Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"high-content screening"”.
-
OrganixInsights: High throughput imaging and high content screening of organoids
… and extracellular matrix interactions. However, high-resolution imaging of organoids generates large volumetric datasets that require advanced computational methods for analysis. This dissertation presents a comprehensive computational framework for high-content screening and quantitative …
-
Multivariate methods for the statistical analysis of hyperdimensional high-content screening data
… increasingly complex datasets. In recent years, high-content screening (HCS) using RNA interference (RNAi) or other perturbation techniques in combination with automated microscopy has emerged as a promising investigative tool to explore intricate biological processes. Image-based HC screens …
-
Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… yeast cell morphology and yeast cell cycle using high content screening with a high-capacity automated imaging system. The images obtained using this method can also provide information about fluorescently labelled proteins, unlike flow cytometry, which can only measure overall fluorescent …
-
An apparatus for high throughput muscle cell experimentation
… heart. The myocyte experimental model provides a higher level of physiological relevance than molecular or myofibril studies while avoiding problems inherent to multicellular preparations including heterogeneity of cell types and diffusion limited extracellular spaces. Millions of primary myocytes …
-
Automatic Computational Techniques for Image Processing Problems
… the method in drug development industry for high content screening. Starting from the traditional statistics-based method, we then investigate a more mathematical model, the total-variation (TV) method for image denoising and deblurring problems. In traditional TV-based models, it is not easy …
-
Novel Neuroblastoma Differentiating Agents
… (NB) is the 3rd most lethal pediatric cancer. In high-risk patients, the 5-year survival rate is 40-50% after treatment. The differentiation therapy with retinoic acid (RA) is at the forefront of approaches to treat neuroblastoma; however, more than half of patients experience recurrence …
-
Bayesian level sets and texture models for image segmentation and classification with application to non-invasive stem cell monitoring
… acceptable performance, even in cases with high signal to noise and interference ratio that do not necessitate restoration. This weakness impedes application of image analysis to many important fields, such as automated, mass scale cultivation and non-invasive, non-destructive high …
-
Multi-channel solutions for optical labelfree detection schemes based on the interferometric and grating coupler principle
… two important fields of application, namely the high throughput screening and the so called high content screening with two different optical labelfree sensor approaches. Cheap disposable sensor units are of importance for HTS. Here embossed gratings which may act as transducers or coupling …
-
Methods and technologies for high-throughput and high-content small animal screening
High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to …
-
Development and Application of a Novel Assay for Rapid Identification of Cellular Prion Protein Modulators
… application of an innovative BiFC-based in vitro screening assay, Light-Identification of Protein Suppressors (LIPS), designed for real-time monitoring of PrPC expression and modulation. The LIPS system is based on the Bimolecular Fluorescence Complementation (BiFC) technique, usually used for …
-
An integrated approach to ciprofloxacin susceptibility analysis and high-throughput bacterial phenotyping in Salmonella
… inhibitory concentration (MIC). I have developed high-content imaging methodologies to screen Salmonella grown in the presence of ciprofloxacin. These morphological data suggest that there may be heterogeneous subpopulations with differential responses to ciprofloxacin, which was supported by …
-
A NOVEL PIPELINE FOR DRUG DISCOVERY IN NEUROPSYCHIATRIC DISORDERS USING HIGH-CONTENT SINGLE-CELL SCREENING OF SIGNALLING NETWORK RESPONSES EX VIVO
… current work entails the development of a novel high content platform for the measurement of kinetic ligand responses across cell signalling networks at the single-cell level in distinct PBMC subtypes ex vivo. Using automated sample preparation, fluorescent cellular barcoding and flow cytometry …
-
Cross-Modality Profiling of High-Content Microscopy Images with Deep Learning
… to integrate metadata labels freely obtained in high-content screening into deep learning architectures. The use of automated microscopy in high-content phenotypic screening of cells treated with compounds or genetic perturbations produces a large amount of high-dimensional data. One example …
-
Antibacterial activity of the crude extract and fractions of spirostachys africana against multi-drug resistant bacteria
Background: The high on-going incidences of infectious diseases, specifically those caused by multi-drug resistant bacteria in the last decade has made it a necessity to investigate a variety of antimicrobial drug sources, such as plants. Medicinal plants have played a significant role in drug …
-
TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
… PIK3C3 complexes. In parallel studies, we used high content (HC) screening approaches to identify small molecules able to ameliorate the MLIV phenotype. In one of them, we tested whether previously identified drugs (150 FDA compounds) inducing TFEB translocation and cellular clearance might be …
-
Functional miRNA-based Phenotypic Screening as a tool to delineate HIV-host interactions and facilitate Novel Drug Discovery
… pathogen that encodes only 15 proteins HIV-1 is highly dependent upon its host's cellular machinery in order to complete its life cycle. Host-directed therapy thus represents a potentially lucrative strategy for the development of novel anti-HIV therapies. microRNAs (miRNAs) are short noncoding …
-
Pancreatic Cancer - Early Detection, Prognostic Factors, and Treatment
… and healthy controls (n=9) were analyzed by high definition mass spectrometry (HDMSE). In paper II, an artificial neural network (ANN) model was constructed on 84 pancreatic cancer patients undergoing surgical resection. In paper III, we investigated the effects of transition from a low- to a …