Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"high-content microscopy"”.
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Cross-Modality Profiling of High-Content Microscopy Images with Deep Learning
… to integrate metadata labels freely obtained in high-content screening into deep learning architectures. The use of automated microscopy in high-content phenotypic screening of cells treated with compounds or genetic perturbations produces a large amount of high-dimensional data. One example …
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Using Chemical Probes to Examine Cellular Activities
… morphological markers of drug resistance via high-content microscopy. In the first part of my thesis, I use chemical probes to examine spastin's role in cell division. Spastin is a AAA (ATPases associated with diverse cellular activities) protein that is recruited in anaphase by the endosomal …
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Identification and Characterization of MicroRNAs Modulating Cardiac Hypertrophy
… and preserve cardiac function, we performed a high-content microscopy, high-throughput functional screening for human microRNAs able to reduce neonatal cardiomyocyte (CM) cell size using a whole-genome microRNA library. The most effective anti-hypertrophic microRNA was hsa-miR-665. In a model …
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Functional Identification of MicroRNAs Counteracting Cellular Senescence
… of 879 human miRNAs mimics was used for a high throughput screen using high content microscopy to search for miRNAs able to restore a high replicative phenotype in WI-38 human diploid fibroblasts exhibiting replicative senescence. Screening endpoints were the analysis of the incorporation …
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Genome-wide microscopy screening identifies links across processes including a conserved connection between DNA damage control and the microtubule cytoskeleton
… in the lab created a method for large-scale, high-content microscopy screening of a cell library consisting of over 3000 single mutant strains of the fission yeast, Schizosaccharomyces pombe. Each strain has one nonessential gene knocked-out, allowing investigation of the resulting phenotypes. …
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Investigating Mechanisms In Nociceptors Driving Ongoing Activity and Ongoing Pain
… patch clamp electrophysiology, pharmacology, and high content microscopy was used to define the effects of low concentrations of the injury-related proinflammatory mediator serotonin and the major pathway by which it exerts its effects. This study shows that serotonin enhances DSFs, hyperpolarizes …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
… validation method which combined arrayed high-content microscopy and semi-automated image analysis to confirm a novel set of host factors which facilitate *Shigella* infection. I then established in greater detail the roles of three of the major identified hits, using a further panel of …
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OrganixInsights: High throughput imaging and high content screening of organoids
… and extracellular matrix interactions. However, high-resolution imaging of organoids generates large volumetric datasets that require advanced computational methods for analysis. This dissertation presents a comprehensive computational framework for high-content screening and quantitative …