Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"histone variants"”.
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An electrophoretic method for the isolation of isohistones from the embryo of the sea urchin Parechinus Angulosus
… aim of this project to isolate sea urchin embryo histone variants by preparative polyacrylamide gel electrophoresis and to prove me identity of the isolated proteins by amino acid composition and partial sequencing. This serves two purposes, namely, the unequivocal identification of chromosomal …
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The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme
… This project is a study of the very lysine-rich histone fraction or H1-histone fraction of Parechinus angulosus embryo. The first part deals with the characterization and separation of the H1-variants present in the late gastrula embryo. The second part describes the determination of the partial …
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Regulation of Gene Expression in Trypanosoma brucei
… the transcription machinery. T. brucei has four histone variants: H2AZ, H2BV, H3V and H4V. Using ChIP-seq to examine the genome-wide distribution of chromatin components, I showed that H2AZ, H2BV, the K10-Ac form of H4, and the bromodomain factor BDF3 are significantly enriched at probable RNA …
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The role of linker histone variant in double strand break repair
… to access the DNA. It has been shown that linker histone variants inhibit the double strand break (DSB) repair pathway known as non-homologous end joining (NHEJ). NHEJ, in vivo, requires the KU complex, the DNA dependent protein kinase (DNA-PK) and the DNA ligase IV/XRCC4 (LX) complex. In vitro …
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Regulation and function of histone variant H2BE in neurons
… genome that organizes DNA and is made of four histone proteins: H2A, H2B, H3, and H4. Regulation of histone proteins affects gene expression through multiple mechanisms, including exchange with histone variants. While variants of H2A and H3 have well defined roles in chromatin regulation, …
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Deoxyribonuclease probing on sea urchin embryo chromatin
… urchin, Parechinus angulosus, embryo and sperm histone variants play in chromatin structure has been investigated. Chromatin structure has been determined at different levels of resolution in sperm and in developing embryos using micrococcal nuclease, pancreatic deoxyribonuclease (DNase I) and …
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Roles of H2A.z in Fission Yeast Chromatin
Covalent histone modifications such as methylation, acetylation as well as differential incorporation of histone variants are shown to coincide with different chromatin compartments and mark active or repressed genes. Msc1 is one of the seven JmjC Domain Proteins (JDPs) in Fission Yeast. JDPs are …
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Roles of the Histone Chaperone FACT in Drosophila Gene Regulation and Chromatin Architecture
The highly conserved histone chaperone FACT (Facilitates Chromatin Transcription) is thought to contribute to the disassembly and reassembly of nucleosomes in the wake of RNA polymerase II passage through chromatin. In yeast, mutation of FACT subunits leads to decreased nucleosome occupancy at the …
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Structural and functional effects of histone variant, H2A.Bbd, on the nucleosome core particle
… pair (bp) of DNA wrapped in 1.65 turns around a histone octamer containing two copies each of the four core histone proteins (H2A, H2B, H3 and H4). Chromatin creates an impediment to the processes of DNA transcription, replication, repair and recombination. In addition to histone covalent …
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The role of heterochromatin markers in lytic human herpesvirus egress and maturation
… 147bp of DNA wrapped around an octamer of histone proteins. The structural and transcriptional control of the chromatin is tightly regulated and closely linked. Transcriptionally active, open chromatin is referred to as euchromatin, while tightly packed, transcriptionally repressed …
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Control of Regulatory Element Function by Histone H3.3
In eukaryotic cells, DNA is wrapped around histone proteins to form nucleosomes, the fundamental repeating unit of chromatin. While chromatin functions in part to organize a large amount of genomic material within the confines of the nucleus, regulatory DNA sequences consequently become masked to …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
<p>Histones are architectural proteins that wrap approximately two turns of DNA around their octameric core structure, constituting the fundamental packaging unit of eukaryotic chromatin — the nucleosome. Beyond their structural role, they regulate virtually all processes that act on or depend on …