Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"histone chaperone"”.
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Regulation of the histone H3K36 methyltransferase Set2 by the histone chaperone Spt6
Histone H3 lysine 36 (H3K36) trimethylation is a conserved post-translational modification critical for maintaining eukaryotic transcriptional fidelity and genomic stability. This modification is catalyzed in yeast by Set2, an ortholog of the mammalian H3K36 methyltransferase SETD2. Several studies …
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Roles of the Histone Chaperone FACT in Drosophila Gene Regulation and Chromatin Architecture
The highly conserved histone chaperone FACT (Facilitates Chromatin Transcription) is thought to contribute to the disassembly and reassembly of nucleosomes in the wake of RNA polymerase II passage through chromatin. In yeast, mutation of FACT subunits leads to decreased nucleosome occupancy at the …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… chromatin. Key regulators of these processes are histone binding proteins known as histone chaperones. The histone chaperone complex Chromatin Assembly Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a DNA synthesis dependent manner. During replication independent assembly …
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THE INTERPLAY OF HISTONE CHAPERONE NAP1 WITH SWI/SNF FAMILY OF CHROMATIN REMODELLERS IN S.cerevisiae
Histone disassembly is one of the mechanisms by which the ATP dependent chromatin remodelers remodel the nucleosomes. However nucleosome remodeling is also regulated by other factors like covalent modifications marks (methylation, acetylation etc. of both histones and chromatin remodelers). and …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
… nucleus through the interaction with four core histone proteins, H2A, H2B, H3 and H4. These proteins organize the genetic material, however they also make it difficult for the cells to access the information stored within the DNA sequence for processes such as transcription and replication. One …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
<p>Histones are architectural proteins that wrap approximately two turns of DNA around their octameric core structure, constituting the fundamental packaging unit of eukaryotic chromatin — the nucleosome. Beyond their structural role, they regulate virtually all processes that act on or depend on …
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Characterisation of the (H3-H4)2-tetramer and its interaction with histone chaperones
Histones are a major protein component of chromatin, yet mechanisms which control synthesis, post translational modification, deposition and removal of histones are not fully understood. Although atomic resolution structures have been solved for the nucleosome core particle, there is limited …
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Insights into the molecular mechanisms of function and regulation of histone crotonylation
Histone posttranslational modifications (HPTMs) are major regulators of chromatin dynamics and gene expression. Knowledge about the molecular machineries involved in the regulation and interpretation of a HPTM is crucial for understanding their functional significance. Histone crotonylation is a …
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Role of Nuclear Hat1p Complex and Acetylation of Newly Synthesized Histone H4 in Chromatin Assembly
… through the acetylation of newly synthesized histone H4 on lysine 5 and 12, there has been lack of straightforward evidence linking Hat1p to this process. We utilized the yeast Gal-HO system to study the function of nuclear Hat1p-containing type B histone acetyltransferase complex (NuB4) in …
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Expression, purification and characterisation of recombinant chromatin assembly factor 1
… (CAF-1) is the only known replication dependant histone chaperone, responsible for the deposition of the histone H3/H4 tetramer onto DNA. Found in all eukaryotes, CAF-1 consists of three subunits, p150, p60 and p48. Since its identification work on CAF-1 has mainly focused on in vivo studies due …
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Regulatory network built by Cytochrome c with Histone Chaperones in response to DNA damage
… such as Protein Phosphatase 2A (PP2A). Moreover, histone chaperones assist the DNA repair mechanisms altering chromatin dynamics through their nucleosome assembly activity. Recent studies have reported that the mitochondrial respiratory protein cytochrome c is promptly translocated into the cell …
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Identification of MMS22 as a regulator of DNA repair
… MMS22L interacts with NF?BIL2/TONSL, the histone chaperone ASF1 and subunits of the MCM replicative helicase. MMS22L colocalizes with TONSL at perturbed replication forks and at sites of DNA damage. MMS22L and TONSL are important for the repair of collapsed replication forks as depletion …
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Functional Analysis of Daxx In Tumorigenesis of Pancreatic Neuroendocrine Tumors and Embryonic Development
<p>Death domain-associated protein 6 (Daxx) is a histone chaperone specific to Histone 3.3 (H3.3). DAXX interacts with ATRX forming a chromatin remodeling complex, which deposits H3.3 into telomeric and pericentric region of the genome. The importance of Daxx was manifested in embryonic …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… chromatin assembly factors (Acf1 and ISWI), core histones (H2A, H2B, H3, and H4), and a histone chaperone (NAP-1) in order to define the mechanism used by high-risk and low-risk HPV E6 to inhibit p53-dependent transcription. We demonstrate that E6 does not prevent p53 or p300 recruitment to the …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
… to demonstrate that Rlf2, a component of the histone chaperone CAF-1, is able to bind SUMO in S. cerevisiae. This raises the possibility that CAF-1 plays a role in SUMO-mediated transcriptional repression. Secondly, I used ATAC-Seq to demonstrate that SUMO system inhibition may increase …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
… this approach to probe cell cycle-regulation of histone gene transcription by introducing an HTA1 promoter-GFP reporter gene construct into an ordered array of ~4500 yeast deletion mutants. I scored defects in reporter gene expression for each mutant, generating a quantitative analysis of histone …
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Genome-Wide Localization And Novel Deposition Pathways Of Histone Variant H3.3 In Embryonic Stem And Neuronal Precursor Cells
… DNA, RNA, and closely associated proteins. Histone proteins form the core of the nucleosome, the fundamental repeating unit of chromatin. Variant histone proteins play critical roles in the epigenetic regulation of gene expression and in the development of multicellular organisms. In this …
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Hepatitis C Virus NS5A Targets the Nucleosome Assembly Protein NAP1L1 to Control the Interferon Response
… characterized in my laboratory. NAP1L1 is a histone chaperone protein with various functions related to nuclear chromatin remodelling that impact on the regulation of cell cycle, on cell differentiation and on transcription. I have confirmed the interaction of NS5A with NAP1L1 in the …
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Characterizing and targeting the genomic consequences of ATRX deficiency in glioma
<p>Mutational inactivation of histone chaperone ATRX (a-thalassaemia/mental retardation X-linked) represents a defining molecular feature in several cancers, including malignant glioma. As standard of care only leads to transient responses and poor outcomes, there is an unmet clinical need for …
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