Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"histone H4"”.
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A histone H4 code regulates 53BP1-mediated responses to DNA damage
Individual histone post-translational modifications have been implicated in regulating many cellular events. Modifications can occur at high densities in the N-terminal tail domains of core histones, enabling the possibility that combinations of modifications regulate chromatin processes …
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Role of Nuclear Hat1p Complex and Acetylation of Newly Synthesized Histone H4 in Chromatin Assembly
… through the acetylation of newly synthesized histone H4 on lysine 5 and 12, there has been lack of straightforward evidence linking Hat1p to this process. We utilized the yeast Gal-HO system to study the function of nuclear Hat1p-containing type B histone acetyltransferase complex (NuB4) in …
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Improved Dynamic Range, Quantitation, and Characterization of Histone H4 Post-Translational Modifications: A Top Down Mass Spectrometric Approach
"Intimately associated with DNA, histone proteins serve as both a structural scaffold for DNA packaging into the nucleus and an epigenetic means for the regulation of gene expression. One such histone-based mechanism for transcriptional regulation is post-translational modification (PTM) of …
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Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
<p>The histone lysine methyltransferases catalyze the transfer of methyl groups from S-adenosyl-L-methionine to specific ε-N-lysyl residues in the N-terminal regions of histones H3 and H4. These enzymes are located exclusively within the nucleus and are firmly bound to chromatin. The …
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Isolation and characterisation of histone transacetylases
… one of the post-synthetic modifications of histones, weakens histone-DNA interactions and may play a regulatory role in gene control of eukaryotes. The literature available on histone acetylation as well as other post-synthetic modifications of histone has been reviewed. Histone acetylation …
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Antitumor activity of antimalarials in human breast cancer cells
… 18 cytoskeleton was reorganized. Hyperacetylated histone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h. Quinidine treated MCF-7 cells showed elevated p21/WAF1, hypophosphorylation and suppression of retinoblastoma protein, and down-regulation of …
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Plant Homeodomain Finger Protein 20 (Phf20) and Its Homolog Phf20 Like 1 (Phf20L1) Define Two Distinct Non-Specific Lethal (Nsl) Complexes
… complex, which acetylates lysine residues on histone H4 and regulates gene expression. The current model assumes that PHF20 and PHF20L1 are present together in the NSL complex, although it has never been tested. Performing extensive biochemical analysis, we observed that PHF20 and PHF20L1 were …
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Roles of H2A.z in Fission Yeast Chromatin
Covalent histone modifications such as methylation, acetylation as well as differential incorporation of histone variants are shown to coincide with different chromatin compartments and mark active or repressed genes. Msc1 is one of the seven JmjC Domain Proteins (JDPs) in Fission Yeast. JDPs are …
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Characterization of the histone deacetylation activity of the yeast silencing gene SIR2
… for maintaining a hypoacetylated state of the histones at these locations. To silence genes, the Sir2 protein can deacetylate histones in the presence of NAD+. This is shown by the ability of Sir2p to remove the acetyl groups from peptides corresponding to the amino-terminal tail of histone H3 …
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Transcriptional Regulatory Mechanisms of Ribosomal Protein Genes
… NuA4 (Nucleasome acetyltransferase of histone H4) complex to activator Rap1. NuA4 enhances PIC (Pre-initiation complex) formation at the core promoter, but it is not clearly understood how does it do so. Researches have identified two different forms of TBP: TAF (TBP associated …
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Targeting Epigenetic Regulators For The Treatment of Diffuse Large B-Cell Lymphoma
<p>Small-molecule inhibitors of the histone methyltransferase EZH2 hold great promise for the treatment of Germinal Center B-Cell-like Diffuse Large B-Cell Lymphoma (GCB-DLBCL). Compared to a 60% Objective Response Rate (ORR) in Phase I clinical trials, Phase II trial results for the EZH2 inhibitor …
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Identification and characterisation of phytophthora capsici CRN effector proteins and their targets in planta
… 3’phostphate dehydrogenase, Fibrillarin 2 and histone H4, indicate the potential involvement of CRNs in altering transcription machinery of the host plant; all targets can be linked to transcriptional complexes in plants.<br/>We conclude that CRNs are likely to play an important role in the …
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Microbiota regulate short chain fatty acids and influence histone acylations in intestinal epithelial cells.
… cells. SCFAs are chemically related to histone acylations, a growing number of post-translational modifications that include histone acetylation, butyrylation and crotonylation. Histone post-translational modifications are thought to be involved in the regulation of gene expression as …
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The role of Xist RNA in the maintenance of X chromosome inactivation
… silent, late replicating, and hypoacetylated on histone H4, confirming that X-inactivation can be maintained in the absence of Xist RNA. However, the Xist mutant inactive X was no longer enriched in histone macroH2A 1. Furthermore, the reactivation rate of GFP and Hprt increased, indicating Xist …
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Protein O-Kinases in the Archaeon Sulfolobus solfataricus
… and maleylated lysozyme (RCM lysozyme), histone H4 proved, and a peptide modeled after myosin light chains (KKRAARATSNVFA) were phosphorylated to appreciable levels <i>in vitro</i>. All of the aforementioned substrates were phosphorylated on threonine, while histone H4 was phosphorylated …
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Placental epigenetic modifications and development in valproic acid-induced teratogenesis
… 24 h post-dosing, to characterize the profile of histone modifications and measure DNA methylation levels. Moreover, we exposed pregnant mice to 600 mg/kg of VPA or saline on GD9 or GD13 and collected placental and fetal tissues on GD18, to assess placental growth and development as well as fetal …
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ROLES OF ACCESSORY DOMAINS IN THE CATALYTIC SUBUNIT OF ISW2
… of regulation that are independent of the H4 tail. We deleted SANT domain (ΔSANT) and also created mutations in three basic amino acid residues (mSANT) on the surface of SANT domain predicted to be interacting with nucleosomal DNA. The absence of SANT domain affected ISW2 activity neither …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… superhelical locations that engage and remodel histone H4 tails. These interactions reposition histone tails and displace linker histone H1, resulting in chromatin destabilization and increased DNA accessibility. Altogether, this thesis provides structural and mechanistic insights into …
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Epigenetic Changes in Alzheimer’s and Huntington’sS Disease : A semi-quantitative analysis of histone changes
Histone acetylation is an epigenetic mechanism that plays a critical role in the regulation of gene transcription, DNA damage repair and DNA replication. Histone acetylation homeostasis is maintained by a balance in the activity of histone deacetylases (HDAC) and acetyltransferases. Disruption in …
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