Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"homodimers"”.
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An investigation into the role of Bcl-3 in toll-like receptor signalling
… interacts with and blocks the ubiquitination of homodimers of the NF-κB subunit, p50. Through stabilisation of inhibitory p50 homodimers, Bcl-3 negatively regulates NF-κB dependent inflammatory gene transcription following TLR activation. Firstly, we investigated the nature of the interaction …
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The Novel Purification and Biophysical Characterization of CXC Chemokines
… CXC family of chemokines are active as monomers, homodimers, and heterodimers, with binding affinities for multiple CXC G-protein-coupled receptors. This intricate network has been linked to many cell signaling pathways, but the overall protein folding mechanisms as a concentration driven …
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Investigating the Interactions Between the Thiolate Ligand and Mutants of a Conserved Tryptophan in the Proximal Heme Pocket of the Oxygenase Domains of Endothelial and Staphyloccus Aureus Nitric Oxide Synthases
… electronegativity over wild-type. As the homodimers had already been investigated, saNOS was an ideal system in which to explore heterodimers. Heterodimers were generated through the co-expression of one wild-type and one mutated subunit, enabling the examination of each subunit …
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Defining the active form of ribonucleotide reductase from Saccharomyces cerevisiae in vitro and in vivo
… of the R2 subunit. As isolated, Y2 and Y4 are homodimers and neither can bind iron to generate the ... cofactor. When mixed, the Y2 and Y4 homodimers rapidly form a heterodimer that can be activated with iron to form the ... cofactor located in the Y2 subunit. While the apo-Y2Y4 protein is …
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Novel pharmacophore clustering methods for protein binding site comparison
… able to handle a larger dataset of 159 protein homodimers. For the steroid dataset, the resulting classification of ligands shows good correspondence with a classification based on binding affinity. For the antibody-antigen dataset, the classification of antigens reflected both antigen type and …
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Exploring the Structural Dynamics of Bacterial Chemotaxis
… was applied to intact, functional chemoreceptor homodimers to observe ligand-induced changes. This thesis describes work to interrogate the structural dynamics of chemoreceptor homodimers associated with ligand binding, using single-molecule FRET. Chapter 2 describes the construction and …
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Foamy Virus Enzymes - Activity, Regulation and Resistance
… of ATP. Retroviral proteases are only active as homodimers. In this work, analysis of the PR-RT of prototype foamy virus and simian foamy virus isolated from macaques by analytical ultracentrifugation and size exclusion chromatography indicate, that foamy virus proteases are stable and inactive …
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Characterization of the Role of Glutaredoxin 3 and Its Binding Partners In Maintaining Iron Homeostasis In Saccharomyces Cerevisiae
… Grx3 and its paralog Grx4 form [2Fe-2S]-bridged homodimers with the conserved Grx domain active site Cys and GSH as Fe-S cluster ligands.</p> <p> We have also co-expressed recombinant Grx3 with Fra2, another key player in the Aft1 iron regulation pathway. We have shown that co-expression of …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… extra enzymatic activities is unknown. ADAT2 homodimers from trypanosomes and humans have been shown to be stable in vitro but these homodimers show dramatically decreased tRNA binding and have no detectable enzymatic activity toward the typical tRNA substrates. While crystallographic data …
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Stress and release : chemical modulation of secondary metabolite production in Aspergillus sp.
… prenylated, and biosynthetically derived from homodimers of amino acids. The majority of the upregulated compounds were the aspulvinones, a class of butenolides. Structural elucidation of four isolated aspulvinones revealed both known and novel structures. Inducer concentration had a …
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The synthesis of WAY-derivatives to find linker location and a new linker to attach a fluorescence tag for homo- or hetero-dimer of 5-HT receptor ligands
… dimeric ligands. In addition, a new linker for homodimers of M100907 derivatives has been developed. Through this new linker, a fluorescence tag can be attached to the dimeric ligands. This will be used to both probe the activity of receptor dimers as well as visualize them.
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Regulation of E. coli SOS mutagenesis by dimeric intrinsically disordered umuD gene products
… Counterintuitively, UmuD2 and UmuD'2 form stable homodimers in vitro at concentrations where little or no a helix or 13 sheet is detectable. High protein concentrations and certain crowding agents can confer more typical secondary structure on UmuD2 and UmuD'2.
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Translational Regulation of the C-Jun Proto-Oncogene
… binds to DNA in the form of dimers. It can form homodimers with itself and heterodimers with Jun family (JunB and JunD), Fos family (FosB, Fra1 and Fra2), or with CREB family members through the leucine zipper motif. Because the <em>c-jun</em> proto-oncogene plays an important role in cell …
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Structural Investigation of the Chromatin Remodeller Fun30
… (Function Unknown Now 30) and Chd1 function as homodimers and monomers respectively and represent tractable systems for structural investigation in comparison to multi-subunit complexes. Here systems are described for expression of Fun30 and Chd1 protein fragments from <i>Saccharomyces …
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DNA Bending by Steroid Receptors
… (DBD), the human PR DBD, PR-A:A and PR-B:B homodimers, and PR-A:B heterodimers to the HRE. We find that PR-A:A, PR-B:B and PR-A:B all induce approximately the same degree of DNA bending and that the GR and PR DBDs induced DNA bending on binding to the HRE We also determined whether binding …
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Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection
… IL-12p35¯/¯ mice still produce IL-12p80 homodimers and IL-23. In infection with T.b. brucei and T.evansi; IL-12p35¯/¯, IL-12p40¯/¯ or IL-12p35¯/¯/p40¯/¯ mice were susceptible to both these pathogens, demonstrated by increased mortality compared to wild type C57BL/6 mice. The different …
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Discovery and characterization of a small molecule that modulates c-Myc mediated transcription via max homodimer stabilization
… bHLHLZ transcription factor. Max also forms homodimers as well as heterodimers with other proteins including the Mxd family of proteins, creating a dynamic network of protein-protein interactions to regulate transcriptional programs. Despite the significance of this network, the arsenal of …
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High Throughput Screening for Small Molecule Interactions with Nucleic Acid Binding Proteins
… system to favor transcriptionally repressive MAX homodimers, effectively reducing MYC transcriptional activity. Assays against the MYC target LIN28B were developed for further indirect inhibition of MYC; however, an unexpected biological interaction with cellular reporters prevents further …
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Biochemical Characterization of the DUF3328 Protein in the Biosynthesis of Cyclic Peptide Cyclochlorotine
… that DUF3328 proteins form disulfide-linked homodimers, an unusual feature among biosynthetic enzymes. To elucidate their biochemical roles, I purified CctR and CctP2 from Sf9 insect cells, overcoming challenges posed by their membrane association and intrinsic disorder. In vitro assays …
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