Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"high-content imaging"”.
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Interplay of Brain Derived Neurotrophic Factor and Protein Kinase A in Modulating Mitochondrial Structure and Function in Brain Homeostasis and Disease
… elongation and increase the trafficking and content of mitochondria in dendrite trees. Secondly, exposure of neurons to BDNF enhanced neuronal metabolism (oxidative phosphorylation and glycolysis) and increased the transmembrane potential of mitochondria; processes that required activation of …
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An integrated approach to ciprofloxacin susceptibility analysis and high-throughput bacterial phenotyping in Salmonella
… inhibitory concentration (MIC). I have developed high-content imaging methodologies to screen Salmonella grown in the presence of ciprofloxacin. These morphological data suggest that there may be heterogeneous subpopulations with differential responses to ciprofloxacin, which was supported by …
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INHIBITING THE NUCLEAR RECEPTOR ESRRA RESCUES CORE PATHOLOGIES IN CELLULAR AND ANIMAL MODELS OF BATTEN DISEASE
… genes involved in Gb3 buildup, we performed a high-content imaging screen using a library of approximately 11,000 siRNAs (small interfering RNAs, which silence specific genes) in a human ARPE-19 CLN3 knockout (CLN3KO) cell model. The survey identified 84 candidate genes whose depletion reduced …
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In vitro evaluation of Ultraviolet Radiation (UVR) exposure of the Retinal Pigmented Epithelium (RPE) and its relevance to Age-Related Macular Degeneration (AMD)
… toxicity. Finally, a cellpaint-like high content imaging assay was developed in order to, at once, quantify intracellular oxidative stress, nuclear morphology, mitochondrial stress, epithelial integrity and cell viability as part of a cellprofiling approach to quantifying UVR …
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High throughput measurement and perturbation of tissues and tissue-derived cellular models
… screening” approach in the U2OS cell line with a high-content imaging (Cell Painting) readout and a bioactive small molecule perturbation library. We then applied this method to identify novel microenvironmental factors that modify RNA state in pancreatic ductal adenocarcinoma (PDAC) organoids. …
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Applying at scale genomic and phenotypic approaches to study pathogens linked to nosocomial transmission and disease
… viruses and global pandemics, all of which cause high levels of morbidity, mortality and burden on healthcare systems. In this thesis I have investigated three pathogens that are archetypes of these threats: the Gram-negative bacterium Klebsiella pneumoniae, the seasonal respiratory virus …
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Platforms for exploring host-pathogen interactions in hepatitis C virus infection
… with suboptimal specificity, sensitivity, and content. Here, we describe three technologies for overcoming these limitations in the study of host-virus interactions. We demonstrate their utility in exploring innate immune signaling, a clinically significant component of HCV pathogenesis. …
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Defining the mechanism of arsenic-induced degradation of PML
… process of arsenic induced degradation of PML, a high content siRNA screen was designed to monitor the fate of a YFP linked version of PML after siRNA mediated knockdown of components of the ubiquitin system and arsenic treatment. RNF4 depletion prior to arsenic treatment prevented PML degradation …
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Phenotypic screening and AI uncovers modulators of YAP and TAZ activity in glioblastoma
… of therapeutics for GBM is hampered by a highly complex disease that displays vast amounts of inter and intra-patient heterogeneity. This is enhanced by phenotypic plasticity of tumour cells which allows them to differentiate and regress into multiple glial-like cell types with neoplastic …
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Decoding CD4+ T cell activation by multi-modal phenotyping
… chapter, I outline the development of TGlow: a high-throughput high-content image-based method to profile morphology, metabolic, proteomic and functional features of T cell activation. In the third chapter, I implement TGlow to characterise CD4+ T cell activation with high temporal resolution in …