and disease
… bacterial lineages and relates to IBD pathogenesis. Using paired metagenomics and metatranscriptomics data from 1,067 fecal samples (103 IBD and 335 non-IBD patients), we first constructed phylogenetic trees for over 250 bacterial species using the single nucleotide variants within essential …
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Diversity and virulence of the genus Cronobacter revealed by multilocus sequence typing (MLST) and comparative genomic analysis
… Cronobacter genus, by employing the alleles of 7 housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB and ppsA, total length 3036 bp). The 325 Cronobacter spp. strains used in the study included isolates from the highly publicised Cronobacter cases from USA in December 2011. The scheme …
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Gene Expression in Endometrial Tissues of Normal Mares and Mares With Delayed Uterine Clearance
… reproductive tract conformation. The etiopathogenesis of DUC has not been fully elucidated but suggested causes include poor genital conformation, a cranioventrally tilted uterus, defective myometrial contractions, decreased intrauterine immune activity, inappropriate lymphatic drainage or …
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Meccanismi fisiologico-molecolari di germinazione in specie spontanee
… dormancy and in its maintenance. Five probable housekeeping genes, useful as reference genes for quantitative PCR (qPCR) analysis in seed tissue, were identified in Chrysanthemum coronarium and in Leucanthemum vulgare, isolated and confirmed with comparative analysis with homologous genes in …
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Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens
… using QIAGEN QIAamp® DNA FFPE Tissue kit. Housekeeping genes were detected by endpoint PCR using standard primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) sequences to determine the quality and integrity of extracted DNA for downstream PCR amplification experiments. HrHPV DNA …
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The use of genetic materials, RNA, to determine the age of saliva stains
… quantity ratio (RQR) of two species of the housekeeping genes (β-actin mRNA and 18S rRNA) that were used in a previous study assessed on bloodstains. In the second pilot approach, the relationship assessment was carried out between the saliva's age and the relative quantities of two …
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Examination of the nematostella vectensis holobiont by comparative bacterial genomics and metatranscriptomics
… transcripts revealed bacterial expression of housekeeping genes such as those for replication, ribosomal structure and ATP-synthesis dominated by Proteobacteria, in particular Gammaproteobacteria. Further recruitment of the transcripts to sequenced Nematostella associates revealed an active …
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Prepartum Energy Intake and Its Relationship to Periparturient Inflammation and Metabolic Dysfunction in Dairy Cows
… the periparturient period. In experiment 1 housekeeping genes were evaluated for use with qPCR experiments in liver tissue. Ribosomal protein, S9 (RPS9) was most stable over dietary treatments and physiological states. In experiment 2, a qPCR study was conducted using 6 cows per treatment …
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Regulation and function of interphase histone H1 phosphorylation in pluripotent cell differentiation
… start sites (TSSs) of pluripotency factor genes is diminished after differentiation. Selective inhibition of CDK7 and CDK9, or siRNA depletion of CDK9 rapidly diminishes both the global levels and the enrichment of pS187-H1.4 at housekeeping genes. Moreover, inhibiting transcription with …
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Uncovering the Redox Potential of the Global Gene Regulatory Protein DksA in Borrelia burgdorferi
… is responsible for reducing the expression of housekeeping genes during the stringent response, while promoting the expression of virulence genes during infection. The mechanistic details of how DksA is able to sense environmental signals in B. burgdorferi is poorly understood. DksA harbors a …
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STAUROSPORINE-INDUCED APOPTOSIS/PROGRAMMED CELL DEATH
… (Chen et al., I 998). These are the sites of housekeeping genes in euchromatin domains. The hallmark apoptosis manifestations have generally been suggested to be mediated by caspase cascade involving specifically the CPP32/caspase-3-like proteases. However, various …
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Host and Bacterial Determinants of Staphylococcus aureus Nasal Colonization in Humans
… nasal carriage plays a critical role in the pathogenesis of staphylococcal infections and SA eradication from the nares has proven to be effective in reducing endogenous infections. To understand SA nasal colonization and its relation with consequent disease, assessment of nasal carriage dynamics …
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An evaluation of cancer biomarkers in normal ovarian epithelial cells and ovarian cancer cell lines
… to validate expression levels of the following genes: HDAC1, HDAC2, HDCA3, HDAC5, HDAC6, HDAC7, HDAC8, LPAR1, LPAR2, MUC16 and FOSL1, against normal housekeeping genes GAPDH and HPRT. In addition, immunocytochemistry was also used in the validation process of the aforementioned genes. …
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Determining role of bacteria associated with bacterial spot on pumpkin and squash fruit in the North Central Region of the United States
… were subjected to MLST analysis with three housekeeping genes (fusA, gltA, and gyrB) sequenced for each isolate. Results of MLST analysis showed no genetic variation among 20 X. cucurbitae isolates. A phylogenetic tree was generated for various Xanthomonas species using concatenated …
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