Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"histone gene"”.
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Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
This thesis investigates whether DNA and histones contain sufficient information to direct nucleosome cores into specific positions. The "in vitro" assembly of nucleosome cores promoted by poly(glutamic acid) has been optimized with respect to rate and yield. This was achieved by paying attention …
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Physical mapping of an early sea urchin gene battery from Parenchinus angulosus
… aim of this project was to characterise an early histone gene battery isolated from Parenchinus angulosus. An early histone gene battery (named H27) which was believed to have been isolated from Parenchinus angulosus, appeared by restriction enzyme mapping and partial sequencing to be identical to …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
… reagents has allowed systematic analysis of gene function. The experimental accessibility of budding yeast makes it a test-bed for technology development and application of new functional genomic tools and resources that pave the way for comparable efforts in higher eukaryotes. In this …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… suGF1 may be involved in the regulation of early histone gene expression by interaction with a nudeosome which has been shown to be positioned in vitro over the H1-H4 intergenic region of the early histone gene battery of Psammechinus miliaris (189, H.-G. Patterton & J.P. Hapgood, unpublished). In …
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Investigating the Biological Role and Binding Modes of Histone-Like Proteins of Halophilic Archaea
… In Archaea, the majority of species contain a histone fold domain-containing protein, and these have been shown to compact DNA through the formation of nucleosomes and extended structures called hypernucleosomes. However, the role of the histone-like proteins of halophilic archaea is unclear. …
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Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor
The upstream regulatory regions of numerous genes contain contiguous deoxyguanosine residues (G·C-rich sequences) which have been implicated in the regulation of gene expression, since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the …
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The effect of negative supercoiling on the formation and positioning of nucleosome cores in vitro
… (pHP2) containing a section of the early H1-H4 histone gene spacer of Psammechinus miliaris, previously shown to position the histone octamer (Retief et al., 1987, Biochemistry, 26, 4449-4453). It is shown for the first time, by determinations of the linking difference of reconstituted …
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Interphase chromosome movement during the midblastula transition in Drosophila melanogaster
… that global chromatin changes occur, such that gene expression and replication proceed. I undertook to directly observe the structural changes of interphase chromatin, at a time in Drosophila embryogenesis when many nuclear processes were just beginning to be established. I reasoned that cycle …
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Organization principles of the early Drosophila embryo
… of the fruit fly Drosophila melanogaster: the Histone Locus Body size control and cortical migration of nuclei. The Histone Locus Body (HLB) is an evolutionarily conserved nuclear body that regulates the transcription and processing of histone mRNAs. We show that Drosophila HLBs form through …
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Defining CP-CML patient subsets associated with poor imatinib uptake and response.
… achieve excellent clinical (haematological, cytogenetic and molecular) responses on imatinib, it is clear that a subset of patients will respond poorly, or fail imatinib therapy. Currently, up to 35% of patients treated with imatinib fit into this subset, displaying either primary or acquired …
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Die Regulation der humanen H3-Histongene
… replikationsabhängig-exprimierten H3-Histongene aufzuklären und Beziehungen zur in vivo-Expression der Gene zu untersuchen. Im ersten Teil der vorliegenden Arbeit wurde die in vivo-Expression der 11 H3-Histongene in drei humanen fötalen Geweben (Blase, Leber und Lunge) und acht humanen …