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Showing 1 to 6 of 6 for “"hnRNP L"”.
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Context-Dependent Function of the Splicing Factor hnRNP L
… this mode of regulation, is the human CD45 gene. HnRNP L binds to a motif present in both CD45 variable exons 4 and 5 to affect their coordinate repression. Previously, it was shown that hnRNP L regulates exon 4 by stalling the U1 and U2 snRNPs in a non-permissive A-like exon-defined spliceosomal …
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Combinatorial Regulation of Signal-Induced CD45 Exon Repression by hnRNP L and PSF
… silencing under resting conditions by binding to hnRNP L. ESS1 is also sufficient to confer the activation-induced increase in exon repression, and this document describes two mechanisms responsible for mediating this effect. First, hnRNP L silencing function is slightly increased in activated …
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Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… sequence (ESS1) within CD45 exon 4 is bound by hnRNP L to induce its skipping. HnRNP L represses spliceosome assembly at a step after the binding of U1 and U2 snRNP on either side of the exon. My goal is to understand how hnRNP L perturbs U1 snRNP binding at the 5' splice site (5' ss) to cause …
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The Regulation of the Alternative Splicing of Caspase 9
… and mass spectrometry analysis identified hnRNP L as part of these protein:RNA complexes. Downregulation of hnRNP L induced a significant increase in caspase 9a/caspase 9b mRNA ratio, which translated to the protein level. Expression of hnRNP L verified the siRNA specificity lowering the …
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Formation of an Exon-Defined A Complex Spliceosome Intermediate Results in CD45 Exon Repression
… the CD45 gene. ESS1 is bound by at least three hnRNP proteins (hnRNP L, PTB, hnRNP E2) of which hnRNP L is the main functional component. Subsequent work showed that ESS1 does not regulate CD45 exon 4 splicing by the typical mechanism of preventing exon definition. Instead, spliceosome assembly …
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Characterization and Development of Strategies for Altering Protein Expression in JSL1 Cells
… assays have shown that the regulatory protein hnRNP L binds to this silencer sequence and results in basal exon repression during resting conditions and undergoes modifications which further influence exon skipping upon stimulation. Furthermore, in vitro assays indicate that upon stimulation, …