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Showing 1 to 20 of 30 for “"histone H1"”.
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Towards characterisation of histone H1 gene transcription factors / by Blair Hopwood.
Bibliography : leaves 165-178.
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Physical and biochemical studies of histone H1 and its globular domain
The interaction of the histone H5 globular domain with DNA and chromatin was studied using several physical and chemical techniques, including electron microscopy, dynamic light scattering, equilibrium density and velocity ultracentrifugation, and chemical crosslinking.
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A role for Hho1p, a histone H1 homologue, in Saccharomyces cerevisiae
It is well established that histone H1 is involved in facilitating the higher-order structures of chromatin. It has not become possible to test the biochemical function of H1 in Saccharomyces cerevisiae, since the identification of a H1 homologue (Landsman, 1996) with properties of a bona fide …
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Regulation of Chromatin Multi-Scale Organization and Function by Linker Histone H1
… factor that appears to play a key role is linker histone H1, which is the most prevalent chromatin-binding protein in the cell. It has long been thought to be responsible for compacting chromatin and repressing transcription, though many historical studies of H1 were performed in vitro, and the …
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Regulation and function of interphase histone H1 phosphorylation in pluripotent cell differentiation
Histone H1 phosphorylation is thought to be involved in multiple cellular processes including chromatin condensation and transcriptional regulation. Recent studies revealed changes in the expression and genomic distributions of H1 variants during cell differentiation which appear to contribute to …
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Site-specific phosphorylation of histone H1 associated with cell cycle progression and transcription
Histone H1 phosphorylation is thought to affect chromatin condensation and function, but few details are known about the impact of H1 variant-specific phosphorylation in higher eukaryotes. Using novel proteomic approaches, we directly demonstrate that specific sites in the two dominant H1 variants …
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Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III
The linker histone HI binds to the nucleosome and is essential for the organization of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very …
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The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme
… This project is a study of the very lysine-rich histone fraction or H1-histone fraction of Parechinus angulosus embryo. The first part deals with the characterization and separation of the H1-variants present in the late gastrula embryo. The second part describes the determination of the partial …
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The molecular and biochemical characterization of cell cycle regulators in Pisum sativum
… in other systems, these proteins copurify with a histone H1 kinase activity by p13$\sp{suc1}$-Sepharose affinity chromatography. p13$\sp{suc1}$-associated histone H1 kinase activity is elevated and a set of endogenous proteins is phosphorylated specifically at the S and M phases. Cell cycle …
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CRAMP1 drives linker histone expression to enable Polycomb repression
The essential role of the core histones (H2A, H2B, H3 and H4) in DNA packaging has been well-understood for decades, but the function of the linker histone (H1) remains enigmatic. H1 compacts nucleosome arrays in vitro, but the multiple H1 subtypes and variants encoded in mammalian genomes (11 in …
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Novel Roles of the Aryl Hydrocarbon Receptor in Normal Physiology and Epigenetic Reprogramming
… results in homocitrullination of linker histone H1 lysine 34 (H1K34hcit). Homocitrullination, a novel epigenetic mark, was observed only in response to exogenous AhR agonists and is absent upon treatment with endogenous AhR ligands such as cinnabarinic acid. Results presented here …
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Leishmania CRK3:CYC6 cyclin-dependent kinase as a drug target
… CYC6 to produce a protein kinase complex with histone H1 kinase activity. CRK3his and CYC6his were co-expressed and co-purified from Escherichia coli via metal affinity and gel filtration chromatography to obtain a 1:1 ratio of CRK3:CYC6 proteins, which formed a stable protein kinase complex. …
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Analysis of cyclin dependent kinases in Leishmania
… CRK3 in an in vitro kinase assay using histone H1 as substrate. The CRK3/CYCA active complex was then used to analyse the effect of the phosphorylation at the CRK3 activation threonine using a kinase activating kinase (yeast CAK or Civ-1). Phosphorylated CRK3 activity was compared to …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… thought that PRC2 propagates repressive histone marks by modifying neighboring nucleosomes in a strictly linear progression. However, the behavior of PRC2 on native-like chromatin substrates remains incompletely characterized, making the precise mechanism of PRC2-mediated heterochromatin …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… poorly understood. Here, we find that HP1BP3, a histone H1-like chromatin protein, specifically associates with the Microprocessor and promotes global miRNA biogenesis in HeLa cells. Accordingly, chromatin immunoprecipitation (ChIP) studies reveal genome-wide co-localization of HP1BP3 & Drosha …
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Androgen Regulation of Gene Expression Associated with Cell Growth and Neural Plasticity: Studies in Songbird Brain and the S115 Cell Line
… ionophore A23I87 to induce c-myc and histone H1 expression, where neither alone will do so. In addition, androgen pretreatment represses the TPA-induced increase of these same genes.</p>
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WHO: A Novel Wuschel-Like Homeodomain Transcription Factor in Arabidopsis
… including pivotal developmental genes such as HISTONE H1, CUP-SHAPED COTYLEDON1 (CUC1), CLAVATA3 (CLV3 ) and SHOOTMERISTEMLESS (STM). Taken together, these findings indicate that the misexpression of WHO caused by promoter element disruption affect fundamental developmental programs in …
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Investigations into the role of histone H2A ubiquitination in chromatin
… vitro system was used to determine the effect of histone H2A ubiquitination on linker histone binding to mononucleosomes. Hybrid octamers containing either H2A or ubiquitinated H2A (uH2A) were reconstituted onto random sequence 167 bp DNA. The affinity of the resultant nucleosome cores for linker …
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STRUCTURAL INSIGHTS INTO RNA POLYMERASE III PRE-INITIATION COMPLEX AND THE PIONEER ROLE OF OCT1
… superhelical locations that engage and remodel histone H4 tails. These interactions reposition histone tails and displace linker histone H1, resulting in chromatin destabilization and increased DNA accessibility. Altogether, this thesis provides structural and mechanistic insights into …
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The genetic and molecular analysis of the mfs(2)31 locus in Drosophila melanogaster : a novel suppressor of position-effect variegation
… and E(var)s), identify loci which encode non-histone chromosomal proteins. Although the majority of mutations which modify PEV exhibit a dominant phenotype, rare recessives u(var) mutations have been reported. This study describes a locus which is identified by one such recessive mutation, …
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