Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"homodimer"”.
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Chemokine CXCL7 Monomer, Homodimer, and Heterodimer: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions
… assays, the structural features of the monomer, homodimer, and heterodimer, their receptor activity, and GAG interactions have been successfully characterized. Our findings indicated that the receptor activity and binding interactions were similar for all the variants, with the CXCR2 N-domain …
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Chemokine CXCL7 Monomer, Homodimer, and Heterodimer: Structural Insights, CXCR2 Receptor Function, and Glycosaminoglycan Interactions
… assays, the structural features of the monomer, homodimer, and heterodimer, their receptor activity, and GAG interactions have been successfully characterized. Our findings indicated that the receptor activity and binding interactions were similar for all the variants, with the CXCR2 N-domain …
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Discovery and characterization of a small molecule that modulates c-Myc mediated transcription via max homodimer stabilization
… bHLHLZ transcription factor. Max also forms homodimers as well as heterodimers with other proteins including the Mxd family of proteins, creating a dynamic network of protein-protein interactions to regulate transcriptional programs. Despite the significance of this network, the arsenal of …
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DNA Recognition by Helix-Loop-Helix Proteins
… to its target DNA revealed that this symmetric homodimer folds into a novel parallel left-handed four-helix bundle, which is globular and stabilized by a well-defined hydrophobic core. Two pairs of α-helices protrude in opposite directions from the bundle. One, the basic regions, enters the …
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Structure and Function of Colicin E5 Ribonuclease Domain
… ColE5. The structure comparison of ColE5 in homodimer and complex showed that ImmE5 not only blocked but also closed the cleft of the active site of ColE5.
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Potential Energy Surfaces for Intermolecular Proton Transfers in Crystalline Aspirin: A Computational Study
… such a system was examined. First an aspirin homodimer was modeled with various basis sets and levels of theory to develop an efficient theoretical model to use in modeling the proton transfer in a larger crystal model. Both ab initio molecular orbital models and density functional theory …
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Molekulare, biochemische und strukturelle Untersuchungen an amylolytischen Enzymen von Thermotoga maritima MSB8
… konnte gezeigt werden, daß das native AglA als Homodimer aus zwei identischen 55 kDa Untereinheiten vorliegt. Dabei spielt die An- oder Abwesenheit der genannten aktivierenden Komponenten für die Dimerisierung keine Rolle. Zur Klärung der Funktion konservierter Aminosäurereste wurden …
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Computational design of orthogonal antiparallel homodimeric coiled coils
… design of six orthogonal antiparallel homodimeric coiled coils. Five of these designed sequences were experimentally tested, of which only three of the sequences adopted the target antiparallel homodimer topology. All three of these sequences, as well as a previously designed …
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Structure-function studies of the Parkinsonism-linked protein DJ-1
… we found that DJ-1 binds one Cu(I) ion per DJ-1 homodimer. The structure of Cu(I)-bound DJ-1 reveals a new biscysteinate metal binding motif formed by juxtaposed Cys-53 at the homodimer interface. We calculated a subfemtomolar dissociation constant (Kd = 6.41 x 10-16 M) for Cu(I) that supports …
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Funktion des Wind-Proteins in Drosophila melanogaster bei Faltung und/ oder Transport des sekretorischen Proteins Pipe
… von 1,9 A kristallisiert werden. Es liegt als Homodimer vor- bestehend aus einer N-terminalen Thioredoxin- und C-terminalen D-Domäne. Beide Domänen werden über einen flexiblen Linker verbunden.Über Punktmutationen an Wind konnte eine Bindestelle für Pipe identifiziert werden. Dabei handelt es …
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Structural and Functional Studies of the Munc13 MUN Domain and the RIM C2B Domain
… revealed the existence of a RIM1 C2B domain homodimer, which was confirmed by analytical ultracentrifugation and NMR spectroscopy. Our results suggested a model that RIM1 C2B dimerization might facilitate the Munc13 C2A homodimer to Munc13 C2A/RIM zinc finger heterodimer switch during …
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DISCOVERY AND BIOPHYSICAL CHARACTERIZATION OF ALLOSTERIC INHIBITORS OF FACTOR XIa (FXIa)
… of the coagulation cascade. FXIa, a 160 kDa homodimer is gaining popularity of late as a potential target for anti-thrombotic agents due to its relative safety. A number of inhibitors which target the active site of FXIa have been reported but to our knowledge there have been no inhibitors …
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Nanoparticle-nanoparticle interactions and light-matter interaction studied by simulated absorption spectroscopy
… calculations, the absorption spectra of homodimer and heterodimer nanoparticles are explored. Then, plasmon-induced excitation energy transfer is simulated with an attosecond time step up to investigate processes occurring on the picosecond dynamics time scale. Third, the naphthalene …
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Biophysical Characterization of the ALS-Linked Protein TDP-43; and Genetic and Small Molecule Rescues from Its Cytotoxicity
… and <em>in vitro</em>, to form a native homodimer, but the precise interface of dimerization is unknown. The mechanism of aggregation is also unknown. As ALS is a disease currently without cure, anti-aggregation and dis-aggregase therapeutics are needed. This thesis sought to probe the …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
… of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the maturation of the 16S and 23S rRNAs, the degradation of mRNA, and the …
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Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection
… IL-12p35¯/¯ mice still produce IL-12p80 homodimers and IL-23. In infection with T.b. brucei and T.evansi; IL-12p35¯/¯, IL-12p40¯/¯ or IL-12p35¯/¯/p40¯/¯ mice were susceptible to both these pathogens, demonstrated by increased mortality compared to wild type C57BL/6 mice. The different …
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Activation of acetate in acetate-grown Methanosarcina thermophila: purificationm and characterization of acetate kinase
… The native enzyme (Mr 94,000) was an α₂ homodimer with a subunit Mr of 53,000. Activity was optimal between pH 7.0 and 7.4 and was stable to heating at 70°C for 15 min. The apparent Km for acetate was 22 mM (Vmax = 668 μmoles ADP/min/mg protein) and 2.8 mM for ATP (Vmax = 777 pmoles …
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Evidence that ARNT plays a role in the regulation of the immunoglobulin heavy chain enhancer and identification of a putative ARNT ligand
… that increases DNA-binding activity of the ARNT homodimer. This ligand was partially characterized by UV crosslinking studies and a variety of biochemical studies using electrophoretic mobility-shift assays. Preliminary data suggests that it is hydrophilic, heat-stable, small, and non-protein.</p>
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ΦΩΣΦΟΡΥΛΙΩΣΗ ΠΡΩΤΕΙΝΩΝ ΣΕ ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙΠΟΝΤΙΚΟΥ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΜΙΑΣ ΝΕΑΣ ΚΙΝΑΣΗΣ ΠΡΩΤΕΙΝΩΝ ΠΟΥ ΡΥΘΜΙΖΕΤΑΙ ΑΠΟ ΚΥΚΛΙΚΑ ΝΟΥΚΛΕΟΤΙΔΙΑ
… IN ALL PROBABILITY THE NATIVE ENZYME IS A HOMODIMER. IT ACTS OPTIMALLYAT PH 7.5 AND PHOSPHORYLATES HISTONES AND POLYSOMAL PROTEINS BUT NOT PROTAMINE, CASEIN OR ANY OF THE PROTEINS OF THE "PH5" FRACTION. AT HIGH (10- 5 M) ATP CONCENTRATIONS MG2+ IS THE MOST EFFICIENT ACTIVATING METAL WHILE …
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Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
… domain protein that creates a homodimer with another MRAP1 protein. Furthermore, MRAP2 creates a heterodimer with the MC2R. Previous studies have shown that the MRAP1 protein contains an activation motif required for activation of MC2R and this activation motif located on the …
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