Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 141 for “"histone H3"”.
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Regulated Histone H3 Proteolysis During Mouse Embryonic Stem Cell Differentiation
<p>The association of genomic DNA with histone proteins in the three-dimensional structure known as chromatin is the central framework for "epigenetics," which is defined as inherited phenotypes governed by differences that cannot be explained by changes in DNA sequence. In recent years, studies …
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Investigating a novel function of histone H3 in mitotic checkpoint control in Saccharomyces cerevisiae
Thesis (Ph. D.)--Michigan State University. Dept. of Biochemistry and Molecular Biology, 2010
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… chromatin. Key regulators of these processes are histone binding proteins known as histone chaperones. The histone chaperone complex Chromatin Assembly Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a DNA synthesis dependent manner. During replication independent assembly …
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Regulation of the Heterochromatin Protein 1 by Phosphorylation of Histone H3 and the HP1 Hinge Domain
<p>Chromatin, a polymer formed from DNA, histones, and associated proteins, is the physiological form of genetic information in all eukaryotic cells. Posttranslational modification of histones, such as acetylation, methylation, and phosphorylation, regulates various DNA-dependent processes, ranging …
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Mechanistic Insights into the Stimulation of Dot1L-Mediated Methylation of Histone H3 by Semisynthetically Ubiquitylated Histone H2b
<p>Post-translational modification of histones plays an integral role in regulation of chromatin-templated processes through modulation of chromatin structure and function. One such modification, ubiquitylation of histone H2B on lysine 120 (uH2B), has been correlated with enhanced methylation of …
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Investigating histone H3 Lys4 and Lys9 methylation in conditionally immortalized olfactory placode by matrix-assisted laser desorption
… have shown that distinctive site-specific histone H3 methylation patterns at lysine 9 and lysine 4 determine the formation of heterochromatin oreuchromatin, respectively. The biological significance of lysine 4 and lysine 9 methylation of histone N-terrriinal tails was investigated in this …
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
<p>Histone H3K4 methylation is conserved across species and is associated with active transcription. By using <em>Saccharomyces cerevisiae</em>, we found histone H3K4 methylation has a previously unknown role in regulating mitosis through the Spindle Assembly Checkpoint. The Spindle Assembly …
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Methylation Change of Histone H3 and CpG Sites at the TIM-3 Gene Promoter in Response to Stimulation
… DNA 메틸화 변화는 일어나지 않았다. 다음으로 TIM-3 promoter에서의 히스톤 H3 메틸화는 PBA와 A23187 자극에 의한 T 세포를 활성화 시켰을 때 proximal TIM-3 promoter (-954 ~ -34) 부위에서 H3K4me2의 수준이 증가되었고 distal TIM-3 promoter (-1549 ~ -1048) 부위에서 H3K9me3와 H3K27me3의 수준이 증가되었다. 본 연구의 결과는 TIM-3 전사 조절을 이해하는데 중요한 기초로 제공될 것이다.
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Characterization of interplay among DNA repair, histone H3 lysine 9 dimethylation and small interfering RNA pathway in Caenorhabditis elegans
… maintain genome integrity in different species. Histone H3 lysine 9 dimethylation (H3K9me2) has been shown to play an important role in mediating the formation of heterochromatin. In our lab, we use Caenorhabditis elegans as a model system to study the biological significance of the enrichment of …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… Many nuclear proteins were found, in particular histone H3 variants. Histones H3.1, H3.2 and H3.3 were demonstrated to be modified with fatty acid chemical reporters on the conserved cysteine 110, a novel site of S-acylation on histone H3.2. This newly discovered modification of histone H3 …
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Discovery and Characterization of Methylation of Arginine 42 on Histone H3: A Novel Histone Modification with Positive Transcriptional Effects
… turns of DNA wrapped around an octamer of core histone proteins composed of four histone species: one histone H3/H4 tetramer and two histone H2A/H2B dimers. Histones are basic globular proteins rich in lysine and arginine residues, with unstructured N-terminal "tail" regions protruding outside …
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Investigation of parameters of chromatin higher order structure and the making of multiply-acetylated histone H3 via nonsense suppression
… This is an especially ubiquitous PTM in the histones of chromatin, and is important for helping to regulate both structural and mechanistic aspects of chromatin. A number of strategies exist for generating acetylated nucleosomes for the in-vitro study of chromatin though they all have various …
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Investigating the role of histone H3 lysine 9 dimethylation in regulating disease-associated vascular smooth muscle cell gene expression
… remain unclear. This thesis explores the role of histone 3 lysine 9 dimethylation (H3K9me2), a repressive epigenetic mark, in regulating the expression of disease-associated VSMC genes. Intriguingly, murine models of VSMC phenotypic switching revealed reduced levels of H3K9me2 upon loss of the …
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Toward an epigenetic classification of human testicular germ cell tumors (TGCTs): preliminary assessment of histone H3-K27 trimethylation according to TGCT subtype, aggressiveness, and response to therapy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01
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Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
<p>The histone lysine methyltransferases catalyze the transfer of methyl groups from S-adenosyl-L-methionine to specific ε-N-lysyl residues in the N-terminal regions of histones H3 and H4. These enzymes are located exclusively within the nucleus and are firmly bound to chromatin. The …
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Chromatin and transcriptional regulators act in a cascade to establish a bilateral asymmetry of the C. elegans nervous system
… that a mutation in a replication-dependent histone H3 gene his-9 transforms the MI neuron into an e3D-like cell. This mutant allele of his-9 causes an altered-function activity that is predicted to impair the interaction of the mutant HIS-9 protein with another histone H3 molecule and …
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From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins
… large peptides to characterize PTM profiles on histone H3. Interestingly, we were able to conclude that methylation and acetylation levels on histone H3 are highly effected by perturbations of DNA methylation. In the past years we have expanded the applications of top down and middle down MS to …
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Polo-Like Kinase 1 and Smooth Muscle Cells in Coronary Artery Bypass Conduits and Coronary Arteries Following Angioplasty
… kinase (PLK)-1 in the PLK1/pPLK1-pCDK1-p-Histone H3 pathway in mediating mitotic progression of smooth muscle cells (SMCs) from human IMA and SV in the development of IH leading to veingraft disease. We also show in vivo data in support of increased immunopositivity towards PLK1 and pPLK1 …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… monitoring post-translational modifications of histone H3 in living cells was developed using genetically encoded fluorescent reporters. These reporters were constructed for sensing histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These reporters are …
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