Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"holin"”.
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… LCT has been shown to be dependent on a cognate holin located in their pathogenicity loci. The holins for C. difficile and P. sordellii share similar membrane topologies to that of the phage lambda holin. However, TpeE in C. perfringens shares a similar membrane topology to TatA, a key protein in …
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Characterization of the Bacteriophage Felix O1 Endolysin and Potential Application for Salmonella Bioremediation
… is to use bacteriophages, or the purified holin and endolysin proteins that make them lethal and lytic, as antimicrobial food treatments or additives. The bacteriophage Felix O1 is a promising candidate for development as an anti-Salmonella food treatment. This dissertation describes the …
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Recombinant collagen production optimization in Escherichia coli
… lytic gene (bacteriophage T4 t-holin) along with the basally expressed T7 lysozyme gene from the pLysS plasmid. A methodology for the optimization of the sequential induction of CLP3.1-his followed by induction of the t-holin is presented; a review of the literature suggests that …
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Investigating the key components of the chitinase secretion system in <i>Serratia marcescens</i>
… and <i>chiX </i>genes encode putative holin and endolysin proteins, respectively, and these are essential for secretion of chitinases. Using X-ray crystallography, the structure of full-length ChiX was resolved to 1.34 Å, revealing structural similarities to the lysostaphin-type (LAS) …
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Secretion of the chitinolytic machinery in Serratia marcescens
… A genetic screen identified genes encoding a holin-like membrane protein (ChiW) and a putative L-alanyl-D-glutamate endopeptidase (ChiX). Subsequent quantitative proteomics experiments and biochemical analyses established that ChiW and ChiX were required for secretion of the entire …
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Secretion and regulation of the chitinolytic machinery in <i>Serratia marcescens</i>
… identified two genes (chiW and chiX) encoding a holin-like protein and a putative endopeptidase, that are essential for secretion of the chitinolytic machinery. Moreover chiA was found expressed by a small subpopulation of cells and in coordination with chiX. This project used fluorescence …
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Identifizierung immunodominanter antigener Strukturen von Methicillin-resistenten Staphylococcus aureus (MRSA) mit Hilfe einer Expressionsgenbank
… immunodominanten Antigenstrukturen umfassen ein Holin, die Amidase LytA, Faktoren des isd-Genclusters, der für die Cadmiumresistenz wichtige Transporter CadA, unbekannte Proteine der Staphylokokken-Pathogenitätsinsel SaPI3 und Protein A. Interessanterweise wurden durch den methodischen Ansatz …